##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
Alw44I ApaLI AscI Asp700I AviII
AvrII BbeI BlnI BsaBI BsaHI
BsiEI BsiWI BspDI BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraIII
EagI EclXI Eco52I EcoO65I EcoRI
FseI FspI Hin1I HpaI KasI
KspI MamI MluI MvnI NarI
NotI NruI NspV PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 3144 TCCGGA T^CCGGA
AflII 4828 CTTAAG C^TTAAG
Aor51HI 4775 AGCGCT AGC^GCT
BamHI 4497 GGATCC G^GATCC
BbrPI 4504 CACGTG CAC^GTG
BcgI 3415 GCAAATCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1191 GTATCC GTATCC(6/5)
BfrI 4828 CTTAAG C^TTAAG
BsaAI 4504 CACGTG [CT]AC^GT[GA]
BseAI 3144 TCCGGA T^CCGGA
BsgI 391 CTGCAC GTGCAG(16/14)
BsmBI 2440 CGTCTC CGTCTC(1/5)
BspEI 3144 TCCGGA T^CCGGA
BspHI 4330 TCATGA T^CATGA
BspLU11I 3984 ACATGT A^CATGT
DraI 2000 TTTAAA TTT^AAA
Eco47III 4775 AGCGCT AGC^GCT
EcoRV 4743 GATATC GAT^ATC
FauI 3547 CCCGC CCCGC(4/6)
MroI 3144 TCCGGA T^CCGGA
NaeI 5080 GCCGGC GCC^GGC
NdeI 4658 CATATG CA^TATG
NgoMIV 5080 GCCGGC G^CCGGC
NheI 1498 GCTAGC G^CTAGC
PaeR7I 3423 CTCGAG C^TCGAG
PmaCI 4504 CACGTG CAC^GTG
PmlI 4504 CACGTG CAC^GTG
PshAI 1395 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4330 TCATGA T^CATGA
SmaI 385 CCCGGG CCC^GGG
SphI 928 GCATGC GCATG^C
SspI 2145 AATATT AAT^ATT
StuI 4866 AGGCCT AGG^CCT
XhoI 3423 CTCGAG C^TCGAG
XmaCI 385 CCCGGG C^CCGGG
XmaI 385 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1026 GGTACC G^GTACC
4161 GGTACC
AhdI 2951 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3536 GACTTCCAGTC
ApaI 857 GGGCCC GGGCC^C
4183 GGGCCC
Asp718I 1026 GGTACC G^GTACC
4161 GGTACC
AspEI 2951 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3536 GACTTCCAGTC
BbsI 2473 GAAGAC GAAGAC(2/6)
3173 GAAGAC
BclI 3597 TGATCA T^GATCA
4488 TGATCA
BglI 3189 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5101 GCCAGTGGGGC
BlpI 4024 GCTGAGC GC^T[ACGT]AGC
4137 GCTGAGC
Bpu1102I 4024 GCTGAGC GC^T[ACGT]AGC
4137 GCTGAGC
BpuAI 2473 GAAGAC GAAGAC(2/6)
3173 GAAGAC
BsaWI 2766 TCCGGT [AT]^CCGG[AT]
3144 TCCGGA
BssSI 4384 CACGAG CACGAG(-5/-1)
534 CTCGTG
BstXI 3444 CCAACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5008 CCAGGAAGTTGG
CelII 4024 GCTGAGC GC^T[ACGT]AGC
4137 GCTGAGC
DrdI 818 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2432 GACACAAGCGTC
Eam1105I 2951 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3536 GACTTCCAGTC
EcoT22I 2376 ATGCAT ATGCA^T
2745 ATGCAT
FbaI 3597 TGATCA T^GATCA
4488 TGATCA
HaeII 3609 AGCGCC [GA]GCGC^[CT]
4775 AGCGCT
HincII 1448 GTCGAC GT[CT]^[GA]AC
1764 GTCGAC
HindII 1448 GTCGAC GT[CT]^[GA]AC
1764 GTCGAC
HindIII 1359 AAGCTT A^AGCTT
2053 AAGCTT
KpnI 1026 GGTACC GGTAC^C
4161 GGTACC
MfeI 564 CAATTG C^AATTG
3552 CAATTG
MunI 564 CAATTG C^AATTG
3552 CAATTG
NsiI 2376 ATGCAT ATGCA^T
2745 ATGCAT
SalI 1448 GTCGAC G^TCGAC
1764 GTCGAC
SapI 3314 GAAGAGC GCTCTTC(1/4)
4907 GCTCTTC
SbfI 3238 CCTGCAGG CCTGCA^GG
3766 CCTGCAGG
ScaI 990 AGTACT AGT^ACT
3939 AGTACT
Sse8387I 3238 CCTGCAGG CCTGCA^GG
3766 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AspI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PshBI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoII