##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AviII BfrI BlpI
Bpu1102I BsaBI BsiWI BspDI BspHI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI DraIII Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HpaI KpnI MamI MfeI
MunI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
SalI SapI SbfI ScaI SexAI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1705 GACGTC GACGT^C
AocI 127 CCTGAGG CC^T[ACGT]AGG
Aor51HI 180 AGCGCT AGC^GCT
Asp700I 1053 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1393 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 416 GGCGCC GGCGC^C
BbrPI 3167 CACGTG CAC^GTG
BglII 5008 AGATCT A^GATCT
Bsp1407I 1226 TGTACA T^GTACA
BspLU11I 4478 ACATGT A^CATGT
BsrGI 1226 TGTACA T^GTACA
BssHII 171 GCGCGC G^CGCGC
BstAPI 150 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 127 CCTGAGG CC^T[ACGT]AGG
CpoI 11 CGGACCG CG^G[AT]CCG
DrdI 1636 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 180 AGCGCT AGC^GCT
Eco81I 127 CCTGAGG CC^T[ACGT]AGG
EcoNI 4176 CCTCAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 3950 AAGCTT A^AGCTT
KasI 416 GGCGCC G^GCGCC
MluI 1055 ACGCGT A^CGCGT
NaeI 1415 GCCGGC GCC^GGC
NarI 416 GGCGCC GG^CGCC
NdeI 4926 CATATG CA^TATG
NgoMIV 1415 GCCGGC G^CCGGC
PflFI 1393 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3167 CACGTG CAC^GTG
PmlI 3167 CACGTG CAC^GTG
PshAI 3850 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 11 CGGACCG CG^G[AT]CCG
SfoI 416 GGCGCC GGC^GCC
SgrAI 95 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1226 TGTACA T^GTACA
Tth111I 1393 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1455 TCTAGA T^CTAGA
XmnI 1053 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1055 ACGCGT A^C[GA][CT]GT
4478 ACATGT
AvrII 1503 CCTAGG C^CTAGG
4196 CCTAGG
BanI 416 GGCGCC G^G[CT][GA]CC
1594 GGTGCC
BbsI 864 GAAGAC GAAGAC(2/6)
1144 GAAGAC
BcgI 352 GCAGGACGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1254 GCAGAAATATCG
BciVI 1677 GGATAC GTATCC(6/5)
4629 GGATAC
BlnI 1503 CCTAGG C^CTAGG
4196 CCTAGG
BpuAI 864 GAAGAC GAAGAC(2/6)
1144 GAAGAC
BsaAI 1032 CACGTA [CT]AC^GT[GA]
3167 CACGTG
BsaI 1114 GAGACC GGTCTC(1/5)
3070 GAGACC
BsmBI 3868 CGTCTC CGTCTC(1/5)
1703 GAGACG
BspMI 1665 GCAGGT ACCTGC(4/8)
4073 GCAGGT
BsrBI 24 CCGCTC CCGCTC(-3/-3)
479 CCGCTC
BsrDI 658 GCAATG GCAATG(2/0)
1777 GCAATG
BstXI 2026 CCATCGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2602 CCATCGGTATGG
EagI 195 CGGCCG C^GGCCG
520 CGGCCG
EclXI 195 CGGCCG C^GGCCG
520 CGGCCG
Eco52I 195 CGGCCG C^GGCCG
520 CGGCCG
HincII 1441 GTTGAC GT[CT]^[GA]AC
4077 GTCAAC
HindII 1441 GTTGAC GT[CT]^[GA]AC
4077 GTCAAC
PpuMI 1875 AGGACCC [GA]G^G[AT]CC[CT]
5024 AGGTCCT
SacI 3184 GAGCTC GAGCT^C
4269 GAGCTC
SfiI 527 GGCCCCTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
930 GGCCAGCTCGGCC
XcmI 4562 CCAAGTAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4992 CCAACCCGAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanII BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspEI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI