##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AclI        AflII       AflIII      AgeI        
AhdI        AocI        AscI        AseI        AsnI        
AspEI       AvrII       BclI        BfrI        BglII       
BlnI        BsaBI       BsiWI       BsmI        BspLU11I    
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        MamI        MfeI        
MluI        MunI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SalI        SapI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SspI        
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AccIII       3886  TCCGGA               T^CCGGA
AcsI         1193  GAATTC               [GA]^AATT[CT]
Alw44I       1423  GTGCAC               G^TGCAC
ApaLI        1423  GTGCAC               G^TGCAC
ApoI         1193  GAATTC               [GA]^AATT[CT]
BseAI        3886  TCCGGA               T^CCGGA
BsiEI        1131  CGGTCG               CG[GA][CT]^CG
BsmBI        2801  CGTCTC               CGTCTC(1/5)
Bsp1407I     4078  TGTACA               T^GTACA
BspDI         271  ATCGAT               AT^CGAT
BspEI        3886  TCCGGA               T^CCGGA
BspHI        1151  TCATGA               T^CATGA
BsrGI        4078  TGTACA               T^GTACA
BssSI        2722  CTCGTG               CACGAG(-5/-1)
ClaI          271  ATCGAT               AT^CGAT
EcoRI        1193  GAATTC               G^AATTC
KspI         5545  CCGCGG               CCGC^GG
MroI         3886  TCCGGA               T^CCGGA
RcaI         1151  TCATGA               T^CATGA
SacII        5545  CCGCGG               CCGC^GG
ScaI         1628  AGTACT               AGT^ACT
SexAI          52  ACCTGGT              A^CC[AT]GGT
SspBI        4078  TGTACA               T^GTACA
XbaI         2287  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

Acc65I       1244  GGTACC               G^GTACC
             1811  GGTACC              
Aor51HI      1345  AGCGCT               AGC^GCT
             4449  AGCGCT              
Asp700I      1614  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3674  GAAGCCCTTC          
Asp718I      1244  GGTACC               G^GTACC
             1811  GGTACC              
AviII        1694  TGCGCA               TGC^GCA
             2345  TGCGCA              
BamHI        3889  GGATCC               G^GATCC
             5541  GGATCC              
BbeI          312  GGCGCC               GGCGC^C
             4176  GGCGCC              
BbsI         4301  GAAGAC               GAAGAC(2/6)
             5220  GAAGAC              
BbvCI         488  CCTCAGC              CCTCAGC(-5/-2)
             5102  GCTGAGG             
BciVI         665  GTATCC               GTATCC(6/5)
             5532  GTATCC              
BglI         3960  GCCACATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5254  GCCATCATGGC         
BlpI         2472  GCTGAGC              GC^T[ACGT]AGC
             3969  GCTGAGC             
Bpu1102I     2472  GCTGAGC              GC^T[ACGT]AGC
             3969  GCTGAGC             
BpuAI        4301  GAAGAC               GAAGAC(2/6)
             5220  GAAGAC              
BsrDI         179  GCAATG               GCAATG(2/0)
             1418  GCAATG              
BstAPI       1686  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5447  GCAGCCACTGC         
CelII        2472  GCTGAGC              GC^T[ACGT]AGC
             3969  GCTGAGC             
DrdI         2610  GACCCGGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5354  GACAGATAAGTC        
Eco47III     1345  AGCGCT               AGC^GCT
             4449  AGCGCT              
EcoNI        1083  CCTCCCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4646  CCTGGCGGAGG         
FspI         1694  TGCGCA               TGC^GCA
             2345  TGCGCA              
HincII         90  GTTGAC               GT[CT]^[GA]AC
             1033  GTCAAC              
HindII         90  GTTGAC               GT[CT]^[GA]AC
             1033  GTCAAC              
KasI          312  GGCGCC               G^GCGCC
             4176  GGCGCC              
KpnI         1244  GGTACC               GGTAC^C
             1811  GGTACC              
NaeI          187  GCCGGC               GCC^GGC
             4006  GCCGGC              
NarI          312  GGCGCC               GG^CGCC
             4176  GGCGCC              
NgoMIV        187  GCCGGC               G^CCGGC
             4006  GCCGGC              
NheI          291  GCTAGC               G^CTAGC
             5098  GCTAGC              
SbfI         2304  CCTGCAGG             CCTGCA^GG
             3764  CCTGCAGG            
SfoI          312  GGCGCC               GGC^GCC
             4176  GGCGCC              
SphI         2240  GCATGC               GCATG^C
             2675  GCATGC              
Sse8387I     2304  CCTGCAGG             CCTGCA^GG
             3764  CCTGCAGG            
XmnI         1614  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3674  GAAGCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbrPI       BbvI        BcgI        BcnI        
BfaI        BmyI        BpmI        BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI