##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AviII AvrII BbrPI BcgI
BglI BlnI BsaAI BsaBI BsiWI
BsmBI BsrBI BssHII Bst1107I BstEII
BstPI BstZ17I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI KspI MamI
MluI NheI NotI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SexAI SfiI SgrAI SmaI
SnaBI SpeI StuI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 3164 GGTACC G^GTACC
AccIII 115 TCCGGA T^CCGGA
AcyI 5032 GGCGCC G[GA]^CG[CT]C
AflII 2876 CTTAAG C^TTAAG
Asp718I 3164 GGTACC G^GTACC
BamHI 5321 GGATCC G^GATCC
BbeI 5032 GGCGCC GGCGC^C
BclI 2813 TGATCA T^GATCA
BfrI 2876 CTTAAG C^TTAAG
BlpI 322 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 322 GCTGAGC GC^T[ACGT]AGC
BsaHI 5032 GGCGCC G[GA]^CG[CT]C
BsaWI 115 TCCGGA [AT]^CCGG[AT]
BseAI 115 TCCGGA T^CCGGA
BsiEI 291 CGACCG CG[GA][CT]^CG
Bsp1407I 765 TGTACA T^GTACA
BspDI 5026 ATCGAT AT^CGAT
BspEI 115 TCCGGA T^CCGGA
BspHI 1257 TCATGA T^CATGA
BsrFI 318 GCCGGC [GA]^CCGG[CT]
BsrGI 765 TGTACA T^GTACA
BstAPI 5334 GCAATTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 169 TTCGAA TT^CGAA
BstXI 3644 CCATGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 322 GCTGAGC GC^T[ACGT]AGC
Cfr10I 318 GCCGGC [GA]^CCGG[CT]
ClaI 5026 ATCGAT AT^CGAT
EcoNI 1744 CCTAAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2813 TGATCA T^GATCA
HgaI 7 GCGTC GACGC(5/10)
Hin1I 5032 GGCGCC G[GA]^CG[CT]C
HindIII 1619 AAGCTT A^AGCTT
HpaI 70 GTTAAC GTT^AAC
KasI 5032 GGCGCC G^GCGCC
KpnI 3164 GGTACC GGTAC^C
MroI 115 TCCGGA T^CCGGA
NaeI 318 GCCGGC GCC^GGC
NarI 5032 GGCGCC GG^CGCC
NgoMIV 318 GCCGGC G^CCGGC
NruI 203 TCGCGA TCG^CGA
NspV 169 TTCGAA TT^CGAA
PstI 4537 CTGCAG CTGCA^G
PvuII 1205 CAGCTG CAG^CTG
RcaI 1257 TCATGA T^CATGA
SacI 261 GAGCTC GAGCT^C
SbfI 4536 CCTGCAGG CCTGCA^GG
SfoI 5032 GGCGCC GGC^GCC
SfuI 169 TTCGAA TT^CGAA
SphI 861 GCATGC GCATG^C
Sse8387I 4536 CCTGCAGG CCTGCA^GG
SspBI 765 TGTACA T^GTACA
SspI 1950 AATATT AAT^ATT
SwaI 131 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1503 ACATGT A^C[GA][CT]GT
4471 ACATGT
AocI 895 CCTGAGG CC^T[ACGT]AGG
1744 CCTAAGG
AspHI 261 GAGCTC G[AT]GC[AT]^C
4741 GAGCAC
AvaI 226 CCCGAG C^[CT]CG[GA]G
284 CTCGGG
BalI 4899 TGGCCA TGG^CCA
5075 TGGCCA
BciVI 1827 GTATCC GTATCC(6/5)
1842 GTATCC
BcnI 1459 CCGGG CC^[GC]GG
4622 CCGGG
BsiHKAI 261 GAGCTC G[AT]GC[AT]^C
4741 GAGCAC
BsmI 1785 GCATTC GAATGC(1/-1)
2826 GCATTC
BsoBI 226 CCCGAG C^[CT]CG[GA]G
284 CTCGGG
BspLU11I 1503 ACATGT A^CATGT
4471 ACATGT
BssSI 258 CACGAG CACGAG(-5/-1)
4876 CACGAG
Bsu36I 895 CCTGAGG CC^T[ACGT]AGG
1744 CCTAAGG
Eco81I 895 CCTGAGG CC^T[ACGT]AGG
1744 CCTAAGG
EcoRI 1180 GAATTC G^AATTC
4632 GAATTC
EcoRV 243 GATATC GAT^ATC
4308 GATATC
FauI 279 CCCGC CCCGC(4/6)
381 GCGGG
HaeII 1043 AGCGCC [GA]GCGC^[CT]
5032 GGCGCC
MfeI 3280 CAATTG C^AATTG
5249 CAATTG
MluNI 4899 TGGCCA TGG^CCA
5075 TGGCCA
MscI 4899 TGGCCA TGG^CCA
5075 TGGCCA
MspA1I 1205 CAGCTG C[AC]G^C[GT]G
1567 CAGCGG
MunI 3280 CAATTG C^AATTG
5249 CAATTG
NciI 1459 CCGGG CC^[GC]GG
4622 CCGGG
NdeI 2159 CATATG CA^TATG
4170 CATATG
PflMI 649 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4257 CCATATTCTGG
ScaI 359 AGTACT AGT^ACT
2246 AGTACT
Van91I 649 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4257 CCATATTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspI AvaII BanI BanII
BbsI BbvCI BbvI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflFI PleI PpuMI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XcmI XhoII
XmnI