##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AvrII BciVI BclI BfrI BglI
BglII BlnI BsaBI BsiWI BsmBI
BspDI BssHII Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraIII Eco47III
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI KspI MamI MluI NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SgrAI SnaBI SphI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 3119 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 497 CCTGAGG CC^T[ACGT]AGG
AspEI 3119 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1742 GGATCC G^GATCC
BbrPI 1804 CACGTG CAC^GTG
BsmI 547 GAATGC GAATGC(1/-1)
BsrFI 4471 GCCGGC [GA]^CCGG[CT]
BssSI 1309 CACGAG CACGAG(-5/-1)
BstBI 1119 TTCGAA TT^CGAA
Bsu36I 497 CCTGAGG CC^T[ACGT]AGG
Cfr10I 4471 GCCGGC [GA]^CCGG[CT]
DrdI 1439 GACACGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3119 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 497 CCTGAGG CC^T[ACGT]AGG
HpaI 4555 GTTAAC GTT^AAC
NaeI 4471 GCCGGC GCC^GGC
NdeI 5212 CATATG CA^TATG
NgoMIV 4471 GCCGGC G^CCGGC
NspV 1119 TTCGAA TT^CGAA
PmaCI 1804 CACGTG CAC^GTG
PmlI 1804 CACGTG CAC^GTG
SapI 3970 GAAGAGC GCTCTTC(1/4)
SfuI 1119 TTCGAA TT^CGAA
SmaI 2479 CCCGGG CCC^GGG
SpeI 4335 ACTAGT A^CTAGT
StuI 352 AGGCCT AGG^CCT
XmaCI 2479 CCCGGG C^CCGGG
XmaI 2479 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 2151 TCCGGA T^CCGGA
2430 TCCGGA
Asp700I 1867 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2056 GAAGGTCTTC
AspI 894 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4614 GACAGAGTC
AvaI 1246 CTCGGG C^[CT]CG[GA]G
2479 CCCGGG
AviII 579 TGCGCA TGC^GCA
723 TGCGCA
BbvCI 2539 CCTCAGC CCTCAGC(-5/-2)
4707 CCTCAGC
BsaI 976 GAGACC GGTCTC(1/5)
4809 GGTCTC
BsaWI 2151 TCCGGA [AT]^CCGG[AT]
2430 TCCGGA
BseAI 2151 TCCGGA T^CCGGA
2430 TCCGGA
BsoBI 1246 CTCGGG C^[CT]CG[GA]G
2479 CCCGGG
Bsp1407I 1149 TGTACA T^GTACA
4383 TGTACA
BspEI 2151 TCCGGA T^CCGGA
2430 TCCGGA
BspHI 816 TCATGA T^CATGA
2845 TCATGA
BspLU11I 261 ACATGT A^CATGT
3877 ACATGT
BsrBI 2410 CCGCTC CCGCTC(-3/-3)
1340 GAGCGG
BsrGI 1149 TGTACA T^GTACA
4383 TGTACA
BstYI 27 GGATCT [GA]^GATC[CT]
1742 GGATCC
EagI 2176 CGGCCG C^GGCCG
5125 CGGCCG
EclXI 2176 CGGCCG C^GGCCG
5125 CGGCCG
Eco52I 2176 CGGCCG C^GGCCG
5125 CGGCCG
EcoT22I 521 ATGCAT ATGCA^T
5156 ATGCAT
FspI 579 TGCGCA TGC^GCA
723 TGCGCA
HindIII 304 AAGCTT A^AGCTT
3452 AAGCTT
MfeI 1777 CAATTG C^AATTG
5286 CAATTG
MflI 27 GGATCT [GA]^GATC[CT]
1742 GGATCC
MroI 2151 TCCGGA T^CCGGA
2430 TCCGGA
MunI 1777 CAATTG C^AATTG
5286 CAATTG
NsiI 521 ATGCAT ATGCA^T
5156 ATGCAT
PflFI 894 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4614 GACAGAGTC
PpuMI 2103 AGGACCC [GA]G^G[AT]CC[CT]
2211 AGGTCCC
RcaI 816 TCATGA T^CATGA
2845 TCATGA
ScaI 1917 AGTACT AGT^ACT
3068 AGTACT
SspBI 1149 TGTACA T^GTACA
4383 TGTACA
SspI 3776 AATATT AAT^ATT
4082 AATATT
TaqI 801 TCGA T^CGA
1120 TCGA
Tth111I 894 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4614 GACAGAGTC
TthHB8I 801 TCGA T^CGA
1120 TCGA
XhoII 27 GGATCT [GA]^GATC[CT]
1742 GGATCC
XmnI 1867 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2056 GAAGGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI ApaI ApoI Asp718I AspHI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcgI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI