##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AflII AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI BamHI BbeI BbrPI
BcgI BciVI BfrI BglI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FseI
HincII HindII HpaI KasI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacII SalI SapI SfiI SfoI
SfuI SgrAI SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AclI 582 AACGTT AA^CGTT
AflIII 3802 ACGTGT A^C[GA][CT]GT
AgeI 1201 ACCGGT A^CCGGT
AspHI 5268 GAGCTC G[AT]GC[AT]^C
AvrII 4998 CCTAGG C^CTAGG
BglII 2866 AGATCT A^GATCT
BlnI 4998 CCTAGG C^CTAGG
BsaAI 5093 TACGTA [CT]AC^GT[GA]
BsaBI 1292 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 5268 GAGCTC G[AT]GC[AT]^C
BspHI 3751 TCATGA T^CATGA
BsrFI 1201 ACCGGT [GA]^CCGG[CT]
BssHII 20 GCGCGC G^CGCGC
Cfr10I 1201 ACCGGT [GA]^CCGG[CT]
DraIII 4802 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 4129 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4824 GAATTC G^AATTC
EcoRV 363 GATATC GAT^ATC
MamI 1292 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2111 CATATG CA^TATG
PaeR7I 4554 CTCGAG C^TCGAG
PinAI 1201 ACCGGT A^CCGGT
Psp1406I 582 AACGTT AA^CGTT
RcaI 3751 TCATGA T^CATGA
SacI 5268 GAGCTC GAGCT^C
ScaI 1456 AGTACT AGT^ACT
SnaBI 5093 TACGTA TAC^GTA
SphI 478 GCATGC GCATG^C
StuI 5216 AGGCCT AGG^CCT
XhoI 4554 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 394 GGTACC G^GTACC
3715 GGTACC
AcyI 989 GACGCC G[GA]^CG[CT]C
3130 GGCGTC
AlwNI 879 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1404 CAGGTGCTG
ApaI 109 GGGCCC GGGCC^C
1450 GGGCCC
Asp718I 394 GGTACC G^GTACC
3715 GGTACC
AviII 940 TGCGCA TGC^GCA
1816 TGCGCA
BbvCI 318 CCTCAGC CCTCAGC(-5/-2)
324 CCTCAGC
BsaHI 989 GACGCC G[GA]^CG[CT]C
3130 GGCGTC
BsaI 5475 GAGACC GGTCTC(1/5)
2452 GGTCTC
BsaWI 1201 ACCGGT [AT]^CCGG[AT]
3707 TCCGGT
BsmBI 987 GAGACG CGTCTC(1/5)
1256 GAGACG
BstAPI 860 GCATAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4065 GCATTAAATGC
FspI 940 TGCGCA TGC^GCA
1816 TGCGCA
HaeII 53 GGCGCT [GA]GCGC^[CT]
1487 GGCGCT
Hin1I 989 GACGCC G[GA]^CG[CT]C
3130 GGCGTC
HindIII 809 AAGCTT A^AGCTT
4107 AAGCTT
KpnI 394 GGTACC GGTAC^C
3715 GGTACC
NcoI 412 CCATGG C^CATGG
1345 CCATGG
SbfI 2700 CCTGCAGG CCTGCA^GG
4412 CCTGCAGG
SexAI 4387 ACCTGGT A^CC[AT]GGT
4803 ACCAGGT
SmaI 736 CCCGGG CCC^GGG
1163 CCCGGG
Sse8387I 2700 CCTGCAGG CCTGCA^GG
4412 CCTGCAGG
XmaCI 736 CCCGGG C^CCGGG
1163 CCCGGG
XmaI 736 CCCGGG C^CCGGG
1163 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmnI