##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AccI AccIII AclI AflII AscI
BamHI BciVI BclI BfrI BglII
BlpI Bpu1102I BsaBI BseAI BsiWI
BspEI BspHI BspLU11I Bst1107I BstBI
BstZ17I CelII EcoRI FbaI FseI
HindIII HpaI MamI MluI MroI
NaeI NdeI NgoMIV NotI NspV
PacI PaeR7I PmeI PshAI Psp1406I
RcaI SalI SbfI ScaI SfuI
SgrAI SnaBI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 4785 GGTACC G^GTACC
AgeI 1280 ACCGGT A^CCGGT
AocI 2789 CCTCAGG CC^T[ACGT]AGG
AseI 3437 ATTAAT AT^TAAT
AsnI 3437 ATTAAT AT^TAAT
Asp718I 4785 GGTACC G^GTACC
AviII 4136 TGCGCA TGC^GCA
BbrPI 4932 CACGTG CAC^GTG
BbvCI 2672 CCTCAGC CCTCAGC(-5/-2)
BcgI 1257 CGACGCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BspDI 328 ATCGAT AT^CGAT
BsrBI 414 GAGCGG CCGCTC(-3/-3)
BsrDI 5571 CATTGC GCAATG(2/0)
BssHII 230 GCGCGC G^CGCGC
Bsu36I 2789 CCTCAGG CC^T[ACGT]AGG
ClaI 328 ATCGAT AT^CGAT
CpoI 1199 CGGACCG CG^G[AT]CCG
DraIII 5280 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1728 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2789 CCTCAGG CC^T[ACGT]AGG
EcoNI 1725 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4098 GATATC GAT^ATC
EcoT22I 1464 ATGCAT ATGCA^T
FspI 4136 TGCGCA TGC^GCA
KpnI 4785 GGTACC GGTAC^C
MfeI 4034 CAATTG C^AATTG
MunI 4034 CAATTG C^AATTG
NheI 684 GCTAGC G^CTAGC
NruI 5483 TCGCGA TCG^CGA
NsiI 1464 ATGCAT ATGCA^T
PflMI 3258 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1280 ACCGGT A^CCGGT
PmaCI 4932 CACGTG CAC^GTG
PmlI 4932 CACGTG CAC^GTG
PshBI 3437 ATTAAT AT^TAAT
PvuI 24 CGATCG CGAT^CG
RsrII 1199 CGGACCG CG^G[AT]CCG
SapI 3506 GAAGAGC GCTCTTC(1/4)
SexAI 755 ACCTGGT A^CC[AT]GGT
SfiI 1005 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1879 ACTAGT A^CTAGT
SphI 3489 GCATGC GCATG^C
SspI 1982 AATATT AAT^ATT
Van91I 3258 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1051 GACGTC GACGT^C
3552 GACGTC
AcsI 2013 GAATTT [GA]^AATT[CT]
4216 AAATTC
AflIII 4933 ACGTGT A^C[GA][CT]GT
5387 ACGTGT
AhdI 1279 GACCGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3891 GACCTGTCGTC
Alw44I 4929 GTGCAC G^TGCAC
5277 GTGCAC
Aor51HI 731 AGCGCT AGC^GCT
870 AGCGCT
ApaLI 4929 GTGCAC G^TGCAC
5277 GTGCAC
ApoI 2013 GAATTT [GA]^AATT[CT]
4216 AAATTC
AspEI 1279 GACCGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3891 GACCTGTCGTC
AvrII 3885 CCTAGG C^CTAGG
3910 CCTAGG
BbeI 115 GGCGCC GGCGC^C
920 GGCGCC
BlnI 3885 CCTAGG C^CTAGG
3910 CCTAGG
BsmBI 1087 GAGACG CGTCTC(1/5)
2106 GAGACG
BsmI 1462 GAATGC GAATGC(1/-1)
1065 GCATTC
BstEII 360 GGTGACC G^GT[ACGT]ACC
3528 GGTAACC
BstPI 360 GGTGACC G^GT[ACGT]ACC
3528 GGTAACC
DraI 5543 TTTAAA TTT^AAA
5562 TTTAAA
EagI 225 CGGCCG C^GGCCG
1589 CGGCCG
Eam1105I 1279 GACCGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3891 GACCTGTCGTC
EclXI 225 CGGCCG C^GGCCG
1589 CGGCCG
Eco47III 731 AGCGCT AGC^GCT
870 AGCGCT
Eco52I 225 CGGCCG C^GGCCG
1589 CGGCCG
EcoO65I 360 GGTGACC G^GT[ACGT]ACC
3528 GGTAACC
KasI 115 GGCGCC G^GCGCC
920 GGCGCC
NarI 115 GGCGCC GG^CGCC
920 GGCGCC
NcoI 1016 CCATGG C^CATGG
3124 CCATGG
NspI 2277 GCATGT [GA]CATG^[CT]
3489 GCATGC
SfoI 115 GGCGCC GGC^GCC
920 GGCGCC
StuI 2524 AGGCCT AGG^CCT
3189 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII XmaCI XmaI XmnI