##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AflII AgeI AscI
AseI AsnI Asp718I AspI AvrII
BamHI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsiWI BspDI
BspLU11I BssHII Bst1107I BstZ17I ClaI
CpoI DraI EcoRI EcoRV EcoT22I
FbaI HindIII KpnI MamI MfeI
MunI NdeI NheI NotI NruI
NsiI PacI PflFI PinAI PmeI
PshBI PvuI RsrII SalI SexAI
SgrAI SnaBI SpeI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3061 GTCTAC GT^[AC][GT]AC
AclI 3597 AACGTT AA^CGTT
AhdI 4347 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4913 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1282 AGCGCT AGC^GCT
Asp700I 2268 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4347 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 5344 CACGTG CAC^GTG
BbvCI 1761 CCTCAGC CCTCAGC(-5/-2)
BlpI 4922 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4922 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 3533 TGTACA T^GTACA
BsrDI 1161 CATTGC GCAATG(2/0)
BsrGI 3533 TGTACA T^GTACA
BstBI 527 TTCGAA TT^CGAA
BstEII 2811 GGTGACC G^GT[ACGT]ACC
BstPI 2811 GGTGACC G^GT[ACGT]ACC
Bsu36I 4913 CCTCAGG CC^T[ACGT]AGG
CelII 4922 GCTGAGC GC^T[ACGT]AGC
DrdI 2994 GACGAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4347 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1282 AGCGCT AGC^GCT
Eco81I 4913 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2811 GGTGACC G^GT[ACGT]ACC
FseI 2197 GGCCGGCC GGCCGG^CC
HpaI 5390 GTTAAC GTT^AAC
KspI 3748 CCGCGG CCGC^GG
MluI 5213 ACGCGT A^CGCGT
NspV 527 TTCGAA TT^CGAA
PaeR7I 5399 CTCGAG C^TCGAG
PmaCI 5344 CACGTG CAC^GTG
PmlI 5344 CACGTG CAC^GTG
Psp1406I 3597 AACGTT AA^CGTT
SacII 3748 CCGCGG CCGC^GG
SbfI 795 CCTGCAGG CCTGCA^GG
SfiI 3508 GGCCCACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 527 TTCGAA TT^CGAA
SphI 323 GCATGC GCATG^C
Sse8387I 795 CCTGCAGG CCTGCA^GG
SspBI 3533 TGTACA T^GTACA
SspI 2535 AATATT AAT^ATT
XhoI 5399 CTCGAG C^TCGAG
XmnI 2268 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AflIII 4468 ACACGT A^C[GA][CT]GT
5213 ACGCGT
AviII 1336 TGCGCA TGC^GCA
3194 TGCGCA
BbeI 3391 GGCGCC GGCGC^C
3758 GGCGCC
FspI 1336 TGCGCA TGC^GCA
3194 TGCGCA
KasI 3391 GGCGCC G^GCGCC
3758 GGCGCC
NarI 3391 GGCGCC GG^CGCC
3758 GGCGCC
PshAI 646 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
859 GACCTGGGTC
SfoI 3391 GGCGCC GGC^GCC
3758 GGCGCC
SmlI 4544 CTTGAG C^T[CT][GA]AG
5399 CTCGAG
StuI 80 AGGCCT AGG^CCT
3134 AGGCCT
XcmI 1781 CCAGCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3395 CCATCAGCTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI