##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AclI AgeI AhdI Aor51HI AscI
AspEI AviII AvrII BbeI BbvCI
BcgI BlnI BsiWI BspDI BssHII
Bst1107I BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI HindIII HpaI KasI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RsrII SalI SfiI SfoI SgrAI
SnaBI SphI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1415 GACGTC GACGT^C
AccIII 3053 TCCGGA T^CCGGA
AflII 808 CTTAAG C^TTAAG
ApaI 565 GGGCCC GGGCC^C
AspI 1974 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1461 TGGCCA TGG^CCA
BamHI 4118 GGATCC G^GATCC
BfrI 808 CTTAAG C^TTAAG
BglI 5263 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 588 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1972 GAGACC GGTCTC(1/5)
BseAI 3053 TCCGGA T^CCGGA
BsiEI 2692 CGACCG CG[GA][CT]^CG
BsmBI 873 GAGACG CGTCTC(1/5)
BspEI 3053 TCCGGA T^CCGGA
BsrBI 354 CCGCTC CCGCTC(-3/-3)
BssSI 1591 CACGAG CACGAG(-5/-1)
BstBI 3760 TTCGAA TT^CGAA
BstEII 1612 GGTTACC G^GT[ACGT]ACC
BstPI 1612 GGTTACC G^GT[ACGT]ACC
EcoO65I 1612 GGTTACC G^GT[ACGT]ACC
HaeII 2509 GGCGCT [GA]GCGC^[CT]
KspI 3145 CCGCGG CCGC^GG
MamI 588 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1461 TGGCCA TGG^CCA
MroI 3053 TCCGGA T^CCGGA
MscI 1461 TGGCCA TGG^CCA
NruI 3122 TCGCGA TCG^CGA
NspV 3760 TTCGAA TT^CGAA
PflFI 1974 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 3145 CCGCGG CCGC^GG
SbfI 1807 CCTGCAGG CCTGCA^GG
ScaI 4882 AGTACT AGT^ACT
SexAI 335 ACCTGGT A^CC[AT]GGT
SfuI 3760 TTCGAA TT^CGAA
SpeI 6367 ACTAGT A^CTAGT
Sse8387I 1807 CCTGCAGG CCTGCA^GG
Tth111I 1974 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 221 GGCGTC G[GA]^CG[CT]C
1415 GACGTC
Alw44I 2286 GTGCAC G^TGCAC
5409 GTGCAC
AocI 2077 CCTGAGG CC^T[ACGT]AGG
2806 CCTGAGG
ApaLI 2286 GTGCAC G^TGCAC
5409 GTGCAC
AseI 5625 ATTAAT AT^TAAT
6315 ATTAAT
AsnI 5625 ATTAAT AT^TAAT
6315 ATTAAT
Asp700I 1774 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6396 GAAGGTATTC
BlpI 1077 GCTCAGC GC^T[ACGT]AGC
3597 GCTGAGC
Bpu1102I 1077 GCTCAGC GC^T[ACGT]AGC
3597 GCTGAGC
BsaHI 221 GGCGTC G[GA]^CG[CT]C
1415 GACGTC
BsaWI 582 ACCGGA [AT]^CCGG[AT]
3053 TCCGGA
Bsp1407I 4437 TGTACA T^GTACA
6154 TGTACA
BspHI 1161 TCATGA T^CATGA
6897 TCATGA
BspLU11I 3116 ACATGT A^CATGT
4798 ACATGT
BsrDI 6545 CATTGC GCAATG(2/0)
2117 GCAATG
BsrFI 2582 GCCGGT [GA]^CCGG[CT]
2762 ACCGGC
BsrGI 4437 TGTACA T^GTACA
6154 TGTACA
Bsu36I 2077 CCTGAGG CC^T[ACGT]AGG
2806 CCTGAGG
CelII 1077 GCTCAGC GC^T[ACGT]AGC
3597 GCTGAGC
Cfr10I 2582 GCCGGT [GA]^CCGG[CT]
2762 ACCGGC
DraIII 2154 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
2679 CACGACGTG
Eco81I 2077 CCTGAGG CC^T[ACGT]AGG
2806 CCTGAGG
EcoNI 1405 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2260 CCTGCCGAAGG
EcoRV 2768 GATATC GAT^ATC
4273 GATATC
Hin1I 221 GGCGTC G[GA]^CG[CT]C
1415 GACGTC
NcoI 1502 CCATGG C^CATGG
6586 CCATGG
PpuMI 1396 AGGACCT [GA]G^G[AT]CC[CT]
6708 GGGACCC
PshBI 5625 ATTAAT AT^TAAT
6315 ATTAAT
RcaI 1161 TCATGA T^CATGA
6897 TCATGA
SmaI 197 CCCGGG CCC^GGG
3731 CCCGGG
SspBI 4437 TGTACA T^GTACA
6154 TGTACA
XbaI 3967 TCTAGA T^CTAGA
4954 TCTAGA
XmaCI 197 CCCGGG C^CCGGG
3731 CCCGGG
XmaI 197 CCCGGG C^CCGGG
3731 CCCGGG
XmnI 1774 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6396 GAAGGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp718I AspHI AvaI AvaII
BanI BanII BbrPI BbsI BbvI
BciVI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PmaCI
PmlI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII