##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AccIII      Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        BamHI       BbrPI       
BcgI        BsaBI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoRV       FseI        HpaI        
MamI        MluI        MroI        NaeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RsrII       SalI        SbfI        SfiI        
SfuI        SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        4682  GACGTC               GACGT^C
Acc65I       5185  GGTACC               G^GTACC
AclI         1491  AACGTT               AA^CGTT
AflIII       5534  ACATGT               A^C[GA][CT]GT
AgeI          990  ACCGGT               A^CCGGT
ApaI         2366  GGGCCC               GGGCC^C
Asp718I      5185  GGTACC               G^GTACC
AviII         100  TGCGCA               TGC^GCA
BalI          775  TGGCCA               TGG^CCA
BciVI        5915  GTATCC               GTATCC(6/5)
BglI         1535  GCCGCCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        5923  AGATCT               A^GATCT
BsaAI        3881  CACGTA               [CT]AC^GT[GA]
BsiEI         988  CGACCG               CG[GA][CT]^CG
BsmBI         149  GAGACG               CGTCTC(1/5)
BspLU11I     5534  ACATGT               A^CATGT
BspMI        4349  GCAGGT               ACCTGC(4/8)
BstEII        461  GGTGACC              G^GT[ACGT]ACC
BstPI         461  GGTGACC              G^GT[ACGT]ACC
BstXI        5454  CCAACACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         5685  TTTAAA               TTT^AAA
EcoO65I       461  GGTGACC              G^GT[ACGT]ACC
EcoRI        2744  GAATTC               G^AATTC
FspI          100  TGCGCA               TGC^GCA
KpnI         5185  GGTACC               GGTAC^C
KspI          554  CCGCGG               CCGC^GG
MluNI         775  TGGCCA               TGG^CCA
MscI          775  TGGCCA               TGG^CCA
NcoI         3217  CCATGG               C^CATGG
PaeR7I       2874  CTCGAG               C^TCGAG
PinAI         990  ACCGGT               A^CCGGT
Psp1406I     1491  AACGTT               AA^CGTT
SacII         554  CCGCGG               CCGC^GG
SexAI        5193  ACCTGGT              A^CC[AT]GGT
SgrAI         881  CACCGGCG             C[GA]^CCGG[CT]G
SspI         5569  AATATT               AAT^ATT
XhoI         2874  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         2285  GTCTAC               GT^[AC][GT]AC
             2395  GTCTAC              
AflII        4928  CTTAAG               C^TTAAG
             5821  CTTAAG              
AvaI           92  CCCGAG               C^[CT]CG[GA]G
             2874  CTCGAG              
AvrII        4781  CCTAGG               C^CTAGG
             5896  CCTAGG              
BclI         4572  TGATCA               T^GATCA
             5132  TGATCA              
BfrI         4928  CTTAAG               C^TTAAG
             5821  CTTAAG              
BlnI         4781  CCTAGG               C^CTAGG
             5896  CCTAGG              
BsaWI         477  ACCGGA               [AT]^CCGG[AT]
              990  ACCGGT              
BsoBI          92  CCCGAG               C^[CT]CG[GA]G
             2874  CTCGAG              
BspHI        2953  TCATGA               T^CATGA
             5092  TCATGA              
BsrDI        1328  GCAATG               GCAATG(2/0)
             2820  GCAATG              
BsrFI         882  ACCGGC               [GA]^CCGG[CT]
              990  ACCGGT              
BssSI         507  CACGAG               CACGAG(-5/-1)
             2787  CTCGTG              
Cfr10I        882  ACCGGC               [GA]^CCGG[CT]
              990  ACCGGT              
DraIII        914  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1445  CACAGTGTG           
EcoT22I      1089  ATGCAT               ATGCA^T
             1400  ATGCAT              
FbaI         4572  TGATCA               T^GATCA
             5132  TGATCA              
HgaI          790  GACGC                GACGC(5/10)
             2005  GCGTC               
HincII       3386  GTTGAC               GT[CT]^[GA]AC
             3857  GTCAAC              
HindII       3386  GTTGAC               GT[CT]^[GA]AC
             3857  GTCAAC              
MfeI         1314  CAATTG               C^AATTG
             3231  CAATTG              
MunI         1314  CAATTG               C^AATTG
             3231  CAATTG              
NsiI         1089  ATGCAT               ATGCA^T
             1400  ATGCAT              
NspI         4959  GCATGT               [GA]CATG^[CT]
             5534  ACATGT              
PpuMI        3917  GGGTCCT              [GA]G^G[AT]CC[CT]
             5848  GGGTCCC             
PvuII        3464  CAGCTG               CAG^CTG
             4077  CAGCTG              
RcaI         2953  TCATGA               T^CATGA
             5092  TCATGA              
SacI          236  GAGCTC               GAGCT^C
             2078  GAGCTC              
ScaI         4668  AGTACT               AGT^ACT
             5417  AGTACT              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AhdI        AluI        AlwI        AlwNI       ApoI        
Asp700I     AspEI       AspHI       AspI        AvaII       
BanI        BanII       BbeI        BbsI        BbvCI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eam1105I    EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NdeI        
NdeII       NlaIII      NlaIV       PflFI       PflMI       
PleI        PstI        RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmnI