##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
Aor51HI AscI AspI BbeI BcgI
BciVI BglII BsaWI BseAI BsiWI
BspDI BspEI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eco47III
EcoRV FseI KasI KspI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
RsrII SacII SalI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 4005 GGTACC G^GTACC
AcyI 5773 GGCGTC G[GA]^CG[CT]C
AflII 5082 CTTAAG C^TTAAG
AhdI 2305 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5457 GTGCAC G^TGCAC
AocI 5716 CCTCAGG CC^T[ACGT]AGG
ApaLI 5457 GTGCAC G^TGCAC
AseI 5484 ATTAAT AT^TAAT
AsnI 5484 ATTAAT AT^TAAT
Asp700I 4341 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4005 GGTACC G^GTACC
AspEI 2305 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 93 CTCGGG C^[CT]CG[GA]G
AvrII 2635 CCTAGG C^CTAGG
BfrI 5082 CTTAAG C^TTAAG
BlnI 2635 CCTAGG C^CTAGG
BsaHI 5773 GGCGTC G[GA]^CG[CT]C
BsmBI 4504 GAGACG CGTCTC(1/5)
BsmI 2320 GAATGC GAATGC(1/-1)
BsoBI 93 CTCGGG C^[CT]CG[GA]G
BspLU11I 5881 ACATGT A^CATGT
BsrFI 132 GCCGGC [GA]^CCGG[CT]
BstAPI 78 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1614 GGTCACC G^GT[ACGT]ACC
BstPI 1614 GGTCACC G^GT[ACGT]ACC
Bsu36I 5716 CCTCAGG CC^T[ACGT]AGG
Cfr10I 132 GCCGGC [GA]^CCGG[CT]
DrdI 3491 GACCATCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2305 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5716 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1614 GGTCACC G^GT[ACGT]ACC
EcoRI 1022 GAATTC G^AATTC
EcoT22I 925 ATGCAT ATGCA^T
Hin1I 5773 GGCGTC G[GA]^CG[CT]C
HindIII 1806 AAGCTT A^AGCTT
HpaI 247 GTTAAC GTT^AAC
KpnI 4005 GGTACC GGTAC^C
NaeI 132 GCCGGC GCC^GGC
NcoI 2883 CCATGG C^CATGG
NgoMIV 132 GCCGGC G^CCGGC
NsiI 925 ATGCAT ATGCA^T
PshBI 5484 ATTAAT AT^TAAT
PvuI 4470 CGATCG CGAT^CG
SfiI 58 GGCCTTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XbaI 5306 TCTAGA T^CTAGA
XmnI 4341 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 20 CGCG CG^CG
143 CGCG
AviII 155 TGCGCA TGC^GCA
3061 TGCGCA
BanI 4005 GGTACC G^G[CT][GA]CC
4817 GGTGCC
BbrPI 3289 CACGTG CAC^GTG
3458 CACGTG
BclI 276 TGATCA T^GATCA
1511 TGATCA
BglI 59 GCCTTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3838 GCCAGATTGGC
BsaAI 3289 CACGTG [CT]AC^GT[GA]
3458 CACGTG
Bsp1407I 2752 TGTACA T^GTACA
5878 TGTACA
BspHI 1841 TCATGA T^CATGA
3201 TCATGA
BsrBI 4009 CCGCTC CCGCTC(-3/-3)
1449 GAGCGG
BsrDI 365 CATTGC GCAATG(2/0)
1812 CATTGC
BsrGI 2752 TGTACA T^GTACA
5878 TGTACA
BstUI 20 CGCG CG^CG
143 CGCG
DraIII 1741 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3740 CACCCTGTG
EagI 66 CGGCCG C^GGCCG
130 CGGCCG
EclXI 66 CGGCCG C^GGCCG
130 CGGCCG
Eco52I 66 CGGCCG C^GGCCG
130 CGGCCG
FbaI 276 TGATCA T^GATCA
1511 TGATCA
FspI 155 TGCGCA TGC^GCA
3061 TGCGCA
HaeII 3226 GGCGCT [GA]GCGC^[CT]
4695 GGCGCT
MvnI 20 CGCG CG^CG
143 CGCG
PflMI 4166 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5345 CCAATACATGG
PmaCI 3289 CACGTG CAC^GTG
3458 CACGTG
PmlI 3289 CACGTG CAC^GTG
3458 CACGTG
RcaI 1841 TCATGA T^CATGA
3201 TCATGA
ScaI 487 AGTACT AGT^ACT
3579 AGTACT
SspBI 2752 TGTACA T^GTACA
5878 TGTACA
SspI 5301 AATATT AAT^ATT
5913 AATATT
Van91I 4166 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5345 CCAATACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BalI BamHI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NheI NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII