##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
AspEI BciVI BfrI BsaBI BsiWI
BspDI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI Eam1105I EcoO65I
EcoRI EcoRV FseI HpaI KspI
MamI MfeI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 2061 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1782 CCTAGG C^CTAGG
BamHI 3856 GGATCC G^GATCC
BclI 3448 TGATCA T^GATCA
BglII 1076 AGATCT A^GATCT
BlnI 1782 CCTAGG C^CTAGG
BsmI 2288 GCATTC GAATGC(1/-1)
Bsp1407I 2072 TGTACA T^GTACA
BspLU11I 1251 ACATGT A^CATGT
BsrGI 2072 TGTACA T^GTACA
BssHII 1445 GCGCGC G^CGCGC
FbaI 3448 TGATCA T^GATCA
PflMI 2988 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 627 GAGCTC GAGCT^C
SapI 907 GAAGAGC GCTCTTC(1/4)
SphI 5484 GCATGC GCATG^C
SspBI 2072 TGTACA T^GTACA
Van91I 2988 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2061 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 2423 TCCGGA T^CCGGA
4230 TCCGGA
Alw44I 3172 GTGCAC G^TGCAC
4537 GTGCAC
Aor51HI 698 AGCGCT AGC^GCT
2670 AGCGCT
ApaLI 3172 GTGCAC G^TGCAC
4537 GTGCAC
BbeI 4890 GGCGCC GGCGC^C
5821 GGCGCC
BbrPI 2365 CACGTG CAC^GTG
3776 CACGTG
BsaAI 2365 CACGTG [CT]AC^GT[GA]
3776 CACGTG
BseAI 2423 TCCGGA T^CCGGA
4230 TCCGGA
BsiEI 244 CGGCCG CG[GA][CT]^CG
3559 CGGCCG
BspEI 2423 TCCGGA T^CCGGA
4230 TCCGGA
BsrDI 1211 GCAATG GCAATG(2/0)
4483 GCAATG
DrdI 751 GACCCCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4106 GACAACCTTGTC
EagI 244 CGGCCG C^GGCCG
3559 CGGCCG
EclXI 244 CGGCCG C^GGCCG
3559 CGGCCG
Eco47III 698 AGCGCT AGC^GCT
2670 AGCGCT
Eco52I 244 CGGCCG C^GGCCG
3559 CGGCCG
EcoT22I 2079 ATGCAT ATGCA^T
5486 ATGCAT
HincII 4574 GTTGAC GT[CT]^[GA]AC
4933 GTTGAC
HindII 4574 GTTGAC GT[CT]^[GA]AC
4933 GTTGAC
HindIII 3334 AAGCTT A^AGCTT
3525 AAGCTT
KasI 4890 GGCGCC G^GCGCC
5821 GGCGCC
MluI 590 ACGCGT A^CGCGT
1991 ACGCGT
MroI 2423 TCCGGA T^CCGGA
4230 TCCGGA
NaeI 3557 GCCGGC GCC^GGC
4927 GCCGGC
NarI 4890 GGCGCC GG^CGCC
5821 GGCGCC
NgoMIV 3557 GCCGGC G^CCGGC
4927 GCCGGC
NsiI 2079 ATGCAT ATGCA^T
5486 ATGCAT
PaeR7I 1000 CTCGAG C^TCGAG
2224 CTCGAG
PmaCI 2365 CACGTG CAC^GTG
3776 CACGTG
PmlI 2365 CACGTG CAC^GTG
3776 CACGTG
SbfI 1236 CCTGCAGG CCTGCA^GG
5038 CCTGCAGG
SexAI 431 ACCTGGT A^CC[AT]GGT
1541 ACCTGGT
SfoI 4890 GGCGCC GGC^GCC
5821 GGCGCC
Sse8387I 1236 CCTGCAGG CCTGCA^GG
5038 CCTGCAGG
StuI 2847 AGGCCT AGG^CCT
4225 AGGCCT
TfiI 936 GATTC G^A[AT]TC
5518 GAATC
XhoI 1000 CTCGAG C^TCGAG
2224 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI Asp718I AspHI AspI
AvaI AvaII AviII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmaCI XmaI