##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AclI AocI Aor51HI Asp718I
BamHI BglII BsiWI BsmBI BspDI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoT22I
FseI HincII HindII HpaI KpnI
MfeI MluI MunI NheI NotI
NruI NsiI NspV PacI PmeI
Psp1406I PvuI RsrII SalI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SwaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 4676 GACGTC GACGT^C
AflII 5774 CTTAAG C^TTAAG
AgeI 6445 ACCGGT A^CCGGT
AhdI 1010 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 154 GGCGCGCC GG^CGCGCC
AseI 6616 ATTAAT AT^TAAT
AsnI 6616 ATTAAT AT^TAAT
AspEI 1010 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 833 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 6156 GGATAC GTATCC(6/5)
BfrI 5774 CTTAAG C^TTAAG
BsiEI 6447 CGGTCG CG[GA][CT]^CG
BsmI 5801 GCATTC GAATGC(1/-1)
BspLU11I 2838 ACATGT A^CATGT
Eam1105I 1010 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4969 GATATC GAT^ATC
KspI 16 CCGCGG CCGC^GG
PflFI 833 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 6445 ACCGGT A^CCGGT
PshAI 3855 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6616 ATTAAT AT^TAAT
SacII 16 CCGCGG CCGC^GG
SexAI 4443 ACCTGGT A^CC[AT]GGT
SmaI 2715 CCCGGG CCC^GGG
Tth111I 833 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3847 TCTAGA T^CTAGA
XmaCI 2715 CCCGGG C^CCGGG
XmaI 2715 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 5539 GTAGAC GT^[AC][GT]AC
6247 GTAGAC
AccIII 739 TCCGGA T^CCGGA
4189 TCCGGA
AflIII 2838 ACATGT A^C[GA][CT]GT
3414 ACGTGT
Asp700I 2681 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4303 GAAGCCATTC
AviII 4689 TGCGCA TGC^GCA
4866 TGCGCA
AvrII 72 CCTAGG C^CTAGG
5766 CCTAGG
BbrPI 3413 CACGTG CAC^GTG
4994 CACGTG
BcgI 2704 CGAGAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
430 GCAGCTTCATCG
BclI 5508 TGATCA T^GATCA
6240 TGATCA
BlnI 72 CCTAGG C^CTAGG
5766 CCTAGG
BsaBI 1916 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4096 GATCGTAATC
BseAI 739 TCCGGA T^CCGGA
4189 TCCGGA
Bsp1407I 333 TGTACA T^GTACA
4656 TGTACA
BspEI 739 TCCGGA T^CCGGA
4189 TCCGGA
BspHI 1101 TCATGA T^CATGA
5686 TCATGA
BsrGI 333 TGTACA T^GTACA
4656 TGTACA
BssSI 1079 CTCGTG CACGAG(-5/-1)
5140 CTCGTG
BstEII 2720 GGTTACC G^GT[ACGT]ACC
4021 GGTCACC
BstPI 2720 GGTTACC G^GT[ACGT]ACC
4021 GGTCACC
EcoO65I 2720 GGTTACC G^GT[ACGT]ACC
4021 GGTCACC
FbaI 5508 TGATCA T^GATCA
6240 TGATCA
FspI 4689 TGCGCA TGC^GCA
4866 TGCGCA
MamI 1916 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4096 GATCGTAATC
MroI 739 TCCGGA T^CCGGA
4189 TCCGGA
NcoI 8 CCATGG C^CATGG
4695 CCATGG
NdeI 3573 CATATG CA^TATG
5644 CATATG
PmaCI 3413 CACGTG CAC^GTG
4994 CACGTG
PmlI 3413 CACGTG CAC^GTG
4994 CACGTG
RcaI 1101 TCATGA T^CATGA
5686 TCATGA
SbfI 964 CCTGCAGG CCTGCA^GG
1942 CCTGCAGG
SphI 1237 GCATGC GCATG^C
5022 GCATGC
Sse8387I 964 CCTGCAGG CCTGCA^GG
1942 CCTGCAGG
SspBI 333 TGTACA T^GTACA
4656 TGTACA
SspI 1366 AATATT AAT^ATT
5503 AATATT
XmnI 2681 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4303 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII