##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AocI        ApoI        AseI        AsnI        
Asp718I     BclI        BfrI        BglII       BsaBI       
BsiWI       BspDI       BspLU11I    BstBI       BstEII      
BstPI       Bsu36I      ClaI        CpoI        DrdI        
Eco81I      EcoO65I     EcoRI       EcoRV       FbaI        
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MunI        NdeI        NotI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SalI        SapI        
SbfI        SfiI        SfuI        SgrAI       SpeI        
Sse8387I    SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AflIII       5057  ACGTGT               A^C[GA][CT]GT
AhdI         5711  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2000  GTGCAC               G^TGCAC
Aor51HI      2902  AGCGCT               AGC^GCT
ApaLI        2000  GTGCAC               G^TGCAC
AscI         1225  GGCGCGCC             GG^CGCGCC
Asp700I      5622  GAACGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        5711  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        4149  TGCGCA               TGC^GCA
AvrII        5042  CCTAGG               C^CTAGG
BamHI        5132  GGATCC               G^GATCC
BbsI         3038  GTCTTC               GAAGAC(2/6)
BcgI          684  GCAGCGGAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3192  GTATCC               GTATCC(6/5)
BlnI         5042  CCTAGG               C^CTAGG
BpuAI        3038  GTCTTC               GAAGAC(2/6)
BsmBI        4751  GAGACG               CGTCTC(1/5)
Bsp1407I     1851  TGTACA               T^GTACA
BspHI        1380  TCATGA               T^CATGA
BsrDI        4514  CATTGC               GCAATG(2/0)
BsrGI        1851  TGTACA               T^GTACA
BssHII       1226  GCGCGC               G^CGCGC
BssSI        4415  CTCGTG               CACGAG(-5/-1)
Bst1107I     4670  GTATAC               GTA^TAC
BstZ17I      4670  GTATAC               GTA^TAC
DraI         3566  TTTAAA               TTT^AAA
Eam1105I     5711  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2902  AGCGCT               AGC^GCT
EcoT22I      3493  ATGCAT               ATGCA^T
FseI          441  GGCCGGCC             GGCCGG^CC
FspI         4149  TGCGCA               TGC^GCA
HincII       1097  GTCAAC               GT[CT]^[GA]AC
HindII       1097  GTCAAC               GT[CT]^[GA]AC
KspI         1050  CCGCGG               CCGC^GG
NheI         5490  GCTAGC               G^CTAGC
NruI         3288  TCGCGA               TCG^CGA
NsiI         3493  ATGCAT               ATGCA^T
RcaI         1380  TCATGA               T^CATGA
SacII        1050  CCGCGG               CCGC^GG
ScaI         4899  AGTACT               AGT^ACT
SnaBI        4673  TACGTA               TAC^GTA
SphI          200  GCATGC               GCATG^C
SspBI        1851  TGTACA               T^GTACA
XbaI         3901  TCTAGA               T^CTAGA
XmnI         5622  GAACGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         1829  GTCTAC               GT^[AC][GT]AC
             4670  GTATAC              
AccIII       2953  TCCGGA               T^CCGGA
             5880  TCCGGA              
AspI          331  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4753  GACGGAGTC           
BbrPI        2627  CACGTG               CAC^GTG
             5611  CACGTG              
BsaWI        2953  TCCGGA               [AT]^CCGG[AT]
             5880  TCCGGA              
BseAI        2953  TCCGGA               T^CCGGA
             5880  TCCGGA              
BsiEI        2773  CGGCCG               CG[GA][CT]^CG
             3162  CGGCCG              
BspEI        2953  TCCGGA               T^CCGGA
             5880  TCCGGA              
BsrBI        1028  CCGCTC               CCGCTC(-3/-3)
              415  GAGCGG              
EagI         2773  CGGCCG               C^GGCCG
             3162  CGGCCG              
EclXI        2773  CGGCCG               C^GGCCG
             3162  CGGCCG              
Eco52I       2773  CGGCCG               C^GGCCG
             3162  CGGCCG              
MroI         2953  TCCGGA               T^CCGGA
             5880  TCCGGA              
PflFI         331  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4753  GACGGAGTC           
PmaCI        2627  CACGTG               CAC^GTG
             5611  CACGTG              
PmlI         2627  CACGTG               CAC^GTG
             5611  CACGTG              
SexAI        3389  ACCTGGT              A^CC[AT]GGT
             5115  ACCTGGT             
SspI         4731  AATATT               AAT^ATT
             5303  AATATT              
TfiI         1742  GAATC                G^A[AT]TC
             5868  GATTC               
Tth111I       331  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4753  GACGGAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  135 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PshAI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmaI        
SmlI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI