##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AscI AseI AsnI Asp718I AviII
BcgI BciVI BfrI BsaBI BseAI
BsiWI BspDI BspEI BspHI BssHII
Bst1107I BstZ17I ClaI CpoI EcoRI
EcoRV FspI HpaI KpnI MamI
MfeI MluI MroI MunI NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfiI SnaBI
SphI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 4701 GACGTC GACGT^C
AcsI 4367 AAATTC [GA]^AATT[CT]
AhdI 4739 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3163 GTGCAC G^TGCAC
ApaLI 3163 GTGCAC G^TGCAC
ApoI 4367 AAATTC [GA]^AATT[CT]
Asp700I 2878 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4739 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 1677 TGATCA T^GATCA
BsaWI 1245 ACCGGA [AT]^CCGG[AT]
BsmI 1038 GAATGC GAATGC(1/-1)
Bsp1407I 4401 TGTACA T^GTACA
BsrDI 4405 CATTGC GCAATG(2/0)
BsrGI 4401 TGTACA T^GTACA
BstBI 6672 TTCGAA TT^CGAA
DraI 2480 TTTAAA TTT^AAA
DrdI 5639 GACCGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5523 CGGCCG C^GGCCG
Eam1105I 4739 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5523 CGGCCG C^GGCCG
Eco52I 5523 CGGCCG C^GGCCG
FbaI 1677 TGATCA T^GATCA
NdeI 6612 CATATG CA^TATG
NspV 6672 TTCGAA TT^CGAA
SbfI 6140 CCTGCAGG CCTGCA^GG
SexAI 369 ACCAGGT A^CC[AT]GGT
SfuI 6672 TTCGAA TT^CGAA
SgrAI 123 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 1932 ACTAGT A^CTAGT
Sse8387I 6140 CCTGCAGG CCTGCA^GG
SspBI 4401 TGTACA T^GTACA
XmnI 2878 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 190 GTCTAC GT^[AC][GT]AC
560 GTCTAC
Aor51HI 2752 AGCGCT AGC^GCT
6712 AGCGCT
AspI 368 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4984 GACGGGGTC
BbeI 5089 GGCGCC GGCGC^C
5368 GGCGCC
BsiEI 5523 CGGCCG CG[GA][CT]^CG
5638 CGACCG
BsmBI 6476 GAGACG CGTCTC(1/5)
6548 GAGACG
BsrBI 5526 CCGCTC CCGCTC(-3/-3)
2355 GAGCGG
BstEII 463 GGTGACC G^GT[ACGT]ACC
3675 GGTCACC
BstPI 463 GGTGACC G^GT[ACGT]ACC
3675 GGTCACC
DraIII 5054 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
5620 CACCGCGTG
Eco47III 2752 AGCGCT AGC^GCT
6712 AGCGCT
EcoO65I 463 GGTGACC G^GT[ACGT]ACC
3675 GGTCACC
FseI 4240 GGCCGGCC GGCCGG^CC
6371 GGCCGGCC
HindIII 1834 AAGCTT A^AGCTT
6521 AAGCTT
KasI 5089 GGCGCC G^GCGCC
5368 GGCGCC
NarI 5089 GGCGCC GG^CGCC
5368 GGCGCC
PflFI 368 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4984 GACGGGGTC
PshAI 1667 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2874 GACTGAAGTC
SapI 349 GAAGAGC GCTCTTC(1/4)
175 GCTCTTC
SfoI 5089 GGCGCC GGC^GCC
5368 GGCGCC
TaqI 5120 TCGA T^CGA
6673 TCGA
Tth111I 368 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4984 GACGGGGTC
TthHB8I 5120 TCGA T^CGA
6673 TCGA
XbaI 2903 TCTAGA T^CTAGA
3095 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII AvrII BalI
BamHI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NsiI NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI