##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AccIII AflII AscI
Asp718I AviII AvrII BbrPI BcgI
BclI BfrI BlnI BseAI BsiWI
BspEI Bst1107I BstBI BstZ17I CpoI
EcoRV FbaI FseI FspI HpaI
KpnI MfeI MluI MroI MunI
NotI NruI NspV PacI PmaCI
PmeI PmlI PshAI PvuI RsrII
SalI SapI SbfI SexAI SfuI
SnaBI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AgeI 248 ACCGGT A^CCGGT
AhdI 6326 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3359 CCTCAGG CC^T[ACGT]AGG
AseI 1902 ATTAAT AT^TAAT
AsnI 1902 ATTAAT AT^TAAT
Asp700I 1086 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 6326 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 984 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3537 GCTGAGG CCTCAGC(-5/-2)
BsaWI 248 ACCGGT [AT]^CCGG[AT]
BsmI 2742 GCATTC GAATGC(1/-1)
BspDI 883 ATCGAT AT^CGAT
BspHI 2453 TCATGA T^CATGA
BssHII 29 GCGCGC G^CGCGC
BssSI 5622 CACGAG CACGAG(-5/-1)
Bsu36I 3359 CCTCAGG CC^T[ACGT]AGG
ClaI 883 ATCGAT AT^CGAT
DraI 1481 TTTAAA TTT^AAA
DraIII 3867 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1545 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 6326 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3359 CCTCAGG CC^T[ACGT]AGG
NcoI 1859 CCATGG C^CATGG
NdeI 2550 CATATG CA^TATG
NheI 3794 GCTAGC G^CTAGC
PaeR7I 1837 CTCGAG C^TCGAG
PflFI 984 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 248 ACCGGT A^CCGGT
PshBI 1902 ATTAAT AT^TAAT
RcaI 2453 TCATGA T^CATGA
ScaI 2772 AGTACT AGT^ACT
SgrAI 572 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3713 ACTAGT A^CTAGT
Tth111I 984 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1837 CTCGAG C^TCGAG
XmnI 1086 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 498 GTCTAC GT^[AC][GT]AC
3595 GTAGAC
AclI 2793 AACGTT AA^CGTT
5817 AACGTT
Aor51HI 457 AGCGCT AGC^GCT
4500 AGCGCT
BbsI 1000 GTCTTC GAAGAC(2/6)
3087 GTCTTC
BpuAI 1000 GTCTTC GAAGAC(2/6)
3087 GTCTTC
BsmBI 6420 CGTCTC CGTCTC(1/5)
2852 GAGACG
Bsp1407I 5413 TGTACA T^GTACA
5549 TGTACA
BsrGI 5413 TGTACA T^GTACA
5549 TGTACA
Eco47III 457 AGCGCT AGC^GCT
4500 AGCGCT
EcoNI 3025 CCTAAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3600 CCTGAATCAGG
EcoRI 3549 GAATTC G^AATTC
6506 GAATTC
HincII 1842 GTTGAC GT[CT]^[GA]AC
2215 GTCAAC
HindII 1842 GTTGAC GT[CT]^[GA]AC
2215 GTCAAC
Psp1406I 2793 AACGTT AA^CGTT
5817 AACGTT
SfiI 707 GGCCGGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4012 GGCCTCATTGGCC
SphI 3230 GCATGC GCATG^C
5432 GCATGC
SspBI 5413 TGTACA T^GTACA
5549 TGTACA
SspI 1060 AATATT AAT^ATT
5318 AATATT
StuI 2059 AGGCCT AGG^CCT
2237 AGGCCT
XcmI 1987 CCATCACAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3471 CCAAACAGGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbvI BciVI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoO65I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI