##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccII AclI AflII AgeI
Aor51HI AscI AviII BfrI BsaBI
BsiWI BspDI BspHI BsrFI BssHII
BstBI BstUI Cfr10I ClaI CpoI
DrdI Eco47III EcoRI FseI FspI
HgaI KspI MamI MluI MvnI
NaeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SfiI SfuI SgrAI
SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1784 GGTACC G^GTACC
AccIII 2147 TCCGGA T^CCGGA
AcyI 4392 GGCGCC G[GA]^CG[CT]C
AhdI 1284 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3597 GGGCCC GGGCC^C
AseI 2341 ATTAAT AT^TAAT
AsnI 2341 ATTAAT AT^TAAT
Asp718I 1784 GGTACC G^GTACC
AspEI 1284 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1022 GGATCC G^GATCC
BbeI 4392 GGCGCC GGCGC^C
BbrPI 4686 CACGTG CAC^GTG
BbvCI 5616 GCTGAGG CCTCAGC(-5/-2)
BcgI 1533 GCAGGAGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4548 GCCGAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 4392 GGCGCC G[GA]^CG[CT]C
BsaWI 2147 TCCGGA [AT]^CCGG[AT]
BseAI 2147 TCCGGA T^CCGGA
BsiEI 4546 CGGCCG CG[GA][CT]^CG
Bsp1407I 2471 TGTACA T^GTACA
BspEI 2147 TCCGGA T^CCGGA
BsrBI 3441 CCGCTC CCGCTC(-3/-3)
BsrGI 2471 TGTACA T^GTACA
BssSI 4178 CTCGTG CACGAG(-5/-1)
Bst1107I 1714 GTATAC GTA^TAC
BstZ17I 1714 GTATAC GTA^TAC
EagI 4546 CGGCCG C^GGCCG
Eam1105I 1284 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4546 CGGCCG C^GGCCG
Eco52I 4546 CGGCCG C^GGCCG
EcoRV 3475 GATATC GAT^ATC
HaeII 4392 GGCGCC [GA]GCGC^[CT]
Hin1I 4392 GGCGCC G[GA]^CG[CT]C
HincII 3104 GTTAAC GT[CT]^[GA]AC
HindII 3104 GTTAAC GT[CT]^[GA]AC
HpaI 3104 GTTAAC GTT^AAC
KasI 4392 GGCGCC G^GCGCC
KpnI 1784 GGTACC GGTAC^C
MfeI 3483 CAATTG C^AATTG
MroI 2147 TCCGGA T^CCGGA
MunI 3483 CAATTG C^AATTG
NarI 4392 GGCGCC GG^CGCC
NdeI 2624 CATATG CA^TATG
NheI 2193 GCTAGC G^CTAGC
PmaCI 4686 CACGTG CAC^GTG
PmlI 4686 CACGTG CAC^GTG
PshBI 2341 ATTAAT AT^TAAT
SacI 5674 GAGCTC GAGCT^C
SfoI 4392 GGCGCC GGC^GCC
SnaBI 2346 TACGTA TAC^GTA
SphI 4095 GCATGC GCATG^C
SspBI 2471 TGTACA T^GTACA
XbaI 3752 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Alw44I 611 GTGCAC G^TGCAC
763 GTGCAC
AocI 3612 CCTGAGG CC^T[ACGT]AGG
5313 CCTGAGG
ApaLI 611 GTGCAC G^TGCAC
763 GTGCAC
Asp700I 649 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4995 GAACCACTTC
BalI 1349 TGGCCA TGG^CCA
4455 TGGCCA
BciVI 1116 GTATCC GTATCC(6/5)
2116 GTATCC
BclI 253 TGATCA T^GATCA
5795 TGATCA
BglII 635 AGATCT A^GATCT
1917 AGATCT
BsaAI 2346 TACGTA [CT]AC^GT[GA]
4686 CACGTG
BspLU11I 2537 ACATGT A^CATGT
2555 ACATGT
BstEII 1867 GGTGACC G^GT[ACGT]ACC
3116 GGTCACC
BstPI 1867 GGTGACC G^GT[ACGT]ACC
3116 GGTCACC
Bsu36I 3612 CCTGAGG CC^T[ACGT]AGG
5313 CCTGAGG
DraI 4561 TTTAAA TTT^AAA
4934 TTTAAA
EarI 3228 GAAGAG CTCTTC(1/4)
3630 GAAGAG
Eco81I 3612 CCTGAGG CC^T[ACGT]AGG
5313 CCTGAGG
EcoO65I 1867 GGTGACC G^GT[ACGT]ACC
3116 GGTCACC
EcoT22I 4097 ATGCAT ATGCA^T
4812 ATGCAT
FbaI 253 TGATCA T^GATCA
5795 TGATCA
Ksp632I 3228 GAAGAG CTCTTC(1/4)
3630 GAAGAG
MluNI 1349 TGGCCA TGG^CCA
4455 TGGCCA
MscI 1349 TGGCCA TGG^CCA
4455 TGGCCA
MslI 4608 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5406 CATGCCCATG
NsiI 4097 ATGCAT ATGCA^T
4812 ATGCAT
SbfI 1084 CCTGCAGG CCTGCA^GG
3466 CCTGCAGG
SmaI 4340 CCCGGG CCC^GGG
5767 CCCGGG
SmlI 400 CTTGAG C^T[CT][GA]AG
4247 CTTGAG
Sse8387I 1084 CCTGCAGG CCTGCA^GG
3466 CCTGCAGG
XmaCI 4340 CCCGGG C^CCGGG
5767 CCCGGG
XmaI 4340 CCCGGG C^CCGGG
5767 CCCGGG
XmnI 649 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4995 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII AvrII BanI
BanII BbsI BbvI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SexAI SfaNI
SfcI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII