##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AclI AflII AscI AspI AviII
BclI BfrI BglII BsiWI BsmI
BssHII Bst1107I BstZ17I CpoI DraI
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI PacI PflFI
PmeI Psp1406I RsrII SalI ScaI
SnaBI SpeI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2801 GGTACC G^GTACC
AccI 5481 GTAGAC GT^[AC][GT]AC
AccIII 5530 TCCGGA T^CCGGA
AflIII 2404 ACATGT A^C[GA][CT]GT
AhdI 5968 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 482 AGCGCT AGC^GCT
AseI 6624 ATTAAT AT^TAAT
AsnI 6624 ATTAAT AT^TAAT
Asp718I 2801 GGTACC G^GTACC
AspEI 5968 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3700 CACGTG CAC^GTG
BciVI 3363 GTATCC GTATCC(6/5)
BsaBI 1706 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5530 TCCGGA T^CCGGA
Bsp1407I 3465 TGTACA T^GTACA
BspDI 4695 ATCGAT AT^CGAT
BspEI 5530 TCCGGA T^CCGGA
BspLU11I 2404 ACATGT A^CATGT
BsrGI 3465 TGTACA T^GTACA
BstBI 4673 TTCGAA TT^CGAA
ClaI 4695 ATCGAT AT^CGAT
Eam1105I 5968 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 482 AGCGCT AGC^GCT
HindIII 2867 AAGCTT A^AGCTT
KpnI 2801 GGTACC GGTAC^C
MamI 1706 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 5530 TCCGGA T^CCGGA
NspV 4673 TTCGAA TT^CGAA
PmaCI 3700 CACGTG CAC^GTG
PmlI 3700 CACGTG CAC^GTG
PshBI 6624 ATTAAT AT^TAAT
PvuI 4693 CGATCG CGAT^CG
SexAI 2924 ACCAGGT A^CC[AT]GGT
SfuI 4673 TTCGAA TT^CGAA
SspBI 3465 TGTACA T^GTACA
SspI 2109 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1441 AAATTT [GA]^AATT[CT]
4783 GAATTT
ApoI 1441 AAATTT [GA]^AATT[CT]
4783 GAATTT
Asp700I 4780 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6449 GAACTGCTTC
AvrII 28 CCTAGG C^CTAGG
5852 CCTAGG
BcgI 4549 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
780 GCAGCCGCATCG
BlnI 28 CCTAGG C^CTAGG
5852 CCTAGG
BsaAI 3700 CACGTG [CT]AC^GT[GA]
5478 CACGTA
BsiEI 2057 CGGTCG CG[GA][CT]^CG
4693 CGATCG
BspHI 922 TCATGA T^CATGA
1299 TCATGA
BsrBI 290 CCGCTC CCGCTC(-3/-3)
351 GAGCGG
BstEII 355 GGTTACC G^GT[ACGT]ACC
2332 GGTGACC
BstPI 355 GGTTACC G^GT[ACGT]ACC
2332 GGTGACC
DrdI 1255 GACCAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1277 GACCATGGAGTC
EcoO65I 355 GGTTACC G^GT[ACGT]ACC
2332 GGTGACC
HincII 3961 GTCAAC GT[CT]^[GA]AC
5575 GTTGAC
HindII 3961 GTCAAC GT[CT]^[GA]AC
5575 GTTGAC
PaeR7I 2296 CTCGAG C^TCGAG
2969 CTCGAG
PshAI 2294 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5150 GACGCGGGTC
RcaI 922 TCATGA T^CATGA
1299 TCATGA
SapI 2183 GAAGAGC GCTCTTC(1/4)
3447 GAAGAGC
SfiI 371 GGCCACGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1042 GGCCGTGCTGGCC
SgrAI 240 CACCGGTG C[GA]^CCGG[CT]G
5787 CACCGGTG
XhoI 2296 CTCGAG C^TCGAG
2969 CTCGAG
XmnI 4780 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6449 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AgeI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PinAI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfoI SmaI
SmlI SphI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI