##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvrII       BalI        
BamHI       BbeI        BbsI        BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaI        BsaWI       
BseAI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        DrdI        Eam1105I    
EcoRI       EcoT22I     FbaI        FseI        HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SspBI       SspI        StuI        SwaI        
TfiI        Tsp509I     Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccII         538  CGCG                 CG^CG
Aor51HI        94  AGCGCT               AGC^GCT
AspI          684  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         265  TGCGCA               TGC^GCA
BanI         1380  GGCACC               G^G[CT][GA]CC
BbvCI        1817  GCTGAGG              CCTCAGC(-5/-2)
BsaBI         705  GATGCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         1808  GAATGC               GAATGC(1/-1)
BssSI         372  CTCGTG               CACGAG(-5/-1)
BstUI         538  CGCG                 CG^CG
BstYI         862  AGATCC               [GA]^GATC[CT]
DraIII         45  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1681  CGGCCG               C^GGCCG
EclXI        1681  CGGCCG               C^GGCCG
Eco47III       94  AGCGCT               AGC^GCT
Eco52I       1681  CGGCCG               C^GGCCG
EcoNI          41  CCTGCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1089  GATATC               GAT^ATC
FspI          265  TGCGCA               TGC^GCA
HincII        420  GTCAAC               GT[CT]^[GA]AC
HindII        420  GTCAAC               GT[CT]^[GA]AC
MamI          705  GATGCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          862  AGATCC               [GA]^GATC[CT]
MseI         1857  TTAA                 T^TAA
MvnI          538  CGCG                 CG^CG
PflFI         684  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
ScaI          334  AGTACT               AGT^ACT
SexAI          46  ACCAGGT              A^CC[AT]GGT
SfiI         1469  GGCCTCCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI           55  CCCGGG               CCC^GGG
SphI         1825  GCATGC               GCATG^C
Tru9I        1857  TTAA                 T^TAA
Tth111I       684  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         862  AGATCC               [GA]^GATC[CT]
XmaCI          55  CCCGGG               C^CCGGG
XmaI           55  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI          417  GGCGTC               G[GA]^CG[CT]C
             1686  GGCGTC              
AflIII        424  ACACGT               A^C[GA][CT]GT
              426  ACGTGT              
AocI          530  CCTGAGG              CC^T[ACGT]AGG
             1410  CCTCAGG             
BbrPI         228  CACGTG               CAC^GTG
              425  CACGTG              
BsaAI         228  CACGTG               [CT]AC^GT[GA]
              425  CACGTG              
BsaHI         417  GGCGTC               G[GA]^CG[CT]C
             1686  GGCGTC              
BsrFI         198  GCCGGC               [GA]^CCGG[CT]
             1683  GCCGGC              
BstXI         678  CCAGAGGACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1183  CCATGGACGTGG        
Bsu36I        530  CCTGAGG              CC^T[ACGT]AGG
             1410  CCTCAGG             
Cfr10I        198  GCCGGC               [GA]^CCGG[CT]
             1683  GCCGGC              
Eco81I        530  CCTGAGG              CC^T[ACGT]AGG
             1410  CCTCAGG             
EcoT14I       487  CCATGG               C^C[AT][AT]GG
             1183  CCATGG              
Hin1I         417  GGCGTC               G[GA]^CG[CT]C
             1686  GGCGTC              
NaeI          198  GCCGGC               GCC^GGC
             1683  GCCGGC              
NcoI          487  CCATGG               C^CATGG
             1183  CCATGG              
NgoMIV        198  GCCGGC               G^CCGGC
             1683  GCCGGC              
PmaCI         228  CACGTG               CAC^GTG
              425  CACGTG              
PmlI          228  CACGTG               CAC^GTG
              425  CACGTG              
SacI          276  GAGCTC               GAGCT^C
              725  GAGCTC              
SapI          278  GCTCTTC              GCTCTTC(1/4)
             1505  GCTCTTC             
StyI          487  CCATGG               C^C[AT][AT]GG
             1183  CCATGG              
XcmI          487  CCATGGTCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1270  CCAGCTGGACCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccI        AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoO65I     EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      SbfI        ScrFI       
SfaNI       SfcI        SmlI        Sse8387I    TaqI        
TseI        Tsp45I      TspRI       TthHB8I