##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII Acc65I AclI AhdI Aor51HI
AscI Asp718I AspEI AviII BclI
BsaBI BsiWI BssHII BstBI BstXI
CpoI Eam1105I Eco47III EcoRV EcoT22I
FbaI FseI FspI KpnI KspI
MamI MfeI MunI NdeI NotI
NruI NsiI NspV PacI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 4247 TCCGGA T^CCGGA
AflII 6260 CTTAAG C^TTAAG
AgeI 2926 ACCGGT A^CCGGT
Alw44I 5105 GTGCAC G^TGCAC
ApaI 538 GGGCCC GGGCC^C
ApaLI 5105 GTGCAC G^TGCAC
AspI 1934 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5076 GGATCC G^GATCC
BbeI 152 GGCGCC GGCGC^C
BcgI 100 CGAGCGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3551 GTATCC GTATCC(6/5)
BfrI 6260 CTTAAG C^TTAAG
BglI 131 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 4247 TCCGGA T^CCGGA
BsiEI 54 CGGCCG CG[GA][CT]^CG
BsmI 3019 GAATGC GAATGC(1/-1)
BspDI 2707 ATCGAT AT^CGAT
BspEI 4247 TCCGGA T^CCGGA
BssSI 345 CTCGTG CACGAG(-5/-1)
BstAPI 850 GCATCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2707 ATCGAT AT^CGAT
DrdI 1920 GACAAGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 54 CGGCCG C^GGCCG
EclXI 54 CGGCCG C^GGCCG
Eco52I 54 CGGCCG C^GGCCG
HaeII 152 GGCGCC [GA]GCGC^[CT]
HpaI 4461 GTTAAC GTT^AAC
KasI 152 GGCGCC G^GCGCC
MluI 6391 ACGCGT A^CGCGT
MroI 4247 TCCGGA T^CCGGA
NaeI 52 GCCGGC GCC^GGC
NarI 152 GGCGCC GG^CGCC
NcoI 1496 CCATGG C^CATGG
NgoMIV 52 GCCGGC G^CCGGC
NheI 4749 GCTAGC G^CTAGC
PaeR7I 592 CTCGAG C^TCGAG
PflFI 1934 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1696 CCATATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2926 ACCGGT A^CCGGT
PmeI 4641 GTTTAAAC GTTT^AAAC
ScaI 6264 AGTACT AGT^ACT
SfoI 152 GGCGCC GGC^GCC
SmaI 111 CCCGGG CCC^GGG
Tth111I 1934 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1696 CCATATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5987 TCTAGA T^CTAGA
XhoI 592 CTCGAG C^TCGAG
XmaCI 111 CCCGGG C^CCGGG
XmaI 111 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 46 GGCGTC G[GA]^CG[CT]C
152 GGCGCC
Asp700I 1664 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2999 GAAACATTTC
AvrII 4294 CCTAGG C^CTAGG
5587 CCTAGG
BbrPI 2536 CACGTG CAC^GTG
5052 CACGTG
BbvCI 1801 CCTCAGC CCTCAGC(-5/-2)
4114 CCTCAGC
BglII 3324 AGATCT A^GATCT
4771 AGATCT
BlnI 4294 CCTAGG C^CTAGG
5587 CCTAGG
BsaHI 46 GGCGTC G[GA]^CG[CT]C
152 GGCGCC
BsmBI 6455 CGTCTC CGTCTC(1/5)
3973 GAGACG
BspMI 4923 ACCTGC ACCTGC(4/8)
2370 GCAGGT
BsrBI 101 GAGCGG CCGCTC(-3/-3)
147 GAGCGG
BsrFI 52 GCCGGC [GA]^CCGG[CT]
2926 ACCGGT
BstEII 4741 GGTAACC G^GT[ACGT]ACC
5470 GGTTACC
BstPI 4741 GGTAACC G^GT[ACGT]ACC
5470 GGTTACC
Cfr10I 52 GCCGGC [GA]^CCGG[CT]
2926 ACCGGT
DsaI 1496 CCATGG C^C[GA][CT]GG
3410 CCACGG
EcoNI 2131 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4924 CCTGCCCCAGG
EcoO65I 4741 GGTAACC G^GT[ACGT]ACC
5470 GGTTACC
Hin1I 46 GGCGTC G[GA]^CG[CT]C
152 GGCGCC
HindIII 274 AAGCTT A^AGCTT
5790 AAGCTT
PmaCI 2536 CACGTG CAC^GTG
5052 CACGTG
PmlI 2536 CACGTG CAC^GTG
5052 CACGTG
SacI 14 GAGCTC GAGCT^C
6096 GAGCTC
StuI 2401 AGGCCT AGG^CCT
6360 AGGCCT
XmnI 1664 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2999 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AseI AsnI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BsrDI
BsrGI BsrI BssKI Bst1107I BstF5I
BstNI BstUI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII EaeI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PshBI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII