##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AcyI AgeI Aor51HI
AscI AseI AsnI Asp718I AspI
AviII BamHI BbeI BbrPI BcgI
BclI BlpI Bpu1102I BsaBI BsaHI
BsiWI BspDI BssHII Bst1107I BstBI
BstZ17I CelII ClaI Eco47III FbaI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
PvuI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AclI 1715 AACGTT AA^CGTT
AvrII 2917 CCTAGG C^CTAGG
BanI 4576 GGTGCC G^G[CT][GA]CC
BbvCI 2606 GCTGAGG CCTCAGC(-5/-2)
BglII 4936 AGATCT A^GATCT
BlnI 2917 CCTAGG C^CTAGG
BsiEI 383 CGGCCG CG[GA][CT]^CG
BspHI 3917 TCATGA T^CATGA
CpoI 2942 CGGACCG CG^G[AT]CCG
DraIII 1584 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2745 GACCCTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 383 CGGCCG C^GGCCG
EclXI 383 CGGCCG C^GGCCG
Eco52I 383 CGGCCG C^GGCCG
EcoRV 1404 GATATC GAT^ATC
EcoT22I 3795 ATGCAT ATGCA^T
MfeI 5586 CAATTG C^AATTG
MunI 5586 CAATTG C^AATTG
NsiI 3795 ATGCAT ATGCA^T
PflMI 5555 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3499 GACGACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1715 AACGTT AA^CGTT
PstI 2025 CTGCAG CTGCA^G
RcaI 3917 TCATGA T^CATGA
RsrII 2942 CGGACCG CG^G[AT]CCG
ScaI 4722 AGTACT AGT^ACT
SmaI 739 CCCGGG CCC^GGG
SphI 3942 GCATGC GCATG^C
Van91I 5555 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 739 CCCGGG C^CCGGG
XmaI 739 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3410 GTCTAC GT^[AC][GT]AC
5022 GTCTAC
AccIII 247 TCCGGA T^CCGGA
2940 TCCGGA
AhdI 1660 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2349 GACTACACGTC
Alw44I 526 GTGCAC G^TGCAC
4785 GTGCAC
AocI 2748 CCTAAGG CC^T[ACGT]AGG
4173 CCTAAGG
ApaLI 526 GTGCAC G^TGCAC
4785 GTGCAC
AspEI 1660 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2349 GACTACACGTC
BseAI 247 TCCGGA T^CCGGA
2940 TCCGGA
Bsp1407I 1528 TGTACA T^GTACA
2436 TGTACA
BspEI 247 TCCGGA T^CCGGA
2940 TCCGGA
BspLU11I 1964 ACATGT A^CATGT
2433 ACATGT
BsrBI 389 GAGCGG CCGCTC(-3/-3)
2549 GAGCGG
BsrDI 2779 CATTGC GCAATG(2/0)
3650 GCAATG
BsrFI 227 GCCGGC [GA]^CCGG[CT]
1294 GCCGGC
BsrGI 1528 TGTACA T^GTACA
2436 TGTACA
BssSI 3021 CTCGTG CACGAG(-5/-1)
3906 CTCGTG
BstAPI 350 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5653 GCAGTGTCTGC
BstEII 1922 GGTCACC G^GT[ACGT]ACC
2185 GGTTACC
BstPI 1922 GGTCACC G^GT[ACGT]ACC
2185 GGTTACC
Bsu36I 2748 CCTAAGG CC^T[ACGT]AGG
4173 CCTAAGG
Cfr10I 227 GCCGGC [GA]^CCGG[CT]
1294 GCCGGC
DraI 2512 TTTAAA TTT^AAA
5169 TTTAAA
Eam1105I 1660 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2349 GACTACACGTC
Eco81I 2748 CCTAAGG CC^T[ACGT]AGG
4173 CCTAAGG
EcoO65I 1922 GGTCACC G^GT[ACGT]ACC
2185 GGTTACC
EcoRI 401 GAATTC G^AATTC
3807 GAATTC
HgaI 1985 GACGC GACGC(5/10)
1871 GCGTC
HindIII 5623 AAGCTT A^AGCTT
6048 AAGCTT
MroI 247 TCCGGA T^CCGGA
2940 TCCGGA
NaeI 227 GCCGGC GCC^GGC
1294 GCCGGC
NcoI 295 CCATGG C^CATGG
1419 CCATGG
NgoMIV 227 GCCGGC G^CCGGC
1294 GCCGGC
SapI 4738 GAAGAGC GCTCTTC(1/4)
2108 GCTCTTC
SspBI 1528 TGTACA T^GTACA
2436 TGTACA
XcmI 2038 CCAAGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3144 CCAGGCTGGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AvaI AvaII
BalI BanII BbsI BbvI BciVI
BcnI BfaI BfrI BglI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI