##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AclI AflIII AgeI Aor51HI AscI
AseI AsnI Asp700I BclI BglII
BsaBI BsiWI BspDI BspHI BspLU11I
BsrDI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 5318 GACGTC GACGT^C
Acc65I 2038 GGTACC G^GTACC
AccI 5099 GTCTAC GT^[AC][GT]AC
AccIII 1714 TCCGGA T^CCGGA
AcsI 5081 AAATTT [GA]^AATT[CT]
AflII 3715 CTTAAG C^TTAAG
AhdI 3744 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 5081 AAATTT [GA]^AATT[CT]
Asp718I 2038 GGTACC G^GTACC
AspEI 3744 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3181 GGATCC G^GATCC
BfrI 3715 CTTAAG C^TTAAG
BseAI 1714 TCCGGA T^CCGGA
BsiEI 266 CGGCCG CG[GA][CT]^CG
BsmI 4032 GAATGC GAATGC(1/-1)
Bsp1407I 5476 TGTACA T^GTACA
BspEI 1714 TCCGGA T^CCGGA
BsrFI 4609 GCCGGC [GA]^CCGG[CT]
BsrGI 5476 TGTACA T^GTACA
Cfr10I 4609 GCCGGC [GA]^CCGG[CT]
EagI 266 CGGCCG C^GGCCG
Eam1105I 3744 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 266 CGGCCG C^GGCCG
Eco52I 266 CGGCCG C^GGCCG
HindIII 805 AAGCTT A^AGCTT
KpnI 2038 GGTACC GGTAC^C
MroI 1714 TCCGGA T^CCGGA
NaeI 4609 GCCGGC GCC^GGC
NgoMIV 4609 GCCGGC G^CCGGC
PshAI 3379 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 762 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 5476 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 118 GTGCAC G^TGCAC
5269 GTGCAC
ApaLI 118 GTGCAC G^TGCAC
5269 GTGCAC
BbrPI 2602 CACGTG CAC^GTG
3217 CACGTG
BciVI 2813 GTATCC GTATCC(6/5)
2856 GTATCC
BlpI 2526 GCTCAGC GC^T[ACGT]AGC
4395 GCTGAGC
Bpu1102I 2526 GCTCAGC GC^T[ACGT]AGC
4395 GCTGAGC
BsaAI 2602 CACGTG [CT]AC^GT[GA]
3217 CACGTG
BsaWI 287 TCCGGT [AT]^CCGG[AT]
1714 TCCGGA
BssHII 470 GCGCGC G^CGCGC
731 GCGCGC
BstYI 1610 AGATCC [GA]^GATC[CT]
3181 GGATCC
CelII 2526 GCTCAGC GC^T[ACGT]AGC
4395 GCTGAGC
DraIII 3188 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4841 CACCCCGTG
DrdI 438 GACCGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3070 GACTGAAGGGTC
MflI 1610 AGATCC [GA]^GATC[CT]
3181 GGATCC
NcoI 3013 CCATGG C^CATGG
4522 CCATGG
PflMI 3187 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4840 CCACCCCGTGG
PmaCI 2602 CACGTG CAC^GTG
3217 CACGTG
PmlI 2602 CACGTG CAC^GTG
3217 CACGTG
SphI 1368 GCATGC GCATG^C
2679 GCATGC
Van91I 3187 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4840 CCACCCCGTGG
XcmI 3971 CCATCAGGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4658 CCATCAAAGACATGG
XhoII 1610 AGATCC [GA]^GATC[CT]
3181 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII AviII AvrII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI PvuII RsaI SacI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XmaCI XmaI