##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII Acc65I AflII AgeI AscI
Asp718I AspI BbrPI BcgI BclI
BfrI BsiWI BsmI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
DraIII EcoO65I EcoRI EcoRV FbaI
FseI KpnI MfeI MunI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PvuI SalI SexAI SfiI SfuI
SgrAI SnaBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AocI 4756 CCTCAGG CC^T[ACGT]AGG
Aor51HI 5215 AGCGCT AGC^GCT
ApaI 3666 GGGCCC GGGCC^C
AviII 2317 TGCGCA TGC^GCA
AvrII 4273 CCTAGG C^CTAGG
BciVI 2470 GGATAC GTATCC(6/5)
BlnI 4273 CCTAGG C^CTAGG
BsaAI 2473 TACGTG [CT]AC^GT[GA]
BsaBI 4264 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 3390 TGTACA T^GTACA
BspDI 2337 ATCGAT AT^CGAT
BspHI 2820 TCATGA T^CATGA
BspLU11I 6003 ACATGT A^CATGT
BsrGI 3390 TGTACA T^GTACA
Bsu36I 4756 CCTCAGG CC^T[ACGT]AGG
ClaI 2337 ATCGAT AT^CGAT
DrdI 5548 GACTAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5215 AGCGCT AGC^GCT
Eco81I 4756 CCTCAGG CC^T[ACGT]AGG
EcoT22I 922 ATGCAT ATGCA^T
FspI 2317 TGCGCA TGC^GCA
HpaI 6314 GTTAAC GTT^AAC
KspI 1079 CCGCGG CCGC^GG
MamI 4264 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3373 ACGCGT A^CGCGT
NaeI 142 GCCGGC GCC^GGC
NcoI 1132 CCATGG C^CATGG
NdeI 5428 CATATG CA^TATG
NgoMIV 142 GCCGGC G^CCGGC
NsiI 922 ATGCAT ATGCA^T
PshAI 3370 GACACGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2820 TCATGA T^CATGA
SacII 1079 CCGCGG CCGC^GG
SspBI 3390 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 1968 TCCGGA T^CCGGA
4032 TCCGGA
AclI 3940 AACGTT AA^CGTT
4201 AACGTT
AcsI 4904 AAATTT [GA]^AATT[CT]
6183 GAATTT
AflIII 3373 ACGCGT A^C[GA][CT]GT
6003 ACATGT
AhdI 4 GACGCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4588 GACACAGTGTC
Alw44I 3119 GTGCAC G^TGCAC
3908 GTGCAC
ApaLI 3119 GTGCAC G^TGCAC
3908 GTGCAC
ApoI 4904 AAATTT [GA]^AATT[CT]
6183 GAATTT
AseI 6249 ATTAAT AT^TAAT
6438 ATTAAT
AsnI 6249 ATTAAT AT^TAAT
6438 ATTAAT
AspEI 4 GACGCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4588 GACACAGTGTC
BamHI 599 GGATCC G^GATCC
2106 GGATCC
BbeI 35 GGCGCC GGCGC^C
139 GGCGCC
BglI 3530 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3582 GCCCTGGAGGC
BsaI 299 GAGACC GGTCTC(1/5)
4740 GGTCTC
BseAI 1968 TCCGGA T^CCGGA
4032 TCCGGA
BsiEI 65 CGGCCG CG[GA][CT]^CG
2076 CGGCCG
BspEI 1968 TCCGGA T^CCGGA
4032 TCCGGA
BsrFI 142 GCCGGC [GA]^CCGG[CT]
2716 GCCGGT
Cfr10I 142 GCCGGC [GA]^CCGG[CT]
2716 GCCGGT
CpoI 3023 CGGACCG CG^G[AT]CCG
3435 CGGACCG
DraI 4400 TTTAAA TTT^AAA
5893 TTTAAA
EagI 65 CGGCCG C^GGCCG
2076 CGGCCG
Eam1105I 4 GACGCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4588 GACACAGTGTC
EclXI 65 CGGCCG C^GGCCG
2076 CGGCCG
Eco52I 65 CGGCCG C^GGCCG
2076 CGGCCG
HincII 3703 GTTGAC GT[CT]^[GA]AC
6314 GTTAAC
HindII 3703 GTTGAC GT[CT]^[GA]AC
6314 GTTAAC
KasI 35 GGCGCC G^GCGCC
139 GGCGCC
MroI 1968 TCCGGA T^CCGGA
4032 TCCGGA
NarI 35 GGCGCC GG^CGCC
139 GGCGCC
PshBI 6249 ATTAAT AT^TAAT
6438 ATTAAT
Psp1406I 3940 AACGTT AA^CGTT
4201 AACGTT
RsrII 3023 CGGACCG CG^G[AT]CCG
3435 CGGACCG
SbfI 3283 CCTGCAGG CCTGCA^GG
6030 CCTGCAGG
ScaI 2113 AGTACT AGT^ACT
2853 AGTACT
SfoI 35 GGCGCC GGC^GCC
139 GGCGCC
SmaI 1227 CCCGGG CCC^GGG
1686 CCCGGG
SpeI 4243 ACTAGT A^CTAGT
5791 ACTAGT
SphI 1413 GCATGC GCATG^C
1451 GCATGC
Sse8387I 3283 CCTGCAGG CCTGCA^GG
6030 CCTGCAGG
StuI 1170 AGGCCT AGG^CCT
2658 AGGCCT
XbaI 4475 TCTAGA T^CTAGA
5563 TCTAGA
XmaCI 1227 CCCGGG C^CCGGG
1686 CCCGGG
XmaI 1227 CCCGGG C^CCGGG
1686 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI