##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AscI        
AseI        AsnI        Asp718I     BamHI       BcgI        
BclI        BfrI        BglII       BsiWI       BsmI        
BspDI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HpaI        KpnI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SalI        ScaI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspI        SwaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AcsI         4896  AAATTT               [GA]^AATT[CT]
AflIII       2938  ACATGT               A^C[GA][CT]GT
AgeI          512  ACCGGT               A^CCGGT
AhdI         1602  GACAGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2679  GTGCAC               G^TGCAC
Aor51HI      4656  AGCGCT               AGC^GCT
ApaLI        2679  GTGCAC               G^TGCAC
ApoI         4896  AAATTT               [GA]^AATT[CT]
Asp700I      1401  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1602  GACAGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         657  CCTAGG               C^CTAGG
BciVI        1169  GGATAC               GTATCC(6/5)
BlnI          657  CCTAGG               C^CTAGG
BspHI         896  TCATGA               T^CATGA
BspLU11I     2938  ACATGT               A^CATGT
BstEII       1803  GGTGACC              G^GT[ACGT]ACC
BstPI        1803  GGTGACC              G^GT[ACGT]ACC
Eam1105I     1602  GACAGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     4656  AGCGCT               AGC^GCT
EcoO65I      1803  GGTGACC              G^GT[ACGT]ACC
PaeR7I       1462  CTCGAG               C^TCGAG
PinAI         512  ACCGGT               A^CCGGT
RcaI          896  TCATGA               T^CATGA
SapI         3218  GAAGAGC              GCTCTTC(1/4)
SbfI         3722  CCTGCAGG             CCTGCA^GG
Sse8387I     3722  CCTGCAGG             CCTGCA^GG
XbaI         2919  TCTAGA               T^CTAGA
XhoI         1462  CTCGAG               C^TCGAG
XmnI         1401  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII       1106  TCCGGA               T^CCGGA
             1598  TCCGGA              
AocI         1750  CCTCAGG              CC^T[ACGT]AGG
             2068  CCTCAGG             
BbsI          936  GAAGAC               GAAGAC(2/6)
             1842  GTCTTC              
BlpI         1218  GCTGAGC              GC^T[ACGT]AGC
             3055  GCTCAGC             
Bpu1102I     1218  GCTGAGC              GC^T[ACGT]AGC
             3055  GCTCAGC             
BpuAI         936  GAAGAC               GAAGAC(2/6)
             1842  GTCTTC              
BsaBI         577  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4049  GATCAACATC          
BseAI        1106  TCCGGA               T^CCGGA
             1598  TCCGGA              
BsiEI          56  CGGCCG               CG[GA][CT]^CG
             3077  CGGCCG              
Bsp1407I     1385  TGTACA               T^GTACA
             5178  TGTACA              
BspEI        1106  TCCGGA               T^CCGGA
             1598  TCCGGA              
BsrBI          76  GAGCGG               CCGCTC(-3/-3)
              912  GAGCGG              
BsrDI         434  GCAATG               GCAATG(2/0)
             2431  GCAATG              
BsrGI        1385  TGTACA               T^GTACA
             5178  TGTACA              
BssSI        4359  CACGAG               CACGAG(-5/-1)
             4802  CTCGTG              
Bsu36I       1750  CCTCAGG              CC^T[ACGT]AGG
             2068  CCTCAGG             
CelII        1218  GCTGAGC              GC^T[ACGT]AGC
             3055  GCTCAGC             
DraI         4889  TTTAAA               TTT^AAA
             4976  TTTAAA              
EagI           56  CGGCCG               C^GGCCG
             3077  CGGCCG              
EclXI          56  CGGCCG               C^GGCCG
             3077  CGGCCG              
Eco52I         56  CGGCCG               C^GGCCG
             3077  CGGCCG              
Eco81I       1750  CCTCAGG              CC^T[ACGT]AGG
             2068  CCTCAGG             
HindIII       766  AAGCTT               A^AGCTT
             3914  AAGCTT              
KspI           23  CCGCGG               CCGC^GG
             1808  CCGCGG              
MamI          577  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4049  GATCAACATC          
MroI         1106  TCCGGA               T^CCGGA
             1598  TCCGGA              
SacI          666  GAGCTC               GAGCT^C
             5189  GAGCTC              
SacII          23  CCGCGG               CCGC^GG
             1808  CCGCGG              
SexAI        1681  ACCAGGT              A^CC[AT]GGT
             2978  ACCAGGT             
SspBI        1385  TGTACA               T^GTACA
             5178  TGTACA              
Tsp509I      1820  AATT                 ^AATT
             4897  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AspI        AvaI        AvaII       AviII       BalI        
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DrdI        
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI