##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI BciVI BclI BfrI BglI
BsaBI BsiWI BspDI BsrBI BssHII
Bst1107I BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I FbaI FseI
HgaI HpaI KpnI KspI MamI
MluI NaeI NgoMIV NheI NotI
NruI PacI PflMI PinAI PshAI
PshBI Psp1406I PvuII RsrII SacI
SacII SalI SbfI SfiI SmaI
SnaBI SpeI Sse8387I SwaI Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 64 TCCGGA T^CCGGA
Aor51HI 4119 AGCGCT AGC^GCT
ApaI 3373 GGGCCC GGGCC^C
AviII 125 TGCGCA TGC^GCA
AvrII 2688 CCTAGG C^CTAGG
BamHI 345 GGATCC G^GATCC
BcgI 806 GCAAAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 649 AGATCT A^GATCT
BlnI 2688 CCTAGG C^CTAGG
BlpI 4623 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4623 GCTGAGC GC^T[ACGT]AGC
BseAI 64 TCCGGA T^CCGGA
BsiEI 2136 CGATCG CG[GA][CT]^CG
Bsp1407I 5267 TGTACA T^GTACA
BspEI 64 TCCGGA T^CCGGA
BspHI 1092 TCATGA T^CATGA
BspLU11I 1210 ACATGT A^CATGT
BsrFI 8 GCCGGT [GA]^CCGG[CT]
BsrGI 5267 TGTACA T^GTACA
BssSI 3160 CTCGTG CACGAG(-5/-1)
CelII 4623 GCTGAGC GC^T[ACGT]AGC
Cfr10I 8 GCCGGT [GA]^CCGG[CT]
DraIII 491 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 80 GACACGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4119 AGCGCT AGC^GCT
EcoRV 902 GATATC GAT^ATC
FspI 125 TGCGCA TGC^GCA
HindIII 1565 AAGCTT A^AGCTT
MfeI 40 CAATTG C^AATTG
MroI 64 TCCGGA T^CCGGA
MspA1I 3985 CAGCGG C[AC]G^C[GT]G
MunI 40 CAATTG C^AATTG
NdeI 3991 CATATG CA^TATG
PaeR7I 166 CTCGAG C^TCGAG
PmeI 3700 GTTTAAAC GTTT^AAAC
PvuI 2136 CGATCG CGAT^CG
RcaI 1092 TCATGA T^CATGA
ScaI 1769 AGTACT AGT^ACT
SgrAI 7 CGCCGGTG C[GA]^CCGG[CT]G
SphI 2217 GCATGC GCATG^C
SspBI 5267 TGTACA T^GTACA
XbaI 5303 TCTAGA T^CTAGA
XcmI 4535 CCAGAGGCCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 166 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1634 GTAGAC GT^[AC][GT]AC
1697 GTAGAC
AcyI 920 GGCGCC G[GA]^CG[CT]C
3070 GGCGCC
Alw44I 1258 GTGCAC G^TGCAC
1678 GTGCAC
ApaLI 1258 GTGCAC G^TGCAC
1678 GTGCAC
BanII 3373 GGGCCC G[GA]GC[CT]^C
4837 GGGCTC
BbeI 920 GGCGCC GGCGC^C
3070 GGCGCC
BbrPI 1177 CACGTG CAC^GTG
1675 CACGTG
BsaHI 920 GGCGCC G[GA]^CG[CT]C
3070 GGCGCC
BsaWI 64 TCCGGA [AT]^CCGG[AT]
1192 ACCGGA
BspMI 2873 ACCTGC ACCTGC(4/8)
3737 GCAGGT
BstEII 3825 GGTTACC G^GT[ACGT]ACC
4372 GGTCACC
BstPI 3825 GGTTACC G^GT[ACGT]ACC
4372 GGTCACC
EcoO65I 3825 GGTTACC G^GT[ACGT]ACC
4372 GGTCACC
EcoT22I 619 ATGCAT ATGCA^T
4038 ATGCAT
Hin1I 920 GGCGCC G[GA]^CG[CT]C
3070 GGCGCC
KasI 920 GGCGCC G^GCGCC
3070 GGCGCC
NarI 920 GGCGCC GG^CGCC
3070 GGCGCC
NsiI 619 ATGCAT ATGCA^T
4038 ATGCAT
PmaCI 1177 CACGTG CAC^GTG
1675 CACGTG
PmlI 1177 CACGTG CAC^GTG
1675 CACGTG
SapI 913 GCTCTTC GCTCTTC(1/4)
4091 GCTCTTC
SexAI 4368 ACCAGGT A^CC[AT]GGT
4615 ACCTGGT
SfoI 920 GGCGCC GGC^GCC
3070 GGCGCC
SspI 1867 AATATT AAT^ATT
4998 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BsrDI
BsrI BssKI BstAPI BstBI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI NspV PflFI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII XmnI