##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AflII Alw44I ApaLI AscI
AseI AsnI AviII BbeI BfrI
BglII BsmBI BspDI BspLU11I BssHII
ClaI EagI EclXI Eco52I FseI
FspI KasI MfeI MunI NaeI
NarI NgoMIV NheI NotI NruI
PacI PmeI PshBI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 3609 GGTACC G^GTACC
AclI 6544 AACGTT AA^CGTT
AcyI 1762 GACGCC G[GA]^CG[CT]C
AgeI 3063 ACCGGT A^CCGGT
AhdI 1511 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 6328 CCTGAGG CC^T[ACGT]AGG
Aor51HI 5674 AGCGCT AGC^GCT
Asp700I 4278 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3609 GGTACC G^GTACC
AspEI 1511 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1312 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 784 CCTAGG C^CTAGG
BamHI 4066 GGATCC G^GATCC
BbrPI 2785 CACGTG CAC^GTG
BciVI 3211 GTATCC GTATCC(6/5)
BlnI 784 CCTAGG C^CTAGG
BlpI 4637 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4637 GCTGAGC GC^T[ACGT]AGC
BsaBI 4133 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1762 GACGCC G[GA]^CG[CT]C
BsiWI 5942 CGTACG C^GTACG
BsrDI 5140 GCAATG GCAATG(2/0)
Bst1107I 4120 GTATAC GTA^TAC
BstBI 3321 TTCGAA TT^CGAA
BstEII 3223 GGTGACC G^GT[ACGT]ACC
BstPI 3223 GGTGACC G^GT[ACGT]ACC
BstZ17I 4120 GTATAC GTA^TAC
Bsu36I 6328 CCTGAGG CC^T[ACGT]AGG
CelII 4637 GCTGAGC GC^T[ACGT]AGC
CpoI 2448 CGGACCG CG^G[AT]CCG
DrdI 2102 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1511 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5674 AGCGCT AGC^GCT
Eco81I 6328 CCTGAGG CC^T[ACGT]AGG
EcoNI 2545 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3223 GGTGACC G^GT[ACGT]ACC
Hin1I 1762 GACGCC G[GA]^CG[CT]C
HpaI 6899 GTTAAC GTT^AAC
KpnI 3609 GGTACC GGTAC^C
KspI 2990 CCGCGG CCGC^GG
MamI 4133 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3552 ACGCGT A^CGCGT
NspV 3321 TTCGAA TT^CGAA
PaeR7I 2558 CTCGAG C^TCGAG
PflFI 1312 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3063 ACCGGT A^CCGGT
PmaCI 2785 CACGTG CAC^GTG
PmlI 2785 CACGTG CAC^GTG
PshAI 343 GACGTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6544 AACGTT AA^CGTT
PvuI 2370 CGATCG CGAT^CG
RsrII 2448 CGGACCG CG^G[AT]CCG
SacI 2555 GAGCTC GAGCT^C
SacII 2990 CCGCGG CCGC^GG
ScaI 6456 AGTACT AGT^ACT
SfuI 3321 TTCGAA TT^CGAA
SphI 155 GCATGC GCATG^C
StuI 2442 AGGCCT AGG^CCT
Tth111I 1312 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3196 TCTAGA T^CTAGA
XcmI 3879 CCAATTCCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2558 CTCGAG C^TCGAG
XmnI 4278 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 2228 TCCGGA T^CCGGA
2360 TCCGGA
ApaI 6351 GGGCCC GGGCC^C
6369 GGGCCC
BanI 3576 GGTGCC G^G[CT][GA]CC
3609 GGTACC
BbvCI 2968 CCTCAGC CCTCAGC(-5/-2)
1286 GCTGAGG
BcgI 319 CGAGGTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5321 CGAGTTTTCTGC
BseAI 2228 TCCGGA T^CCGGA
2360 TCCGGA
BsiEI 1826 CGGTCG CG[GA][CT]^CG
2370 CGATCG
BspEI 2228 TCCGGA T^CCGGA
2360 TCCGGA
BspHI 295 TCATGA T^CATGA
1867 TCATGA
BsrBI 267 GAGCGG CCGCTC(-3/-3)
1871 GAGCGG
BssSI 4001 CACGAG CACGAG(-5/-1)
5962 CTCGTG
BstAPI 2781 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3657 GCATGTCCTGC
DraIII 378 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
5896 CACAATGTG
EcoRV 105 GATATC GAT^ATC
629 GATATC
HindIII 3770 AAGCTT A^AGCTT
6156 AAGCTT
MroI 2228 TCCGGA T^CCGGA
2360 TCCGGA
NcoI 2010 CCATGG C^CATGG
4924 CCATGG
PflMI 4000 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4033 CCACAGCCTGG
RcaI 295 TCATGA T^CATGA
1867 TCATGA
SalI 447 GTCGAC G^TCGAC
5908 GTCGAC
Van91I 4000 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4033 CCACAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BclI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI