##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI Aor51HI AscI AseI AsnI
Asp718I BamHI BclI BsaBI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI Eco47III EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SfuI
SgrAI SnaBI SphI SspBI SspI
SwaI TfiI XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 2171 TCCGGA T^CCGGA
AcsI 3965 GAATTT [GA]^AATT[CT]
AflII 1454 CTTAAG C^TTAAG
Alw44I 4651 GTGCAC G^TGCAC
ApaLI 4651 GTGCAC G^TGCAC
ApoI 3965 GAATTT [GA]^AATT[CT]
AspI 856 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1629 TGCGCA TGC^GCA
BbrPI 1820 CACGTG CAC^GTG
BciVI 4842 GGATAC GTATCC(6/5)
BfrI 1454 CTTAAG C^TTAAG
BglII 5142 AGATCT A^GATCT
BsaAI 1820 CACGTG [CT]AC^GT[GA]
BseAI 2171 TCCGGA T^CCGGA
BspEI 2171 TCCGGA T^CCGGA
BspHI 4597 TCATGA T^CATGA
DraI 4049 TTTAAA TTT^AAA
DraIII 876 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3748 GACGGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1629 TGCGCA TGC^GCA
HincII 2126 GTCAAC GT[CT]^[GA]AC
HindII 2126 GTCAAC GT[CT]^[GA]AC
MroI 2171 TCCGGA T^CCGGA
NcoI 990 CCATGG C^CATGG
PaeR7I 184 CTCGAG C^TCGAG
PflFI 856 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1820 CACGTG CAC^GTG
PmlI 1820 CACGTG CAC^GTG
RcaI 4597 TCATGA T^CATGA
SapI 1367 GAAGAGC GCTCTTC(1/4)
ScaI 4188 AGTACT AGT^ACT
SexAI 857 ACCAGGT A^CC[AT]GGT
SfiI 1333 GGCCCCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3437 ACTAGT A^CTAGT
Tth111I 856 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 184 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AhdI 4481 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5623 GACCATCTGTC
AspEI 4481 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5623 GACCATCTGTC
AvrII 3403 CCTAGG C^CTAGG
4646 CCTAGG
BbeI 2542 GGCGCC GGCGC^C
5101 GGCGCC
BbsI 1438 GAAGAC GAAGAC(2/6)
2290 GAAGAC
BlnI 3403 CCTAGG C^CTAGG
4646 CCTAGG
BlpI 5004 GCTCAGC GC^T[ACGT]AGC
5155 GCTCAGC
Bpu1102I 5004 GCTCAGC GC^T[ACGT]AGC
5155 GCTCAGC
BpuAI 1438 GAAGAC GAAGAC(2/6)
2290 GAAGAC
BsaWI 351 ACCGGA [AT]^CCGG[AT]
2171 TCCGGA
BsmI 320 GCATTC GAATGC(1/-1)
4507 GCATTC
BsrDI 811 CATTGC GCAATG(2/0)
3858 CATTGC
BstYI 354 GGATCT [GA]^GATC[CT]
5142 AGATCT
CelII 5004 GCTCAGC GC^T[ACGT]AGC
5155 GCTCAGC
EagI 2518 CGGCCG C^GGCCG
5435 CGGCCG
Eam1105I 4481 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5623 GACCATCTGTC
EclXI 2518 CGGCCG C^GGCCG
5435 CGGCCG
Eco52I 2518 CGGCCG C^GGCCG
5435 CGGCCG
EcoNI 3798 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5110 CCTGCCCGAGG
KasI 2542 GGCGCC G^GCGCC
5101 GGCGCC
MflI 354 GGATCT [GA]^GATC[CT]
5142 AGATCT
NarI 2542 GGCGCC GG^CGCC
5101 GGCGCC
NspI 3474 GCATGT [GA]CATG^[CT]
4954 ACATGC
PshAI 2629 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3071 GACCCCTGTC
SacI 1000 GAGCTC GAGCT^C
5002 GAGCTC
SbfI 1957 CCTGCAGG CCTGCA^GG
4700 CCTGCAGG
SfoI 2542 GGCGCC GGC^GCC
5101 GGCGCC
Sse8387I 1957 CCTGCAGG CCTGCA^GG
4700 CCTGCAGG
TaqI 185 TCGA T^CGA
2053 TCGA
TthHB8I 185 TCGA T^CGA
2053 TCGA
XhoII 354 GGATCT [GA]^GATC[CT]
5142 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XmaCI XmaI XmnI