##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       AclI        AgeI        AhdI        AscI        
AseI        AsnI        AspEI       AvrII       BlnI        
BsaBI       BsiWI       Bsp1407I    BspDI       BspHI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        Eam1105I    EcoT22I     
FseI        HpaI        KspI        MamI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SbfI        
ScaI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Acc65I       2291  GGTACC               G^GTACC
AccI         2197  GTCGAC               GT^[AC][GT]AC
AcsI          321  GAATTC               [GA]^AATT[CT]
AflII        5428  CTTAAG               C^TTAAG
AflIII       1908  ACATGT               A^C[GA][CT]GT
Alw44I       5891  GTGCAC               G^TGCAC
ApaLI        5891  GTGCAC               G^TGCAC
ApoI          321  GAATTC               [GA]^AATT[CT]
Asp718I      2291  GGTACC               G^GTACC
AspI         5098  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        5939  CACGTG               CAC^GTG
BclI         3305  TGATCA               T^GATCA
BfrI         5428  CTTAAG               C^TTAAG
BglII        3518  AGATCT               A^GATCT
BsaAI        5939  CACGTG               [CT]AC^GT[GA]
BsiEI        3152  CGGCCG               CG[GA][CT]^CG
BsmI         3401  GCATTC               GAATGC(1/-1)
BspLU11I     1908  ACATGT               A^CATGT
DraI         1656  TTTAAA               TTT^AAA
EagI         3152  CGGCCG               C^GGCCG
EclXI        3152  CGGCCG               C^GGCCG
Eco52I       3152  CGGCCG               C^GGCCG
EcoRI         321  GAATTC               G^AATTC
EcoRV        3534  GATATC               GAT^ATC
FbaI         3305  TGATCA               T^GATCA
HincII       2197  GTCGAC               GT[CT]^[GA]AC
HindII       2197  GTCGAC               GT[CT]^[GA]AC
KpnI         2291  GGTACC               GGTAC^C
NaeI         5607  GCCGGC               GCC^GGC
NgoMIV       5607  GCCGGC               G^CCGGC
PflFI        5098  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        5939  CACGTG               CAC^GTG
PmlI         5939  CACGTG               CAC^GTG
SalI         2197  GTCGAC               G^TCGAC
SphI         2264  GCATGC               GCATG^C
Tth111I      5098  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII        2239  CGCG                 CG^CG
             4854  CGCG                
AccIII       5012  TCCGGA               T^CCGGA
             5578  TCCGGA              
Aor51HI      2180  AGCGCT               AGC^GCT
             2994  AGCGCT              
Asp700I      4083  GAACTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4899  GAAGTTGTTC          
AviII        4385  TGCGCA               TGC^GCA
             5459  TGCGCA              
BbeI         3222  GGCGCC               GGCGC^C
             3598  GGCGCC              
BcgI         1272  GCAACAACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3894  GCAGCAGGCTCG        
BciVI        1437  GTATCC               GTATCC(6/5)
             5009  GTATCC              
BsaWI        5012  TCCGGA               [AT]^CCGG[AT]
             5578  TCCGGA              
BseAI        5012  TCCGGA               T^CCGGA
             5578  TCCGGA              
BsmBI        5829  CGTCTC               CGTCTC(1/5)
              463  GAGACG              
BspEI        5012  TCCGGA               T^CCGGA
             5578  TCCGGA              
BsrDI        1477  GCAATG               GCAATG(2/0)
             3706  GCAATG              
BstEII       1561  GGTGACC              G^GT[ACGT]ACC
             5238  GGTGACC             
BstPI        1561  GGTGACC              G^GT[ACGT]ACC
             5238  GGTGACC             
BstUI        2239  CGCG                 CG^CG
             4854  CGCG                
DraIII       2526  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3986  CACAGCGTG           
Eco47III     2180  AGCGCT               AGC^GCT
             2994  AGCGCT              
EcoO65I      1561  GGTGACC              G^GT[ACGT]ACC
             5238  GGTGACC             
FspI         4385  TGCGCA               TGC^GCA
             5459  TGCGCA              
KasI         3222  GGCGCC               G^GCGCC
             3598  GGCGCC              
MroI         5012  TCCGGA               T^CCGGA
             5578  TCCGGA              
MvnI         2239  CGCG                 CG^CG
             4854  CGCG                
NarI         3222  GGCGCC               GG^CGCC
             3598  GGCGCC              
SacI         2164  GAGCTC               GAGCT^C
             2827  GAGCTC              
SexAI        1640  ACCAGGT              A^CC[AT]GGT
             2786  ACCTGGT             
SfoI         3222  GGCGCC               GGC^GCC
             3598  GGCGCC              
XmnI         4083  GAACTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4899  GAAGTTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfiI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI