##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbeI        BbrPI       BciVI       BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BspEI       
BsrFI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       CelII       Cfr10I      CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     FseI        FspI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NotI        NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Alw44I       4307  GTGCAC               G^TGCAC
ApaI         1942  GGGCCC               GGGCC^C
ApaLI        4307  GTGCAC               G^TGCAC
BalI         2171  TGGCCA               TGG^CCA
BanI         4871  GGCACC               G^G[CT][GA]CC
BbvCI        1916  CCTCAGC              CCTCAGC(-5/-2)
BclI         5935  TGATCA               T^GATCA
BcnI         1032  CCCGG                CC^[GC]GG
BglII        1657  AGATCT               A^GATCT
BsaAI        5947  CACGTA               [CT]AC^GT[GA]
BspDI        1435  ATCGAT               AT^CGAT
BspMI        5444  GCAGGT               ACCTGC(4/8)
BsrBI         128  GAGCGG               CCGCTC(-3/-3)
Bst1107I      466  GTATAC               GTA^TAC
BstZ17I       466  GTATAC               GTA^TAC
ClaI         1435  ATCGAT               AT^CGAT
DsaI         1341  CCATGG               C^C[GA][CT]GG
EaeI         2171  TGGCCA               [CT]^GGCC[GA]
EcoRV        1124  GATATC               GAT^ATC
EcoT22I      1508  ATGCAT               ATGCA^T
FbaI         5935  TGATCA               T^GATCA
HapII        1033  CCGG                 C^CGG
HpaI         4966  GTTAAC               GTT^AAC
HpaII        1033  CCGG                 C^CGG
MluNI        2171  TGGCCA               TGG^CCA
MscI         2171  TGGCCA               TGG^CCA
MspI         1033  CCGG                 C^CGG
NciI         1032  CCCGG                CC^[GC]GG
NcoI         1341  CCATGG               C^CATGG
NsiI         1508  ATGCAT               ATGCA^T
PaeR7I        394  CTCGAG               C^TCGAG
StuI          799  AGGCCT               AGG^CCT
XbaI         1223  TCTAGA               T^CTAGA
XhoI          394  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII           3  CGCG                 CG^CG
               47  CGCG                
AcyI           21  GGCGTC               G[GA]^CG[CT]C
             3702  GACGCC              
AflII         550  CTTAAG               C^TTAAG
             2194  CTTAAG              
AocI         1929  CCTTAGG              CC^T[ACGT]AGG
             2800  CCTTAGG             
AspI         1234  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3484  GACTAAGTC           
AvaI          247  CCCGAG               C^[CT]CG[GA]G
              394  CTCGAG              
BcgI          313  CGAAGAGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1437  CGATTGAAATGC        
BfrI          550  CTTAAG               C^TTAAG
             2194  CTTAAG              
BsaHI          21  GGCGTC               G[GA]^CG[CT]C
             3702  GACGCC              
BsoBI         247  CCCGAG               C^[CT]CG[GA]G
              394  CTCGAG              
Bsp1407I     2926  TGTACA               T^GTACA
             3594  TGTACA              
BsrGI        2926  TGTACA               T^GTACA
             3594  TGTACA              
BstUI           3  CGCG                 CG^CG
               47  CGCG                
Bsu36I       1929  CCTTAGG              CC^T[ACGT]AGG
             2800  CCTTAGG             
Eco81I       1929  CCTTAGG              CC^T[ACGT]AGG
             2800  CCTTAGG             
EcoRI         618  GAATTC               G^AATTC
             3313  GAATTC              
EcoT14I       935  CCAAGG               C^C[AT][AT]GG
             1341  CCATGG              
HaeII         144  GGCGCT               [GA]GCGC^[CT]
             1063  GGCGCT              
HgaI         3702  GACGC                GACGC(5/10)
               22  GCGTC               
Hin1I          21  GGCGTC               G[GA]^CG[CT]C
             3702  GACGCC              
HindIII       547  AAGCTT               A^AGCTT
              638  AAGCTT              
MaeII        1192  ACGT                 A^CGT
             5948  ACGT                
MvnI            3  CGCG                 CG^CG
               47  CGCG                
PflFI        1234  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3484  GACTAAGTC           
PpuMI        1515  GGGACCC              [GA]G^G[AT]CC[CT]
             3447  AGGTCCC             
PstI          148  CTGCAG               CTGCA^G
             3681  CTGCAG              
SapI          314  GAAGAGC              GCTCTTC(1/4)
             5492  GCTCTTC             
ScaI         1411  AGTACT               AGT^ACT
             5906  AGTACT              
SpeI         4704  ACTAGT               A^CTAGT
             5297  ACTAGT              
SphI          423  GCATGC               GCATG^C
             3073  GCATGC              
SspBI        2926  TGTACA               T^GTACA
             3594  TGTACA              
StyI          935  CCAAGG               C^C[AT][AT]GG
             1341  CCATGG              
SwaI          732  ATTTAAAT             ATTT^AAAT
             5282  ATTTAAAT            
Tth111I      1234  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3484  GACTAAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaII       BanII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspHI       
BspLU11I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        EcoNI       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HincII      HindII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MvaI        MwoI        NdeII       NheI        NlaIII      
NlaIV       NspI        PflMI       PleI        PvuII       
RcaI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
XmnI