##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AviII BbrPI BcgI BclI BfrI
BglII BsaAI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
FbaI FseI FspI HpaI KspI
MfeI MluI MroI MunI NdeI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
ScaI SfuI SgrAI SnaBI SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1247 GGTACC G^GTACC
AflIII 268 ACATGT A^C[GA][CT]GT
Aor51HI 3747 AGCGCT AGC^GCT
Asp718I 1247 GGTACC G^GTACC
AvrII 3766 CCTAGG C^CTAGG
BamHI 2398 GGATCC G^GATCC
BbeI 2773 GGCGCC GGCGC^C
BlnI 3766 CCTAGG C^CTAGG
BlpI 2929 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2929 GCTGAGC GC^T[ACGT]AGC
BsaBI 3989 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2316 GAATGC GAATGC(1/-1)
BspLU11I 268 ACATGT A^CATGT
CelII 2929 GCTGAGC GC^T[ACGT]AGC
DraI 6901 TTTAAA TTT^AAA
Eco47III 3747 AGCGCT AGC^GCT
EcoRV 1223 GATATC GAT^ATC
EcoT22I 5699 ATGCAT ATGCA^T
HindIII 931 AAGCTT A^AGCTT
KasI 2773 GGCGCC G^GCGCC
KpnI 1247 GGTACC GGTAC^C
MamI 3989 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2773 GGCGCC GG^CGCC
NheI 3472 GCTAGC G^CTAGC
NsiI 5699 ATGCAT ATGCA^T
SalI 3516 GTCGAC G^TCGAC
SexAI 2383 ACCTGGT A^CC[AT]GGT
SfoI 2773 GGCGCC GGC^GCC
SpeI 1311 ACTAGT A^CTAGT
XbaI 3827 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 948 GAATTC [GA]^AATT[CT]
2499 GAATTC
ApoI 948 GAATTC [GA]^AATT[CT]
2499 GAATTC
Asp700I 2862 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3163 GAAGCCATTC
AspI 796 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2382 GACCTGGTC
BciVI 4556 GGATAC GTATCC(6/5)
6650 GTATCC
BsaWI 2378 ACCGGA [AT]^CCGG[AT]
4658 ACCGGA
BsmBI 636 CGTCTC CGTCTC(1/5)
5228 CGTCTC
BsrFI 4178 GCCGGC [GA]^CCGG[CT]
5070 GCCGGC
BssSI 1126 CACGAG CACGAG(-5/-1)
1626 CACGAG
BstEII 1215 GGTCACC G^GT[ACGT]ACC
2995 GGTCACC
BstPI 1215 GGTCACC G^GT[ACGT]ACC
2995 GGTCACC
Cfr10I 4178 GCCGGC [GA]^CCGG[CT]
5070 GCCGGC
DrdI 3507 GACCATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6871 GACAGCTAGGTC
EcoO65I 1215 GGTCACC G^GT[ACGT]ACC
2995 GGTCACC
EcoRI 948 GAATTC G^AATTC
2499 GAATTC
HincII 3360 GTCAAC GT[CT]^[GA]AC
3516 GTCGAC
HindII 3360 GTCAAC GT[CT]^[GA]AC
3516 GTCGAC
NaeI 4178 GCCGGC GCC^GGC
5070 GCCGGC
NgoMIV 4178 GCCGGC G^CCGGC
5070 GCCGGC
PflFI 796 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2382 GACCTGGTC
PshAI 1428 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6859 GACCTGTGTC
SbfI 452 CCTGCAGG CCTGCA^GG
1957 CCTGCAGG
SfiI 2080 GGCCCGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4991 GGCCCTGAGGGCC
SphI 1658 GCATGC GCATG^C
5204 GCATGC
Sse8387I 452 CCTGCAGG CCTGCA^GG
1957 CCTGCAGG
TaqI 1373 TCGA T^CGA
3517 TCGA
Tth111I 796 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2382 GACCTGGTC
TthHB8I 1373 TCGA T^CGA
3517 TCGA
XmnI 2862 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3163 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmaCI
XmaI