##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI AocI Aor51HI AscI
Asp700I Asp718I AspEI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsiEI BsiWI BspDI BspHI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SpeI Sse8387I SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 4814 GTCTAC GT^[AC][GT]AC
AccIII 1190 TCCGGA T^CCGGA
AflII 6214 CTTAAG C^TTAAG
AlwNI 572 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2032 GGGCCC GGGCC^C
AspI 3527 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 968 CCCGGG C^[CT]CG[GA]G
BanI 5269 GGTGCC G^G[CT][GA]CC
BciVI 3348 GTATCC GTATCC(6/5)
BclI 2006 TGATCA T^GATCA
BfrI 6214 CTTAAG C^TTAAG
BsaAI 3462 TACGTA [CT]AC^GT[GA]
BsaI 820 GGTCTC GGTCTC(1/5)
BsaWI 1190 TCCGGA [AT]^CCGG[AT]
BseAI 1190 TCCGGA T^CCGGA
BsgI 1599 GTGCAG GTGCAG(16/14)
BsmBI 556 CGTCTC CGTCTC(1/5)
BsoBI 968 CCCGGG C^[CT]CG[GA]G
Bsp1407I 4264 TGTACA T^GTACA
BspEI 1190 TCCGGA T^CCGGA
BspMI 4272 ACCTGC ACCTGC(4/8)
BsrGI 4264 TGTACA T^GTACA
BssSI 2125 CTCGTG CACGAG(-5/-1)
BstEII 2169 GGTGACC G^GT[ACGT]ACC
BstPI 2169 GGTGACC G^GT[ACGT]ACC
EaeI 1343 TGGCCG [CT]^GGCC[GA]
EcoO65I 2169 GGTGACC G^GT[ACGT]ACC
FauI 1939 CCCGC CCCGC(4/6)
FbaI 2006 TGATCA T^GATCA
HaeII 593 AGCGCC [GA]GCGC^[CT]
MroI 1190 TCCGGA T^CCGGA
PacI 2548 TTAATTAA TTAAT^TAA
PflFI 3527 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4662 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 649 GGGTCCC [GA]G^G[AT]CC[CT]
SmaI 968 CCCGGG CCC^GGG
SnaBI 3462 TACGTA TAC^GTA
SspBI 4264 TGTACA T^GTACA
StuI 2232 AGGCCT AGG^CCT
Tth111I 3527 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4662 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3219 TCTAGA T^CTAGA
XmaCI 968 CCCGGG C^CCGGG
XmaI 968 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 5135 ACATGT A^C[GA][CT]GT
5197 ACATGT
AvaII 650 GGTCC G^G[AT]CC
5387 GGTCC
BamHI 2465 GGATCC G^GATCC
3810 GGATCC
BanII 965 GAGCCC G[GA]GC[CT]^C
2032 GGGCCC
BbsI 3283 GAAGAC GAAGAC(2/6)
1304 GTCTTC
BcnI 968 CCCGG CC^[GC]GG
969 CCGGG
BpuAI 3283 GAAGAC GAAGAC(2/6)
1304 GTCTTC
BsmI 5440 GAATGC GAATGC(1/-1)
1569 GCATTC
BspLU11I 5135 ACATGT A^CATGT
5197 ACATGT
DraII 649 GGGTCCC [GA]G^G[ACGT]CC[CT]
2031 GGGGCCC
DsaI 918 CCGTGG C^C[GA][CT]GG
1346 CCGTGG
EcoNI 1554 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2153 CCTGGTGAAGG
EcoO109I 649 GGGTCCC [GA]G^G[ACGT]CC[CT]
2031 GGGGCCC
EcoRI 2139 GAATTC G^AATTC
4008 GAATTC
EcoRV 3236 GATATC GAT^ATC
4408 GATATC
HapII 969 CCGG C^CGG
1191 CCGG
HpaII 969 CCGG C^CGG
1191 CCGG
MspI 969 CCGG C^CGG
1191 CCGG
NciI 968 CCCGG CC^[GC]GG
969 CCGGG
NdeI 1282 CATATG CA^TATG
1993 CATATG
PleI 935 GAGTC GAGTC(4/5)
4613 GAGTC
SmlI 3230 CTCAAG C^T[CT][GA]AG
6214 CTTAAG
SphI 1617 GCATGC GCATG^C
3567 GCATGC
XcmI 2120 CCATTCTCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3836 CCAGCACTGCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaLI ApoI AseI AsnI
AspHI BbvI BfaI BglII BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HinfI HpaI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII