##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII AccIII AgeI AscI AseI
AsnI AviII BseAI BspDI BspEI
Bst1107I BstZ17I ClaI CpoI DraI
EcoRV FseI FspI HpaI KspI
MluI MroI NaeI NdeI NgoMIV
NheI NotI PacI PinAI PmeI
PshBI PvuI RsrII SacII SalI
ScaI SgrAI SnaBI SpeI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 5442 GGTACC G^GTACC
AccI 8303 GTCTAC GT^[AC][GT]AC
AclI 9602 AACGTT AA^CGTT
AflII 7115 CTTAAG C^TTAAG
Asp718I 5442 GGTACC G^GTACC
AspI 9369 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5372 CCTAGG C^CTAGG
BciVI 6375 GGATAC GTATCC(6/5)
BfrI 7115 CTTAAG C^TTAAG
BlnI 5372 CCTAGG C^CTAGG
BsaBI 2029 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 8163 CGTCTC CGTCTC(1/5)
BsrFI 9531 ACCGGC [GA]^CCGG[CT]
BssHII 4877 GCGCGC G^CGCGC
BstEII 1948 GGTGACC G^GT[ACGT]ACC
BstPI 1948 GGTGACC G^GT[ACGT]ACC
Cfr10I 9531 ACCGGC [GA]^CCGG[CT]
EcoO65I 1948 GGTGACC G^GT[ACGT]ACC
KpnI 5442 GGTACC GGTAC^C
MamI 2029 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5515 CAATTG C^AATTG
MunI 5515 CAATTG C^AATTG
NruI 1728 TCGCGA TCG^CGA
PflFI 9369 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 9602 AACGTT AA^CGTT
SbfI 856 CCTGCAGG CCTGCA^GG
SexAI 6452 ACCTGGT A^CC[AT]GGT
SfiI 1192 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 856 CCTGCAGG CCTGCA^GG
Tth111I 9369 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1645 GTGCAC G^TGCAC
6457 GTGCAC
Aor51HI 2790 AGCGCT AGC^GCT
3245 AGCGCT
ApaLI 1645 GTGCAC G^TGCAC
6457 GTGCAC
BbeI 5728 GGCGCC GGCGC^C
7535 GGCGCC
BclI 7284 TGATCA T^GATCA
9454 TGATCA
BsiWI 63 CGTACG C^GTACG
6853 CGTACG
BsmI 9727 GAATGC GAATGC(1/-1)
3756 GCATTC
Bsp1407I 3051 TGTACA T^GTACA
4534 TGTACA
BspLU11I 6521 ACATGT A^CATGT
7503 ACATGT
BsrGI 3051 TGTACA T^GTACA
4534 TGTACA
Eco47III 2790 AGCGCT AGC^GCT
3245 AGCGCT
EcoRI 3187 GAATTC G^AATTC
6040 GAATTC
EcoT22I 8361 ATGCAT ATGCA^T
9729 ATGCAT
FbaI 7284 TGATCA T^GATCA
9454 TGATCA
HincII 4709 GTCAAC GT[CT]^[GA]AC
9366 GTTGAC
HindII 4709 GTCAAC GT[CT]^[GA]AC
9366 GTTGAC
HindIII 7667 AAGCTT A^AGCTT
7983 AAGCTT
KasI 5728 GGCGCC G^GCGCC
7535 GGCGCC
NarI 5728 GGCGCC GG^CGCC
7535 GGCGCC
NsiI 8361 ATGCAT ATGCA^T
9729 ATGCAT
PaeR7I 3614 CTCGAG C^TCGAG
5544 CTCGAG
SfoI 5728 GGCGCC GGC^GCC
7535 GGCGCC
SspBI 3051 TGTACA T^GTACA
4534 TGTACA
StuI 5942 AGGCCT AGG^CCT
6702 AGGCCT
XhoI 3614 CTCGAG C^TCGAG
5544 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspEI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrI BssKI BssSI BstAPI BstBI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI NspV
PflMI PleI PmaCI PmlI PpuMI
PshAI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfuI SmaI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI