##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AcyI AgeI ApaI
AscI AvaI AviII AvrII BbeI
BbrPI BlnI BsaHI BsiEI BsiWI
BsmBI BsoBI BsrBI BssHII BssSI
CpoI EagI EclXI Eco52I FseI
FspI HgaI Hin1I KasI KspI
MluI NarI NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmlI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I StuI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 7639 GGTACC G^GTACC
AccIII 5489 TCCGGA T^CCGGA
AflII 1900 CTTAAG C^TTAAG
AhdI 9567 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 10399 AGCGCT AGC^GCT
Asp718I 7639 GGTACC G^GTACC
AspEI 9567 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6002 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 400 GCTGAGG CCTCAGC(-5/-2)
BcgI 3112 CGAGAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1900 CTTAAG C^TTAAG
BglI 7406 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 8557 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 8557 GCTGAGC GC^T[ACGT]AGC
BseAI 5489 TCCGGA T^CCGGA
BspDI 5795 ATCGAT AT^CGAT
BspEI 5489 TCCGGA T^CCGGA
BsrFI 4346 GCCGGC [GA]^CCGG[CT]
BstBI 4499 TTCGAA TT^CGAA
CelII 8557 GCTGAGC GC^T[ACGT]AGC
Cfr10I 4346 GCCGGC [GA]^CCGG[CT]
ClaI 5795 ATCGAT AT^CGAT
Eam1105I 9567 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 10399 AGCGCT AGC^GCT
EcoNI 7699 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 6250 GCGGG CCCGC(4/6)
HaeII 10399 AGCGCT [GA]GCGC^[CT]
KpnI 7639 GGTACC GGTAC^C
MroI 5489 TCCGGA T^CCGGA
NaeI 4346 GCCGGC GCC^GGC
NgoMIV 4346 GCCGGC G^CCGGC
NspV 4499 TTCGAA TT^CGAA
PflFI 6002 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 10619 GTTTAAAC GTTT^AAAC
PshAI 982 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1260 ACCTGGT A^CC[AT]GGT
SfuI 4499 TTCGAA TT^CGAA
SwaI 251 ATTTAAAT ATTT^AAAT
Tth111I 6002 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 1966 CGCG CG^CG
5765 CGCG
AocI 6212 CCTCAGG CC^T[ACGT]AGG
9051 CCTGAGG
BamHI 3544 GGATCC G^GATCC
4318 GGATCC
BclI 1385 TGATCA T^GATCA
4044 TGATCA
BcnI 1269 CCCGG CC^[GC]GG
6994 CCCGG
BsaAI 1038 CACGTA [CT]AC^GT[GA]
8001 TACGTG
BsaI 7331 GAGACC GGTCTC(1/5)
1462 GGTCTC
BsaWI 5489 TCCGGA [AT]^CCGG[AT]
7117 ACCGGA
BstAPI 5665 GCATGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
8939 GCAACCTTTGC
BstUI 1966 CGCG CG^CG
5765 CGCG
Bsu36I 6212 CCTCAGG CC^T[ACGT]AGG
9051 CCTGAGG
DrdI 2608 GACCCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3654 GACTATTTTGTC
Eco81I 6212 CCTCAGG CC^T[ACGT]AGG
9051 CCTGAGG
EcoRV 5228 GATATC GAT^ATC
5792 GATATC
FbaI 1385 TGATCA T^GATCA
4044 TGATCA
HindIII 7725 AAGCTT A^AGCTT
8959 AAGCTT
HpaI 8392 GTTAAC GTT^AAC
10294 GTTAAC
MvnI 1966 CGCG CG^CG
5765 CGCG
NciI 1269 CCCGG CC^[GC]GG
6994 CCCGG
PstI 3920 CTGCAG CTGCA^G
5137 CTGCAG
SmlI 1900 CTTAAG C^T[CT][GA]AG
2197 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AseI AsnI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BciVI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI Bst1107I BstEII BstF5I BstNI
BstPI BstXI BstYI BstZ17I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NcoI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PshBI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SpeI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI