##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
AseI AsnI Asp718I AspI AviII
AvrII BlnI BseAI BsiWI BspEI
BssHII Bst1107I BstBI BstZ17I CpoI
DrdI EagI EclXI Eco52I EcoNI
FseI FspI KpnI KspI MluI
MroI NaeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I RsrII SacII
SalI ScaI SfiI SfuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AatII 8164 GACGTC GACGT^C
BamHI 6716 GGATCC G^GATCC
BbeI 8306 GGCGCC GGCGC^C
BbrPI 10682 CACGTG CAC^GTG
BglI 2665 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 10016 ACCGGA [AT]^CCGG[AT]
BsiEI 7902 CGATCG CG[GA][CT]^CG
BspDI 7876 ATCGAT AT^CGAT
BstEII 7831 GGTGACC G^GT[ACGT]ACC
BstPI 7831 GGTGACC G^GT[ACGT]ACC
ClaI 7876 ATCGAT AT^CGAT
EcoO65I 7831 GGTGACC G^GT[ACGT]ACC
KasI 8306 GGCGCC G^GCGCC
NarI 8306 GGCGCC GG^CGCC
PmaCI 10682 CACGTG CAC^GTG
PmlI 10682 CACGTG CAC^GTG
PshAI 9123 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 7902 CGATCG CGAT^CG
SbfI 6186 CCTGCAGG CCTGCA^GG
SexAI 8008 ACCTGGT A^CC[AT]GGT
SfoI 8306 GGCGCC GGC^GCC
SgrAI 8932 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 7622 TACGTA TAC^GTA
SpeI 10857 ACTAGT A^CTAGT
SphI 2616 GCATGC GCATG^C
Sse8387I 6186 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 9869 GTAGAC GT^[AC][GT]AC
10318 GTAGAC
AccII 2556 CGCG CG^CG
9491 CGCG
AflII 784 CTTAAG C^TTAAG
7608 CTTAAG
AflIII 4268 ACATGT A^C[GA][CT]GT
10133 ACATGT
AhdI 1356 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3516 GACCCTCAGTC
Alw44I 192 GTGCAC G^TGCAC
3598 GTGCAC
ApaI 6309 GGGCCC GGGCC^C
9210 GGGCCC
ApaLI 192 GTGCAC G^TGCAC
3598 GTGCAC
AspEI 1356 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3516 GACCCTCAGTC
BciVI 4845 GGATAC GTATCC(6/5)
512 GTATCC
BfrI 784 CTTAAG C^TTAAG
7608 CTTAAG
BsaAI 7622 TACGTA [CT]AC^GT[GA]
10682 CACGTG
BspHI 3270 TCATGA T^CATGA
8460 TCATGA
BspLU11I 4268 ACATGT A^CATGT
10133 ACATGT
BstUI 2556 CGCG CG^CG
9491 CGCG
Eam1105I 1356 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3516 GACCCTCAGTC
EcoRI 6919 GAATTC G^AATTC
10919 GAATTC
EcoRV 9646 GATATC GAT^ATC
9724 GATATC
MfeI 2384 CAATTG C^AATTG
6839 CAATTG
MunI 2384 CAATTG C^AATTG
6839 CAATTG
MvnI 2556 CGCG CG^CG
9491 CGCG
NcoI 2429 CCATGG C^CATGG
8273 CCATGG
NheI 1372 GCTAGC G^CTAGC
7477 GCTAGC
RcaI 3270 TCATGA T^CATGA
8460 TCATGA
XbaI 1443 TCTAGA T^CTAGA
5406 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BclI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoO109I EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI