##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Asp718I     AspEI       
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BfrI        BlnI        BsaWI       
BseAI       BsiWI       BsmBI       BsmI        BsoBI       
BspEI       BsrFI       BssSI       Bst1107I    BstZ17I     
Cfr10I      CpoI        Eam1105I    EcoRV       EcoT22I     
FseI        FspI        KasI        KpnI        KspI        
MluI        MroI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SwaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII        1568  CGCG                 CG^CG
AcyI          134  GACGCC               G[GA]^CG[CT]C
Alw44I       7931  GTGCAC               G^TGCAC
Aor51HI      4130  AGCGCT               AGC^GCT
ApaI         5503  GGGCCC               GGGCC^C
ApaLI        7931  GTGCAC               G^TGCAC
AscI         1566  GGCGCGCC             GG^CGCGCC
AspI         3577  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI         436  CCTCAGC              CCTCAGC(-5/-2)
BcnI          840  CCCGG                CC^[GC]GG
BglI            4  GCCATGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         7895  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     7895  GCTAAGC              GC^T[ACGT]AGC
BsaHI         134  GACGCC               G[GA]^CG[CT]C
BsiEI        4379  CGGCCG               CG[GA][CT]^CG
Bsp1407I     6918  TGTACA               T^GTACA
BsrBI        1240  GAGCGG               CCGCTC(-3/-3)
BsrDI        3660  CATTGC               GCAATG(2/0)
BsrGI        6918  TGTACA               T^GTACA
BssHII       1567  GCGCGC               G^CGCGC
BstAPI       3442  GCATTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1568  CGCG                 CG^CG
CelII        7895  GCTAAGC              GC^T[ACGT]AGC
DrdI         1514  GACCAAGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         4379  CGGCCG               C^GGCCG
EclXI        4379  CGGCCG               C^GGCCG
Eco47III     4130  AGCGCT               AGC^GCT
Eco52I       4379  CGGCCG               C^GGCCG
EcoRI        2159  GAATTC               G^AATTC
HapII         841  CCGG                 C^CGG
Hin1I         134  GACGCC               G[GA]^CG[CT]C
HpaI         3895  GTTAAC               GTT^AAC
HpaII         841  CCGG                 C^CGG
MfeI         1538  CAATTG               C^AATTG
MspI          841  CCGG                 C^CGG
MunI         1538  CAATTG               C^AATTG
MvnI         1568  CGCG                 CG^CG
NciI          840  CCCGG                CC^[GC]GG
PflFI        3577  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
ScaI         6588  AGTACT               AGT^ACT
SspBI        6918  TGTACA               T^GTACA
StuI         3608  AGGCCT               AGG^CCT
Tth111I      3577  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

BalI         2554  TGGCCA               TGG^CCA
             6747  TGGCCA              
BciVI        1672  GGATAC               GTATCC(6/5)
             2146  GTATCC              
BglII        1035  AGATCT               A^GATCT
             2603  AGATCT              
BsaAI        3311  TACGTG               [CT]AC^GT[GA]
             7480  TACGTG              
BsaI         1512  GAGACC               GGTCTC(1/5)
             2266  GAGACC              
BspDI         989  ATCGAT               AT^CGAT
             2278  ATCGAT              
BspLU11I     1246  ACATGT               A^CATGT
             7040  ACATGT              
BstEII       4939  GGTCACC              G^GT[ACGT]ACC
             6838  GGTCACC             
BstPI        4939  GGTCACC              G^GT[ACGT]ACC
             6838  GGTCACC             
ClaI          989  ATCGAT               AT^CGAT
             2278  ATCGAT              
DraIII       3857  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4500  CACAGTGTG           
EcoO65I      4939  GGTCACC              G^GT[ACGT]ACC
             6838  GGTCACC             
FauI         5016  CCCGC                CCCGC(4/6)
                9  GCGGG               
HincII       3135  GTCAAC               GT[CT]^[GA]AC
             3895  GTTAAC              
HindII       3135  GTCAAC               GT[CT]^[GA]AC
             3895  GTTAAC              
MluNI        2554  TGGCCA               TGG^CCA
             6747  TGGCCA              
MscI         2554  TGGCCA               TGG^CCA
             6747  TGGCCA              
NcoI         1550  CCATGG               C^CATGG
             3797  CCATGG              
NdeI          199  CATATG               CA^TATG
             3631  CATATG              
PshAI         197  GACATATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4756  GACCTTTGTC          
SapI         2719  GAAGAGC              GCTCTTC(1/4)
             3510  GAAGAGC             
SmlI          964  CTTGAG               C^T[CT][GA]AG
             7762  CTCAAG              
SpeI          262  ACTAGT               A^CTAGT
             4490  ACTAGT              
XcmI         4281  CCAAAGGCTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5525  CCAAGGGTGGAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       AocI        ApoI        AseI        
AsnI        Asp700I     AspHI       AvaII       BanI        
BanII       BbsI        BbvI        BclI        BfaI        
BmyI        BpmI        BpuAI       BsaBI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstBI       BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HgaI        HhaI        
HinP1I      HindIII     HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        NspV        PflMI       
PleI        PpuMI       PshBI       PstI        PvuII       
RcaI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfuI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XbaI        XhoII       XmnI