##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AccI AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII BbrPI BsiEI BsiWI
BspDI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
HpaI KpnI KspI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI PvuI
SacII SalI SbfI ScaI SfiI
SfuI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AclI 6320 AACGTT AA^CGTT
AflII 1356 CTTAAG C^TTAAG
AhdI 8260 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 8260 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4678 CCTAGG C^CTAGG
BalI 7010 TGGCCA TGG^CCA
BfrI 1356 CTTAAG C^TTAAG
BlnI 4678 CCTAGG C^CTAGG
BsaAI 634 TACGTA [CT]AC^GT[GA]
BspHI 6851 TCATGA T^CATGA
BsrDI 3884 CATTGC GCAATG(2/0)
BstAPI 6901 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 64 CGGACCG CG^G[AT]CCG
DraI 8946 TTTAAA TTT^AAA
DraIII 5042 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2228 GACGCAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 8260 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3651 GAATTC G^AATTC
MluNI 7010 TGGCCA TGG^CCA
MscI 7010 TGGCCA TGG^CCA
PflMI 8203 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 6320 AACGTT AA^CGTT
RcaI 6851 TCATGA T^CATGA
RsrII 64 CGGACCG CG^G[AT]CCG
SgrAI 5818 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 634 TACGTA TAC^GTA
SpeI 3273 ACTAGT A^CTAGT
SspI 7252 AATATT AAT^ATT
Van91I 8203 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspHI 109 GAGCTC G[AT]GC[AT]^C
4711 GAGCTC
BbeI 329 GGCGCC GGCGC^C
6281 GGCGCC
BsiHKAI 109 GAGCTC G[AT]GC[AT]^C
4711 GAGCTC
BsmBI 2805 GAGACG CGTCTC(1/5)
5892 GAGACG
BsmI 6602 GAATGC GAATGC(1/-1)
8690 GAATGC
Bsp1407I 203 TGTACA T^GTACA
8955 TGTACA
BsrGI 203 TGTACA T^GTACA
8955 TGTACA
BssSI 379 CACGAG CACGAG(-5/-1)
5501 CTCGTG
BstEII 5669 GGTCACC G^GT[ACGT]ACC
6165 GGTCACC
BstPI 5669 GGTCACC G^GT[ACGT]ACC
6165 GGTCACC
EcoO65I 5669 GGTCACC G^GT[ACGT]ACC
6165 GGTCACC
HincII 4954 GTTGAC GT[CT]^[GA]AC
5586 GTCAAC
HindII 4954 GTTGAC GT[CT]^[GA]AC
5586 GTCAAC
HindIII 2316 AAGCTT A^AGCTT
4661 AAGCTT
KasI 329 GGCGCC G^GCGCC
6281 GGCGCC
MslI 1757 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
7708 CACTCTGATG
NaeI 6655 GCCGGC GCC^GGC
7258 GCCGGC
NarI 329 GGCGCC GG^CGCC
6281 GGCGCC
NgoMIV 6655 GCCGGC G^CCGGC
7258 GCCGGC
SacI 109 GAGCTC GAGCT^C
4711 GAGCTC
SapI 433 GAAGAGC GCTCTTC(1/4)
1505 GCTCTTC
SexAI 1137 ACCAGGT A^CC[AT]GGT
7211 ACCTGGT
SfoI 329 GGCGCC GGC^GCC
6281 GGCGCC
SmaI 187 CCCGGG CCC^GGG
230 CCCGGG
SphI 1497 GCATGC GCATG^C
7676 GCATGC
SspBI 203 TGTACA T^GTACA
8955 TGTACA
XmaCI 187 CCCGGG C^CCGGG
230 CCCGGG
XmaI 187 CCCGGG C^CCGGG
230 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AatII AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspI
AvaI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MroI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PflFI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmnI