##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
Acc65I AclI AscI Asp718I AviII
AvrII BglII BlnI BsaBI BsmI
BssHII Bst1107I BstZ17I CpoI EagI
EclXI Eco52I EcoRI EcoT22I FseI
FspI HpaI KpnI MamI MfeI
MluI MunI NdeI NotI NsiI
PacI PmeI Psp1406I PvuI RsrII
SbfI SgrAI SnaBI SpeI SphI
Sse8387I
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 5356 TCCGGA T^CCGGA
AflII 3300 CTTAAG C^TTAAG
AgeI 2145 ACCGGT A^CCGGT
Aor51HI 8092 AGCGCT AGC^GCT
BfrI 3300 CTTAAG C^TTAAG
BseAI 5356 TCCGGA T^CCGGA
BsiWI 1345 CGTACG C^GTACG
Bsp1407I 3332 TGTACA T^GTACA
BspEI 5356 TCCGGA T^CCGGA
BspLU11I 6848 ACATGT A^CATGT
BsrGI 3332 TGTACA T^GTACA
BssSI 8867 CTCGTG CACGAG(-5/-1)
BstBI 2140 TTCGAA TT^CGAA
BstEII 894 GGTCACC G^GT[ACGT]ACC
BstPI 894 GGTCACC G^GT[ACGT]ACC
Eco47III 8092 AGCGCT AGC^GCT
EcoO65I 894 GGTCACC G^GT[ACGT]ACC
EcoRV 4019 GATATC GAT^ATC
MroI 5356 TCCGGA T^CCGGA
NheI 388 GCTAGC G^CTAGC
NruI 5795 TCGCGA TCG^CGA
NspV 2140 TTCGAA TT^CGAA
PinAI 2145 ACCGGT A^CCGGT
ScaI 3824 AGTACT AGT^ACT
SfiI 3457 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2140 TTCGAA TT^CGAA
SspBI 3332 TGTACA T^GTACA
XbaI 6856 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 5458 GACGTC GACGT^C
6892 GACGTC
AseI 4843 ATTAAT AT^TAAT
7557 ATTAAT
AsnI 4843 ATTAAT AT^TAAT
7557 ATTAAT
Asp700I 1696 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
8547 GAAACTCTTC
BbeI 3408 GGCGCC GGCGC^C
6402 GGCGCC
BbsI 3271 GAAGAC GAAGAC(2/6)
4913 GTCTTC
BpuAI 3271 GAAGAC GAAGAC(2/6)
4913 GTCTTC
BspDI 2005 ATCGAT AT^CGAT
6696 ATCGAT
ClaI 2005 ATCGAT AT^CGAT
6696 ATCGAT
DrdI 327 GACGGTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
886 GACGGTGTGGTC
KasI 3408 GGCGCC G^GCGCC
6402 GGCGCC
KspI 84 CCGCGG CCGC^GG
149 CCGCGG
NarI 3408 GGCGCC GG^CGCC
6402 GGCGCC
PshBI 4843 ATTAAT AT^TAAT
7557 ATTAAT
SacII 84 CCGCGG CCGC^GG
149 CCGCGG
SalI 3625 GTCGAC G^TCGAC
8918 GTCGAC
SfoI 3408 GGCGCC GGC^GCC
6402 GGCGCC
SspI 2112 AATATT AAT^ATT
9014 AATATT
SwaI 6734 ATTTAAAT ATTT^AAAT
8592 ATTTAAAT
XmnI 1696 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
8547 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 169 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AspEI AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PaeR7I PflFI PflMI PleI PmaCI
PmlI PpuMI PshAI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI