##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
AseI AsnI Asp718I AspEI AspI
AviII BalI BamHI BbrPI BclI
BfrI BlpI Bpu1102I BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1097 GACGTC GACGT^C
AcsI 1569 GAATTT [GA]^AATT[CT]
AlwI 2423 GATCC GGATC(4/5)
ApaI 440 GGGCCC GGGCC^C
ApoI 1569 GAATTT [GA]^AATT[CT]
AscI 1683 GGCGCGCC GG^CGCGCC
Asp700I 59 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 283 CCTAGG C^CTAGG
BcgI 930 GCATGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1346 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 132 AGATCT A^GATCT
BlnI 283 CCTAGG C^CTAGG
BsaWI 42 TCCGGT [AT]^CCGG[AT]
BsiEI 607 CGACCG CG[GA][CT]^CG
BsmBI 731 GAGACG CGTCTC(1/5)
BspMI 2230 ACCTGC ACCTGC(4/8)
BstAPI 2357 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 643 GGTCACC G^GT[ACGT]ACC
BstPI 643 GGTCACC G^GT[ACGT]ACC
EcoNI 2022 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 643 GGTCACC G^GT[ACGT]ACC
HindIII 470 AAGCTT A^AGCTT
NaeI 276 GCCGGC GCC^GGC
NcoI 662 CCATGG C^CATGG
NgoMIV 276 GCCGGC G^CCGGC
NspI 231 GCATGC [GA]CATG^[CT]
PaeR7I 1674 CTCGAG C^TCGAG
SphI 231 GCATGC GCATG^C
XbaI 152 TCTAGA T^CTAGA
XcmI 1050 CCATGTTTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1674 CTCGAG C^TCGAG
XmnI 59 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AfaI 436 GTAC GT^AC
677 GTAC
Alw44I 1835 GTGCAC G^TGCAC
2252 GTGCAC
ApaLI 1835 GTGCAC G^TGCAC
2252 GTGCAC
BbeI 581 GGCGCC GGCGC^C
1507 GGCGCC
BciVI 90 GGATAC GTATCC(6/5)
2523 GTATCC
BpmI 1885 CTGGAG CTGGAG(16/14)
1897 CTGGAG
BsaAI 487 TACGTG [CT]AC^GT[GA]
725 TACGTG
BsaI 71 GAGACC GGTCTC(1/5)
667 GAGACC
BseRI 824 GAGGAG GAGGAG(10/8)
1626 GAGGAG
BsmI 350 GCATTC GAATGC(1/-1)
1936 GCATTC
BspHI 750 TCATGA T^CATGA
2351 TCATGA
BsrBI 1265 CCGCTC CCGCTC(-3/-3)
1629 GAGCGG
BsrDI 511 CATTGC GCAATG(2/0)
515 GCAATG
BssHII 1684 GCGCGC G^CGCGC
2436 GCGCGC
BssSI 1325 CTCGTG CACGAG(-5/-1)
2679 CTCGTG
BstXI 957 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1925 CCAGTGCTGTGG
BstYI 132 AGATCT [GA]^GATC[CT]
2422 AGATCC
DsaI 662 CCATGG C^C[GA][CT]GG
864 CCGTGG
EarI 313 GAAGAG CTCTTC(1/4)
2705 GAAGAG
KasI 581 GGCGCC G^GCGCC
1507 GGCGCC
Ksp632I 313 GAAGAG CTCTTC(1/4)
2705 GAAGAG
MflI 132 AGATCT [GA]^GATC[CT]
2422 AGATCC
NarI 581 GGCGCC GG^CGCC
1507 GGCGCC
PpuMI 459 AGGTCCT [GA]G^G[AT]CC[CT]
2242 GGGTCCC
PstI 597 CTGCAG CTGCA^G
2633 CTGCAG
RcaI 750 TCATGA T^CATGA
2351 TCATGA
RsaI 436 GTAC GT^AC
677 GTAC
SacI 493 GAGCTC GAGCT^C
827 GAGCTC
SexAI 2166 ACCTGGT A^CC[AT]GGT
2466 ACCTGGT
SfcI 597 CTGCAG C^T[GA][CT]AG
2633 CTGCAG
SfoI 581 GGCGCC GGC^GCC
1507 GGCGCC
SmaI 1347 CCCGGG CCC^GGG
2239 CCCGGG
StuI 720 AGGCCT AGG^CCT
1353 AGGCCT
XhoII 132 AGATCT [GA]^GATC[CT]
2422 AGATCC
XmaCI 1347 CCCGGG C^CCGGG
2239 CCCGGG
XmaI 1347 CCCGGG C^CCGGG
2239 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpuAI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I