##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BbvCI BclI
BlpI Bpu1102I BsaBI BsiWI BsmBI
Bsp1407I BspHI BsrGI BssHII Bst1107I
BstZ17I CelII CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoRV FbaI
FseI FspI HpaI KpnI KspI
MamI MfeI MunI NdeI NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1995 GACGTC GACGT^C
AccI 842 GTCTAC GT^[AC][GT]AC
AccIII 2937 TCCGGA T^CCGGA
AflII 3309 CTTAAG C^TTAAG
AspI 836 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3328 CCTAGG C^CTAGG
BamHI 1681 GGATCC G^GATCC
BbrPI 1062 CACGTG CAC^GTG
BcgI 2986 CGATCATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2587 GTATCC GTATCC(6/5)
BfrI 3309 CTTAAG C^TTAAG
BglII 4096 AGATCT A^GATCT
BlnI 3328 CCTAGG C^CTAGG
BsaAI 1062 CACGTG [CT]AC^GT[GA]
BsaWI 2937 TCCGGA [AT]^CCGG[AT]
BseAI 2937 TCCGGA T^CCGGA
BspDI 2568 ATCGAT AT^CGAT
BspEI 2937 TCCGGA T^CCGGA
BstAPI 2286 GCAATAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2568 ATCGAT AT^CGAT
EagI 296 CGGCCG C^GGCCG
EclXI 296 CGGCCG C^GGCCG
Eco52I 296 CGGCCG C^GGCCG
EcoT22I 4197 ATGCAT ATGCA^T
MluI 1788 ACGCGT A^CGCGT
MroI 2937 TCCGGA T^CCGGA
MslI 4279 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 4197 ATGCAT ATGCA^T
PflFI 836 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1062 CACGTG CAC^GTG
PmlI 1062 CACGTG CAC^GTG
PvuII 3245 CAGCTG CAG^CTG
ScaI 1386 AGTACT AGT^ACT
SfiI 3118 GGCCTGATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 744 CGCCGGCG C[GA]^CCGG[CT]G
SspI 4104 AATATT AAT^ATT
Tth111I 836 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 3019 CCTTAGG CC^T[ACGT]AGG
3628 CCTTAGG
Asp700I 590 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1907 GAAGGCCTTC
BbeI 97 GGCGCC GGCGC^C
911 GGCGCC
BsmI 445 GAATGC GAATGC(1/-1)
3766 GCATTC
BspLU11I 2692 ACATGT A^CATGT
4263 ACATGT
BsrFI 624 GCCGGC [GA]^CCGG[CT]
745 GCCGGC
BssSI 2327 CACGAG CACGAG(-5/-1)
2049 CTCGTG
BstBI 1326 TTCGAA TT^CGAA
1989 TTCGAA
BstXI 1798 CCAGACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2814 CCACACATCTGG
Bsu36I 3019 CCTTAGG CC^T[ACGT]AGG
3628 CCTTAGG
Cfr10I 624 GCCGGC [GA]^CCGG[CT]
745 GCCGGC
Eco81I 3019 CCTTAGG CC^T[ACGT]AGG
3628 CCTTAGG
HincII 1231 GTCAAC GT[CT]^[GA]AC
3861 GTCAAC
HindII 1231 GTCAAC GT[CT]^[GA]AC
3861 GTCAAC
HindIII 3306 AAGCTT A^AGCTT
4320 AAGCTT
KasI 97 GGCGCC G^GCGCC
911 GGCGCC
NaeI 624 GCCGGC GCC^GGC
745 GCCGGC
NarI 97 GGCGCC GG^CGCC
911 GGCGCC
NcoI 3160 CCATGG C^CATGG
3967 CCATGG
NgoMIV 624 GCCGGC G^CCGGC
745 GCCGGC
NspV 1326 TTCGAA TT^CGAA
1989 TTCGAA
SfoI 97 GGCGCC GGC^GCC
911 GGCGCC
SfuI 1326 TTCGAA TT^CGAA
1989 TTCGAA
SmaI 599 CCCGGG CCC^GGG
905 CCCGGG
SphI 1000 GCATGC GCATG^C
4199 GCATGC
XcmI 2360 CCATAAATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2690 CCACATGTCAAATGG
XmaCI 599 CCCGGG C^CCGGG
905 CCCGGG
XmaI 599 CCCGGG C^CCGGG
905 CCCGGG
XmnI 590 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1907 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII