##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BbeI        
BciVI       BclI        BlnI        BsaWI       BseAI       
BsiEI       BsmBI       BspDI       BspEI       BssHII      
Bst1107I    BstZ17I     ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      FbaI        
FspI        KasI        KpnI        KspI        MluI        
MroI        NarI        NotI        NruI        PacI        
PaeR7I      PflMI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SfoI        SgrAI       SnaBI       SpeI        
Sse8387I    SwaI        Van91I      XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII         410  GACGTC               GACGT^C
AccI         5056  GTAGAC               GT^[AC][GT]AC
AccII        2492  CGCG                 CG^CG
Alw44I       2471  GTGCAC               G^TGCAC
ApaLI        2471  GTGCAC               G^TGCAC
BamHI         833  GGATCC               G^GATCC
BbrPI        5738  CACGTG               CAC^GTG
BlpI          937  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      937  GCTGAGC              GC^T[ACGT]AGC
BsaAI        5738  CACGTG               [CT]AC^GT[GA]
BsaBI         801  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI        6648  CGTACG               C^GTACG
BspHI        4823  TCATGA               T^CATGA
BsrFI        6499  GCCGGC               [GA]^CCGG[CT]
BstBI         974  TTCGAA               TT^CGAA
BstEII        214  GGTCACC              G^GT[ACGT]ACC
BstPI         214  GGTCACC              G^GT[ACGT]ACC
BstUI        2492  CGCG                 CG^CG
CelII         937  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       6499  GCCGGC               [GA]^CCGG[CT]
DraIII       5781  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4355  GACCCTGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       214  GGTCACC              G^GT[ACGT]ACC
EcoRV        5898  GATATC               GAT^ATC
EcoT22I      1213  ATGCAT               ATGCA^T
FseI         6498  GGCCGGCC             GGCCGG^CC
HaeII        5762  AGCGCC               [GA]GCGC^[CT]
HpaI         4158  GTTAAC               GTT^AAC
MamI          801  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         2492  CGCG                 CG^CG
NaeI         6499  GCCGGC               GCC^GGC
NgoMIV       6499  GCCGGC               G^CCGGC
NsiI         1213  ATGCAT               ATGCA^T
NspV          974  TTCGAA               TT^CGAA
PmaCI        5738  CACGTG               CAC^GTG
PmlI         5738  CACGTG               CAC^GTG
PshAI        3411  GACCATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         4823  TCATGA               T^CATGA
SacI         4469  GAGCTC               GAGCT^C
ScaI         1366  AGTACT               AGT^ACT
SfiI         6580  GGCCAGCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          974  TTCGAA               TT^CGAA
SmaI         3319  CCCGGG               CCC^GGG
SphI         2079  GCATGC               GCATG^C
SspI         4644  AATATT               AAT^ATT
StuI         4058  AGGCCT               AGG^CCT
XmaCI        3319  CCCGGG               C^CCGGG
XmaI         3319  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          410  GACGTC               G[GA]^CG[CT]C
             2866  GACGCC              
AocI         2099  CCTCAGG              CC^T[ACGT]AGG
             3486  CCTGAGG             
AspI         4303  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5302  GACATGGTC           
AvaI         1034  CTCGGG               C^[CT]CG[GA]G
             3319  CCCGGG              
BanI         3534  GGTGCC               G^G[CT][GA]CC
             3552  GGCACC              
BbvCI        4551  CCTCAGC              CCTCAGC(-5/-2)
             6543  GCTGAGG             
BcgI         4955  GCATGTACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             6678  GCAGGAGAATCG        
BglII        1995  AGATCT               A^GATCT
             6256  AGATCT              
BsaHI         410  GACGTC               G[GA]^CG[CT]C
             2866  GACGCC              
BsmI         1230  GAATGC               GAATGC(1/-1)
             5812  GCATTC              
BsoBI        1034  CTCGGG               C^[CT]CG[GA]G
             3319  CCCGGG              
BsrDI        4564  CATTGC               GCAATG(2/0)
             5518  CATTGC              
BssSI        4558  CTCGTG               CACGAG(-5/-1)
             4963  CTCGTG              
BstAPI       5760  GCAGCGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             6704  GCAGAGGCTGC         
Bsu36I       2099  CCTCAGG              CC^T[ACGT]AGG
             3486  CCTGAGG             
CfoI         2491  GCGC                 GCG^C
             5763  GCGC                
DraI          306  TTTAAA               TTT^AAA
             5225  TTTAAA              
Eco81I       2099  CCTCAGG              CC^T[ACGT]AGG
             3486  CCTGAGG             
EcoRI        4819  GAATTC               G^AATTC
             4969  GAATTC              
HgaI         2866  GACGC                GACGC(5/10)
             2976  GACGC               
HhaI         2491  GCGC                 GCG^C
             5763  GCGC                
Hin1I         410  GACGTC               G[GA]^CG[CT]C
             2866  GACGCC              
HinP1I       2491  GCGC                 G^CGC
             5763  GCGC                
HindIII       734  AAGCTT               A^AGCTT
             1054  AAGCTT              
NcoI         2690  CCATGG               C^CATGG
             3781  CCATGG              
NdeI         4348  CATATG               CA^TATG
             6141  CATATG              
PflFI        4303  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5302  GACATGGTC           
PpuMI           5  GGGACCT              [GA]G^G[AT]CC[CT]
              842  GGGACCT             
SapI         2569  GAAGAGC              GCTCTTC(1/4)
             2839  GCTCTTC             
SexAI        2272  ACCAGGT              A^CC[AT]GGT
             3724  ACCTGGT             
Tth111I      4303  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5302  GACATGGTC           
XbaI         4799  TCTAGA               T^CTAGA
             6049  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaII       BalI        BanII       BbsI        
BbvI        BcnI        BfaI        BfrI        BglI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    Bsp1407I    BspLU11I    
BspMI       BsrBI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MfeI        MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MunI        MvaI        MwoI        
NciI        NdeII       NheI        NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI