##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AgeI AhdI AocI
Aor51HI ApaI AscI AspEI BalI
BamHI BbvCI BcgI BsaBI BsiWI
BspDI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV FseI MamI
MluI MluNI MscI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PpuMI PshAI Psp1406I PvuI RsrII
SalI SapI SfiI SfuI SnaBI
SphI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 2608 GTCTAC GT^[AC][GT]AC
AccIII 636 TCCGGA T^CCGGA
AflII 2583 CTTAAG C^TTAAG
Asp700I 4387 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4887 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 666 TGCGCA TGC^GCA
BbrPI 3589 CACGTG CAC^GTG
BciVI 5195 GGATAC GTATCC(6/5)
BclI 5961 TGATCA T^GATCA
BfrI 2583 CTTAAG C^TTAAG
BsaWI 636 TCCGGA [AT]^CCGG[AT]
BseAI 636 TCCGGA T^CCGGA
BsiEI 820 CGACCG CG[GA][CT]^CG
BspEI 636 TCCGGA T^CCGGA
BspLU11I 5820 ACATGT A^CATGT
BsrBI 3662 GAGCGG CCGCTC(-3/-3)
BstAPI 239 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1574 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1105 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1640 ATGCAT ATGCA^T
FbaI 5961 TGATCA T^GATCA
FspI 666 TGCGCA TGC^GCA
HpaI 3619 GTTAAC GTT^AAC
MfeI 5112 CAATTG C^AATTG
MroI 636 TCCGGA T^CCGGA
MunI 5112 CAATTG C^AATTG
NaeI 17 GCCGGC GCC^GGC
NcoI 4039 CCATGG C^CATGG
NgoMIV 17 GCCGGC G^CCGGC
NheI 6315 GCTAGC G^CTAGC
NsiI 1640 ATGCAT ATGCA^T
PflFI 4887 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2920 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3589 CACGTG CAC^GTG
PmlI 3589 CACGTG CAC^GTG
SacI 1783 GAGCTC GAGCT^C
SgrAI 16 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 6339 ACTAGT A^CTAGT
Tth111I 4887 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2920 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 4387 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 14 GGCGCC G[GA]^CG[CT]C
35 GGCGCC
AlwI 457 GATCC GGATC(4/5)
3580 GGATC
AvrII 4450 CCTAGG C^CTAGG
5394 CCTAGG
BbeI 14 GGCGCC GGCGC^C
35 GGCGCC
BglI 2820 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3382 GCCTCAGAGGC
BglII 582 AGATCT A^GATCT
1389 AGATCT
BlnI 4450 CCTAGG C^CTAGG
5394 CCTAGG
BlpI 1030 GCTGAGC GC^T[ACGT]AGC
6319 GCTAAGC
Bpu1102I 1030 GCTGAGC GC^T[ACGT]AGC
6319 GCTAAGC
BsaAI 1505 TACGTG [CT]AC^GT[GA]
3589 CACGTG
BsaHI 14 GGCGCC G[GA]^CG[CT]C
35 GGCGCC
BsmI 3898 GAATGC GAATGC(1/-1)
4034 GCATTC
BspHI 2560 TCATGA T^CATGA
4305 TCATGA
BsrDI 4220 CATTGC GCAATG(2/0)
6529 CATTGC
BsrFI 17 GCCGGC [GA]^CCGG[CT]
822 ACCGGC
BstEII 2000 GGTAACC G^GT[ACGT]ACC
6269 GGTAACC
BstPI 2000 GGTAACC G^GT[ACGT]ACC
6269 GGTAACC
CelII 1030 GCTGAGC GC^T[ACGT]AGC
6319 GCTAAGC
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
822 ACCGGC
DraII 1101 AGGGCCT [GA]G^G[ACGT]CC[CT]
4010 GGGGCCT
EcoO109I 1101 AGGGCCT [GA]G^G[ACGT]CC[CT]
4010 GGGGCCT
EcoO65I 2000 GGTAACC G^GT[ACGT]ACC
6269 GGTAACC
Hin1I 14 GGCGCC G[GA]^CG[CT]C
35 GGCGCC
HindIII 660 AAGCTT A^AGCTT
987 AAGCTT
KasI 14 GGCGCC G^GCGCC
35 GGCGCC
KspI 39 CCGCGG CCGC^GG
700 CCGCGG
NarI 14 GGCGCC GG^CGCC
35 GGCGCC
NdeI 1277 CATATG CA^TATG
6298 CATATG
RcaI 2560 TCATGA T^CATGA
4305 TCATGA
SacII 39 CCGCGG CCGC^GG
700 CCGCGG
SbfI 175 CCTGCAGG CCTGCA^GG
1096 CCTGCAGG
SexAI 2531 ACCAGGT A^CC[AT]GGT
6121 ACCTGGT
SfoI 14 GGCGCC GGC^GCC
35 GGCGCC
SmaI 1145 CCCGGG CCC^GGG
1164 CCCGGG
Sse8387I 175 CCTGCAGG CCTGCA^GG
1096 CCTGCAGG
SspI 4863 AATATT AAT^ATT
6443 AATATT
XbaI 4900 TCTAGA T^CTAGA
5645 TCTAGA
XcmI 2456 CCAAAGGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5800 CCAAATTTGTAATGG
XmaCI 1145 CCCGGG C^CCGGG
1164 CCCGGG
XmaI 1145 CCCGGG C^CCGGG
1164 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwNI ApaLI
ApoI AseI AsnI Asp718I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII