##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BcnI BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspEI BsrBI BssHII BstBI
BstUI CpoI DsaI EagI Eam1105I
EclXI Eco52I EcoRV FseI FspI
Hin1I HpaI KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NarI NciI NcoI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2667 GTATAC GT^[AC][GT]AC
Aor51HI 2085 AGCGCT AGC^GCT
BamHI 4481 GGATCC G^GATCC
BanI 2276 GGTGCC G^G[CT][GA]CC
BclI 3748 TGATCA T^GATCA
BsaAI 1966 CACGTA [CT]AC^GT[GA]
Bsp1407I 2700 TGTACA T^GTACA
BspDI 462 ATCGAT AT^CGAT
BspLU11I 6145 ACATGT A^CATGT
BsrFI 4222 GCCGGC [GA]^CCGG[CT]
BsrGI 2700 TGTACA T^GTACA
BssSI 2132 CACGAG CACGAG(-5/-1)
Bst1107I 2667 GTATAC GTA^TAC
BstXI 1475 CCATGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2667 GTATAC GTA^TAC
CfoI 2086 GCGC GCG^C
Cfr10I 4222 GCCGGC [GA]^CCGG[CT]
ClaI 462 ATCGAT AT^CGAT
DraIII 3479 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4376 GACATCCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4093 TGGCCG [CT]^GGCC[GA]
Eco47III 2085 AGCGCT AGC^GCT
FbaI 3748 TGATCA T^GATCA
HaeII 2085 AGCGCT [GA]GCGC^[CT]
HhaI 2086 GCGC GCG^C
HinP1I 2086 GCGC G^CGC
MfeI 2465 CAATTG C^AATTG
MunI 2465 CAATTG C^AATTG
NaeI 4222 GCCGGC GCC^GGC
NgoMIV 4222 GCCGGC G^CCGGC
PflMI 4568 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2470 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 2714 AGTACT AGT^ACT
SpeI 6116 ACTAGT A^CTAGT
SphI 1050 GCATGC GCATG^C
SspBI 2700 TGTACA T^GTACA
StuI 6331 AGGCCT AGG^CCT
SwaI 3344 ATTTAAAT ATTT^AAAT
Van91I 4568 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 816 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 1209 ACGTGT A^C[GA][CT]GT
6145 ACATGT
AocI 5443 CCTCAGG CC^T[ACGT]AGG
6334 CCTCAGG
Asp700I 1036 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1924 GAAAAACTTC
BciVI 2323 GGATAC GTATCC(6/5)
2416 GGATAC
BglI 4048 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6333 GCCTCAGGGGC
BspHI 1062 TCATGA T^CATGA
3047 TCATGA
BsrDI 582 CATTGC GCAATG(2/0)
376 GCAATG
Bsu36I 5443 CCTCAGG CC^T[ACGT]AGG
6334 CCTCAGG
Eco81I 5443 CCTCAGG CC^T[ACGT]AGG
6334 CCTCAGG
EcoRI 5048 GAATTC G^AATTC
5805 GAATTC
EcoT14I 609 CCAAGG C^C[AT][AT]GG
792 CCTTGG
EcoT22I 800 ATGCAT ATGCA^T
2912 ATGCAT
NheI 204 GCTAGC G^CTAGC
5164 GCTAGC
NsiI 800 ATGCAT ATGCA^T
2912 ATGCAT
PaeR7I 1181 CTCGAG C^TCGAG
1615 CTCGAG
PstI 3397 CTGCAG CTGCA^G
4027 CTGCAG
RcaI 1062 TCATGA T^CATGA
3047 TCATGA
SexAI 2480 ACCAGGT A^CC[AT]GGT
4573 ACCTGGT
StyI 609 CCAAGG C^C[AT][AT]GG
792 CCTTGG
XhoI 1181 CTCGAG C^TCGAG
1615 CTCGAG
XmnI 1036 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1924 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BanII BbsI BbvI
BfaI BfrI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII EarI EcoNI EcoO109I
EcoO65I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII