##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AcyI AgeI Alw44I
Aor51HI ApaI ApaLI AscI AspI
AviII BamHI BbeI BbrPI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I FseI
FspI HaeII HgaI Hin1I HpaI
KasI KspI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmlI PshAI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2457 GGTACC G^GTACC
AccI 6800 GTCTAC GT^[AC][GT]AC
AccII 2170 CGCG CG^CG
AclI 1401 AACGTT AA^CGTT
AhdI 3361 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4376 CCTCAGG CC^T[ACGT]AGG
AseI 6242 ATTAAT AT^TAAT
AsnI 6242 ATTAAT AT^TAAT
Asp718I 2457 GGTACC G^GTACC
AspEI 3361 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3448 CCTAGG C^CTAGG
BcgI 1815 GCAGATGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4404 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5559 AGATCT A^GATCT
BlnI 3448 CCTAGG C^CTAGG
BsaAI 908 CACGTA [CT]AC^GT[GA]
BsaBI 5497 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 4260 GCAGGT ACCTGC(4/8)
BstUI 2170 CGCG CG^CG
Bsu36I 4376 CCTCAGG CC^T[ACGT]AGG
CfoI 2171 GCGC GCG^C
Eam1105I 3361 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4376 CCTCAGG CC^T[ACGT]AGG
FauI 426 GCGGG CCCGC(4/6)
HapII 53 CCGG C^CGG
HhaI 2171 GCGC GCG^C
HinP1I 2171 GCGC G^CGC
HpaII 53 CCGG C^CGG
KpnI 2457 GGTACC GGTAC^C
MamI 5497 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 53 CCGG C^CGG
MvnI 2170 CGCG CG^CG
NdeI 3835 CATATG CA^TATG
PmeI 6322 GTTTAAAC GTTT^AAAC
PshBI 6242 ATTAAT AT^TAAT
Psp1406I 1401 AACGTT AA^CGTT
SexAI 5691 ACCAGGT A^CC[AT]GGT
SmaI 52 CCCGGG CCC^GGG
StuI 2584 AGGCCT AGG^CCT
XbaI 7898 TCTAGA T^CTAGA
XmaCI 52 CCCGGG C^CCGGG
XmaI 52 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 3780 CTTAAG C^TTAAG
6790 CTTAAG
BalI 5799 TGGCCA TGG^CCA
7261 TGGCCA
BbvCI 2461 CCTCAGC CCTCAGC(-5/-2)
4882 CCTCAGC
BclI 5457 TGATCA T^GATCA
6832 TGATCA
BcnI 52 CCCGG CC^[GC]GG
53 CCGGG
BfrI 3780 CTTAAG C^TTAAG
6790 CTTAAG
BsaI 1998 GAGACC GGTCTC(1/5)
5711 GAGACC
BsgI 1060 CTGCAC GTGCAG(16/14)
1148 GTGCAG
BspLU11I 810 ACATGT A^CATGT
6200 ACATGT
DsaI 4593 CCATGG C^C[GA][CT]GG
7264 CCATGG
EaeI 5799 TGGCCA [CT]^GGCC[GA]
7261 TGGCCA
EcoNI 3711 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
7508 CCTGTACCAGG
EcoRV 217 GATATC GAT^ATC
4107 GATATC
FbaI 5457 TGATCA T^GATCA
6832 TGATCA
HincII 3279 GTTGAC GT[CT]^[GA]AC
4264 GTTGAC
HindII 3279 GTTGAC GT[CT]^[GA]AC
4264 GTTGAC
MluNI 5799 TGGCCA TGG^CCA
7261 TGGCCA
MscI 5799 TGGCCA TGG^CCA
7261 TGGCCA
NciI 52 CCCGG CC^[GC]GG
53 CCGGG
NcoI 4593 CCATGG C^CATGG
7264 CCATGG
PflMI 5400 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5659 CCAGCAATTGG
PpuMI 899 AGGACCT [GA]G^G[AT]CC[CT]
3492 AGGTCCC
PstI 641 CTGCAG CTGCA^G
6183 CTGCAG
SpeI 945 ACTAGT A^CTAGT
5524 ACTAGT
Van91I 5400 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5659 CCAGCAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BciVI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI