##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AccI AclI AgeI Alw44I
AocI ApaLI AscI AvrII BamHI
BbrPI BglI BlnI BsaI BsiWI
BspHI BspLU11I Bst1107I BstBI BstZ17I
Bsu36I CpoI Eco81I FseI MluI
NaeI NgoMIV NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SalI SfiI
SfuI SgrAI SmaI SnaBI SphI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1918 GGTACC G^GTACC
ApaI 3403 GGGCCC GGGCC^C
Asp718I 1918 GGTACC G^GTACC
AviII 533 TGCGCA TGC^GCA
BcgI 4095 CGAAAACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4398 GTATCC GTATCC(6/5)
BclI 2670 TGATCA T^GATCA
BsaBI 1545 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2229 GTGCAG GTGCAG(16/14)
BsiEI 441 CGGCCG CG[GA][CT]^CG
BspDI 2104 ATCGAT AT^CGAT
BsrBI 709 GAGCGG CCGCTC(-3/-3)
BssHII 669 GCGCGC G^CGCGC
ClaI 2104 ATCGAT AT^CGAT
EagI 441 CGGCCG C^GGCCG
EclXI 441 CGGCCG C^GGCCG
Eco52I 441 CGGCCG C^GGCCG
EcoNI 698 CCTCGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3917 GAATTC G^AATTC
FbaI 2670 TGATCA T^GATCA
FspI 533 TGCGCA TGC^GCA
HindIII 7087 AAGCTT A^AGCTT
KpnI 1918 GGTACC GGTAC^C
MamI 1545 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 5080 GCTAGC G^CTAGC
NotI 440 GCGGCCGC GC^GGCCGC
NruI 919 TCGCGA TCG^CGA
PshAI 4546 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4359 GAGCTC GAGCT^C
SbfI 3871 CCTGCAGG CCTGCA^GG
SexAI 575 ACCAGGT A^CC[AT]GGT
Sse8387I 3871 CCTGCAGG CCTGCA^GG
StuI 5294 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 2266 TCCGGA T^CCGGA
3345 TCCGGA
AflII 6082 CTTAAG C^TTAAG
7803 CTTAAG
AhdI 1822 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1906 GACGGACGGTC
Aor51HI 3863 AGCGCT AGC^GCT
5083 AGCGCT
AspEI 1822 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1906 GACGGACGGTC
AspI 138 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3929 GACTTGGTC
BbeI 286 GGCGCC GGCGC^C
517 GGCGCC
BbvCI 4310 CCTCAGC CCTCAGC(-5/-2)
2374 GCTGAGG
BfrI 6082 CTTAAG C^TTAAG
7803 CTTAAG
BglII 4680 AGATCT A^GATCT
6062 AGATCT
BsaWI 2266 TCCGGA [AT]^CCGG[AT]
3345 TCCGGA
BseAI 2266 TCCGGA T^CCGGA
3345 TCCGGA
BsmBI 6971 CGTCTC CGTCTC(1/5)
1904 GAGACG
BspEI 2266 TCCGGA T^CCGGA
3345 TCCGGA
BsrFI 504 GCCGGT [GA]^CCGG[CT]
925 ACCGGC
BstEII 1439 GGTCACC G^GT[ACGT]ACC
6076 GGTAACC
BstPI 1439 GGTCACC G^GT[ACGT]ACC
6076 GGTAACC
Cfr10I 504 GCCGGT [GA]^CCGG[CT]
925 ACCGGC
DrdI 739 GACGTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4161 GACAACTGAGTC
Eam1105I 1822 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1906 GACGGACGGTC
Eco47III 3863 AGCGCT AGC^GCT
5083 AGCGCT
EcoO65I 1439 GGTCACC G^GT[ACGT]ACC
6076 GGTAACC
EcoRV 2122 GATATC GAT^ATC
3135 GATATC
HpaI 508 GTTAAC GTT^AAC
2625 GTTAAC
KasI 286 GGCGCC G^GCGCC
517 GGCGCC
MfeI 5106 CAATTG C^AATTG
6227 CAATTG
MroI 2266 TCCGGA T^CCGGA
3345 TCCGGA
MunI 5106 CAATTG C^AATTG
6227 CAATTG
NarI 286 GGCGCC GG^CGCC
517 GGCGCC
NcoI 1554 CCATGG C^CATGG
1719 CCATGG
PflFI 138 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3929 GACTTGGTC
PvuII 3474 CAGCTG CAG^CTG
3846 CAGCTG
SapI 564 GCTCTTC GCTCTTC(1/4)
5509 GCTCTTC
SfoI 286 GGCGCC GGC^GCC
517 GGCGCC
SpeI 1242 ACTAGT A^CTAGT
7896 ACTAGT
SwaI 1569 ATTTAAAT ATTT^AAAT
3329 ATTTAAAT
Tth111I 138 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3929 GACTTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PshBI PstI
RsaI SacII Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI