################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AccII AccIII AflIII AhdI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BglI BlnI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BssHII BstBI BstEII BstPI BstUI CfoI ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I FauI FbaI FseI FspI HaeII HhaI HinP1I KasI KpnI KspI MluI MroI MvnI NaeI NarI NgoMIV NotI NruI NspV PaeR7I PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI Sse8387I StuI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AclI 5654 AACGTT AA^CGTT AcyI 2531 GACGCC G[GA]^CG[CT]C AgeI 1870 ACCGGT A^CCGGT Alw44I 5478 GTGCAC G^TGCAC AocI 4752 CCTGAGG CC^T[ACGT]AGG ApaLI 5478 GTGCAC G^TGCAC AspI 5253 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 4439 CCCGGG C^[CT]CG[GA]G BanI 2582 GGTGCC G^G[CT][GA]CC BlpI 3472 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3472 GCTCAGC GC^T[ACGT]AGC BsaAI 2365 TACGTA [CT]AC^GT[GA] BsaHI 2531 GACGCC G[GA]^CG[CT]C BsaI 1536 GGTCTC GGTCTC(1/5) BseRI 7261 GAGGAG GAGGAG(10/8) BsoBI 4439 CCCGGG C^[CT]CG[GA]G BsrFI 1870 ACCGGT [GA]^CCGG[CT] BssSI 2431 CACGAG CACGAG(-5/-1) BstXI 706 CCACCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 4752 CCTGAGG CC^T[ACGT]AGG CelII 3472 GCTCAGC GC^T[ACGT]AGC Cfr10I 1870 ACCGGT [GA]^CCGG[CT] Eco81I 4752 CCTGAGG CC^T[ACGT]AGG Hin1I 2531 GACGCC G[GA]^CG[CT]C MspA1I 5180 CAGCTG C[AC]G^C[GT]G NheI 3477 GCTAGC G^CTAGC PflFI 5253 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1870 ACCGGT A^CCGGT Psp1406I 5654 AACGTT AA^CGTT PvuII 5180 CAGCTG CAG^CTG SmaI 4439 CCCGGG CCC^GGG SnaBI 2365 TACGTA TAC^GTA SphI 7602 GCATGC GCATG^C Tth111I 5253 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 4439 CCCGGG C^CCGGG XmaI 4439 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 3970 CCGC CCGC(-3/-1) 2948 GCGG BsaWI 1263 ACCGGA [AT]^CCGG[AT] 1870 ACCGGT BspMI 1332 ACCTGC ACCTGC(4/8) 2854 GCAGGT BsrDI 4513 GCAATG GCAATG(2/0) 4636 GCAATG BstAPI 7606 GCATAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 7714 GCACAAAATGC DraII 157 AGGACCT [GA]G^G[ACGT]CC[CT] 1617 GGGACCT DrdI 2531 GACGCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3718 GACTTCCGTGTC DsaI 2953 CCATGG C^C[GA][CT]GG 4790 CCATGG EcoO109I 157 AGGACCT [GA]G^G[ACGT]CC[CT] 1617 GGGACCT EcoRI 935 GAATTC G^AATTC 3200 GAATTC HgaI 2531 GACGC GACGC(5/10) 1318 GCGTC HindIII 2767 AAGCTT A^AGCTT 4835 AAGCTT HpaI 4259 GTTAAC GTT^AAC 5868 GTTAAC MfeI 204 CAATTG C^AATTG 1181 CAATTG MunI 204 CAATTG C^AATTG 1181 CAATTG NcoI 2953 CCATGG C^CATGG 4790 CCATGG PacI 287 TTAATTAA TTAAT^TAA 4596 TTAATTAA PpuMI 157 AGGACCT [GA]G^G[AT]CC[CT] 1617 GGGACCT SacI 2348 GAGCTC GAGCT^C 2417 GAGCTC SwaI 5628 ATTTAAAT ATTT^AAAT 8061 ATTTAAAT XcmI 468 CCAACCTGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3369 CCATATTCTGATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AcsI AfaI AflII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanII BbsI BbvI BciVI BcnI BfaI BfrI BglII BmyI BpmI BpuAI BsaBI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoRV EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshBI PstI RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SpeI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI