##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AflIII      
AhdI        Aor51HI     ApaI        AscI        Asp718I     
AspEI       AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BclI        BglI        
BlnI        BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BspLU11I    BsrBI       BssHII      
BstBI       BstEII      BstPI       BstUI       CfoI        
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
FauI        FbaI        FseI        FspI        HaeII       
HhaI        HinP1I      KasI        KpnI        KspI        
MluI        MroI        MvnI        NaeI        NarI        
NgoMIV      NotI        NruI        NspV        PaeR7I      
PmaCI       PmeI        PmlI        PshAI       PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       Sse8387I    
StuI        XhoI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AclI         5654  AACGTT               AA^CGTT
AcyI         2531  GACGCC               G[GA]^CG[CT]C
AgeI         1870  ACCGGT               A^CCGGT
Alw44I       5478  GTGCAC               G^TGCAC
AocI         4752  CCTGAGG              CC^T[ACGT]AGG
ApaLI        5478  GTGCAC               G^TGCAC
AspI         5253  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         4439  CCCGGG               C^[CT]CG[GA]G
BanI         2582  GGTGCC               G^G[CT][GA]CC
BlpI         3472  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3472  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2365  TACGTA               [CT]AC^GT[GA]
BsaHI        2531  GACGCC               G[GA]^CG[CT]C
BsaI         1536  GGTCTC               GGTCTC(1/5)
BseRI        7261  GAGGAG               GAGGAG(10/8)
BsoBI        4439  CCCGGG               C^[CT]CG[GA]G
BsrFI        1870  ACCGGT               [GA]^CCGG[CT]
BssSI        2431  CACGAG               CACGAG(-5/-1)
BstXI         706  CCACCCTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       4752  CCTGAGG              CC^T[ACGT]AGG
CelII        3472  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1870  ACCGGT               [GA]^CCGG[CT]
Eco81I       4752  CCTGAGG              CC^T[ACGT]AGG
Hin1I        2531  GACGCC               G[GA]^CG[CT]C
MspA1I       5180  CAGCTG               C[AC]G^C[GT]G
NheI         3477  GCTAGC               G^CTAGC
PflFI        5253  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1870  ACCGGT               A^CCGGT
Psp1406I     5654  AACGTT               AA^CGTT
PvuII        5180  CAGCTG               CAG^CTG
SmaI         4439  CCCGGG               CCC^GGG
SnaBI        2365  TACGTA               TAC^GTA
SphI         7602  GCATGC               GCATG^C
Tth111I      5253  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        4439  CCCGGG               C^CCGGG
XmaI         4439  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI         3970  CCGC                 CCGC(-3/-1)
             2948  GCGG                
BsaWI        1263  ACCGGA               [AT]^CCGG[AT]
             1870  ACCGGT              
BspMI        1332  ACCTGC               ACCTGC(4/8)
             2854  GCAGGT              
BsrDI        4513  GCAATG               GCAATG(2/0)
             4636  GCAATG              
BstAPI       7606  GCATAAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             7714  GCACAAAATGC         
DraII         157  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1617  GGGACCT             
DrdI         2531  GACGCCACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3718  GACTTCCGTGTC        
DsaI         2953  CCATGG               C^C[GA][CT]GG
             4790  CCATGG              
EcoO109I      157  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1617  GGGACCT             
EcoRI         935  GAATTC               G^AATTC
             3200  GAATTC              
HgaI         2531  GACGC                GACGC(5/10)
             1318  GCGTC               
HindIII      2767  AAGCTT               A^AGCTT
             4835  AAGCTT              
HpaI         4259  GTTAAC               GTT^AAC
             5868  GTTAAC              
MfeI          204  CAATTG               C^AATTG
             1181  CAATTG              
MunI          204  CAATTG               C^AATTG
             1181  CAATTG              
NcoI         2953  CCATGG               C^CATGG
             4790  CCATGG              
PacI          287  TTAATTAA             TTAAT^TAA
             4596  TTAATTAA            
PpuMI         157  AGGACCT              [GA]G^G[AT]CC[CT]
             1617  GGGACCT             
SacI         2348  GAGCTC               GAGCT^C
             2417  GAGCTC              
SwaI         5628  ATTTAAAT             ATTT^AAAT
             8061  ATTTAAAT            
XcmI          468  CCAACCTGGATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3369  CCATATTCTGATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccI        AcsI        AfaI        AflII       AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AspHI       AvaII       BalI        BanII       
BbsI        BbvI        BciVI       BcnI        BfaI        
BfrI        BglII       BmyI        BpmI        BpuAI       
BsaBI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
Bsp1407I    BspHI       BsrGI       BsrI        BssKI       
Bst1107I    BstF5I      BstNI       BstYI       BstZ17I     
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EaeI        EarI        EcoRII      EcoRV       EcoT14I     
EcoT22I     Fnu4HI      FokI        HaeIII      HapII       
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NdeI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PshBI       PstI        RcaI        RsaI        
SapI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        SpeI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XhoII       XmnI