##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccII AccIII AflIII
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BclI BglI
BlnI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BssHII
BstBI BstEII BstPI BstUI CfoI
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
FauI FbaI FseI FspI HaeII
HhaI HinP1I KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NgoMIV NotI NruI NspV PaeR7I
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI Sse8387I
StuI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 5654 AACGTT AA^CGTT
AcyI 2531 GACGCC G[GA]^CG[CT]C
AgeI 1870 ACCGGT A^CCGGT
Alw44I 5478 GTGCAC G^TGCAC
AocI 4752 CCTGAGG CC^T[ACGT]AGG
ApaLI 5478 GTGCAC G^TGCAC
AspI 5253 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4439 CCCGGG C^[CT]CG[GA]G
BanI 2582 GGTGCC G^G[CT][GA]CC
BlpI 3472 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3472 GCTCAGC GC^T[ACGT]AGC
BsaAI 2365 TACGTA [CT]AC^GT[GA]
BsaHI 2531 GACGCC G[GA]^CG[CT]C
BsaI 1536 GGTCTC GGTCTC(1/5)
BseRI 7261 GAGGAG GAGGAG(10/8)
BsoBI 4439 CCCGGG C^[CT]CG[GA]G
BsrFI 1870 ACCGGT [GA]^CCGG[CT]
BssSI 2431 CACGAG CACGAG(-5/-1)
BstXI 706 CCACCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4752 CCTGAGG CC^T[ACGT]AGG
CelII 3472 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1870 ACCGGT [GA]^CCGG[CT]
Eco81I 4752 CCTGAGG CC^T[ACGT]AGG
Hin1I 2531 GACGCC G[GA]^CG[CT]C
MspA1I 5180 CAGCTG C[AC]G^C[GT]G
NheI 3477 GCTAGC G^CTAGC
PflFI 5253 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1870 ACCGGT A^CCGGT
Psp1406I 5654 AACGTT AA^CGTT
PvuII 5180 CAGCTG CAG^CTG
SmaI 4439 CCCGGG CCC^GGG
SnaBI 2365 TACGTA TAC^GTA
SphI 7602 GCATGC GCATG^C
Tth111I 5253 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4439 CCCGGG C^CCGGG
XmaI 4439 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 3970 CCGC CCGC(-3/-1)
2948 GCGG
BsaWI 1263 ACCGGA [AT]^CCGG[AT]
1870 ACCGGT
BspMI 1332 ACCTGC ACCTGC(4/8)
2854 GCAGGT
BsrDI 4513 GCAATG GCAATG(2/0)
4636 GCAATG
BstAPI 7606 GCATAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
7714 GCACAAAATGC
DraII 157 AGGACCT [GA]G^G[ACGT]CC[CT]
1617 GGGACCT
DrdI 2531 GACGCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3718 GACTTCCGTGTC
DsaI 2953 CCATGG C^C[GA][CT]GG
4790 CCATGG
EcoO109I 157 AGGACCT [GA]G^G[ACGT]CC[CT]
1617 GGGACCT
EcoRI 935 GAATTC G^AATTC
3200 GAATTC
HgaI 2531 GACGC GACGC(5/10)
1318 GCGTC
HindIII 2767 AAGCTT A^AGCTT
4835 AAGCTT
HpaI 4259 GTTAAC GTT^AAC
5868 GTTAAC
MfeI 204 CAATTG C^AATTG
1181 CAATTG
MunI 204 CAATTG C^AATTG
1181 CAATTG
NcoI 2953 CCATGG C^CATGG
4790 CCATGG
PacI 287 TTAATTAA TTAAT^TAA
4596 TTAATTAA
PpuMI 157 AGGACCT [GA]G^G[AT]CC[CT]
1617 GGGACCT
SacI 2348 GAGCTC GAGCT^C
2417 GAGCTC
SwaI 5628 ATTTAAAT ATTT^AAAT
8061 ATTTAAAT
XcmI 468 CCAACCTGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3369 CCATATTCTGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanII
BbsI BbvI BciVI BcnI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaBI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstYI BstZ17I
Cac8I Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoRV EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PshBI PstI RcaI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SpeI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmnI