################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AatII AccIII AcyI AflIII AgeI AhdI AscI AspEI AviII BbeI BbrPI BsaAI BsaHI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BssHII BssSI BstBI ClaI CpoI DrdI Eam1105I EcoRV FseI FspI Hin1I HpaI KasI MluI MroI NarI NotI NruI NspV PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SalI SbfI SfoI SfuI SgrAI SnaBI Sse8387I ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 7564 GGTACC G^GTACC AclI 732 AACGTT AA^CGTT Alw44I 4035 GTGCAC G^TGCAC Aor51HI 429 AGCGCT AGC^GCT ApaLI 4035 GTGCAC G^TGCAC Asp718I 7564 GGTACC G^GTACC BbvCI 3618 CCTCAGC CCTCAGC(-5/-2) BcgI 7161 GCAATGAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1320 AGATCT A^GATCT BsiEI 197 CGGCCG CG[GA][CT]^CG Bsp1407I 7463 TGTACA T^GTACA BspHI 4077 TCATGA T^CATGA BsrGI 7463 TGTACA T^GTACA Bst1107I 1489 GTATAC GTA^TAC BstAPI 5634 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2115 CCAGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1489 GTATAC GTA^TAC DraIII 3260 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 197 CGGCCG C^GGCCG EclXI 197 CGGCCG C^GGCCG Eco47III 429 AGCGCT AGC^GCT Eco52I 197 CGGCCG C^GGCCG EcoNI 773 CCTTCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 6677 GAATTC G^AATTC HaeII 429 AGCGCT [GA]GCGC^[CT] KpnI 7564 GGTACC GGTAC^C MfeI 1787 CAATTG C^AATTG MunI 1787 CAATTG C^AATTG NdeI 4040 CATATG CA^TATG NheI 6555 GCTAGC G^CTAGC PaeR7I 777 CTCGAG C^TCGAG Psp1406I 732 AACGTT AA^CGTT RcaI 4077 TCATGA T^CATGA SacI 5652 GAGCTC GAGCT^C SexAI 6667 ACCTGGT A^CC[AT]GGT SfiI 80 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 313 CCCGGG CCC^GGG SpeI 7094 ACTAGT A^CTAGT SspBI 7463 TGTACA T^GTACA StuI 2177 AGGCCT AGG^CCT SwaI 7395 ATTTAAAT ATTT^AAAT XbaI 3626 TCTAGA T^CTAGA XhoI 777 CTCGAG C^TCGAG XmaCI 313 CCCGGG C^CCGGG XmaI 313 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 1056 GTCTAC GT^[AC][GT]AC 1489 GTATAC Asp700I 4516 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5164 GAAAATGTTC AspI 1970 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5952 GACATGGTC AvrII 2907 CCTAGG C^CTAGG 4593 CCTAGG BalI 2420 TGGCCA TGG^CCA 3092 TGGCCA BamHI 1299 GGATCC G^GATCC 7571 GGATCC BlnI 2907 CCTAGG C^CTAGG 4593 CCTAGG BlpI 4630 GCTGAGC GC^T[ACGT]AGC 7330 GCTGAGC Bpu1102I 4630 GCTGAGC GC^T[ACGT]AGC 7330 GCTGAGC BsaBI 1511 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2096 GATCAGTATC BsaI 2756 GAGACC GGTCTC(1/5) 5783 GAGACC BsrBI 63 CCGCTC CCGCTC(-3/-3) 1331 GAGCGG BsrFI 81 GCCGGC [GA]^CCGG[CT] 2076 GCCGGC BstEII 4205 GGTGACC G^GT[ACGT]ACC 6853 GGTCACC BstPI 4205 GGTGACC G^GT[ACGT]ACC 6853 GGTCACC CelII 4630 GCTGAGC GC^T[ACGT]AGC 7330 GCTGAGC Cfr10I 81 GCCGGC [GA]^CCGG[CT] 2076 GCCGGC EcoO65I 4205 GGTGACC G^GT[ACGT]ACC 6853 GGTCACC HindIII 577 AAGCTT A^AGCTT 1997 AAGCTT MamI 1511 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2096 GATCAGTATC MluNI 2420 TGGCCA TGG^CCA 3092 TGGCCA MscI 2420 TGGCCA TGG^CCA 3092 TGGCCA NaeI 81 GCCGGC GCC^GGC 2076 GCCGGC NcoI 2849 CCATGG C^CATGG 3222 CCATGG NgoMIV 81 GCCGGC G^CCGGC 2076 GCCGGC PacI 6515 TTAATTAA TTAAT^TAA 6534 TTAATTAA PflFI 1970 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5952 GACATGGTC ScaI 32 AGTACT AGT^ACT 712 AGTACT SphI 3365 GCATGC GCATG^C 7657 GCATGC Tth111I 1970 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5952 GACATGGTC XmnI 4516 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5164 GAAAATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApaI ApoI AseI AsnI AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BfrI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PshBI PstI PvuII RsaI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII