################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII Acc65I AclI AgeI Aor51HI AscI Asp718I BbeI BbrPI BcgI BglI BsaAI BsiEI BsiWI BspDI BssHII BstBI ClaI CpoI DrdI EagI EclXI Eco47III Eco52I FseI HaeII KasI KpnI KspI MluI NarI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 8118 TCCGGA T^CCGGA AflII 5379 CTTAAG C^TTAAG AspI 4512 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 6543 GGATAC GTATCC(6/5) BfrI 5379 CTTAAG C^TTAAG BlpI 5702 GCTGAGC GC^T[ACGT]AGC Bpu1102I 5702 GCTGAGC GC^T[ACGT]AGC BsaWI 8118 TCCGGA [AT]^CCGG[AT] BseAI 8118 TCCGGA T^CCGGA BspEI 8118 TCCGGA T^CCGGA BsrFI 822 GCCGGC [GA]^CCGG[CT] BssSI 1917 CACGAG CACGAG(-5/-1) Bst1107I 3788 GTATAC GTA^TAC BstZ17I 3788 GTATAC GTA^TAC CelII 5702 GCTGAGC GC^T[ACGT]AGC Cfr10I 822 GCCGGC [GA]^CCGG[CT] EcoRI 3527 GAATTC G^AATTC EcoT22I 120 ATGCAT ATGCA^T FauI 2064 CCCGC CCCGC(4/6) HindIII 7499 AAGCTT A^AGCTT HpaI 3271 GTTAAC GTT^AAC MfeI 3298 CAATTG C^AATTG MroI 8118 TCCGGA T^CCGGA MunI 3298 CAATTG C^AATTG NaeI 822 GCCGGC GCC^GGC NdeI 1095 CATATG CA^TATG NgoMIV 822 GCCGGC G^CCGGC NsiI 120 ATGCAT ATGCA^T PflFI 4512 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1223 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 3328 GAGCTC GAGCT^C SapI 1738 GAAGAGC GCTCTTC(1/4) SmaI 1959 CCCGGG CCC^GGG Tth111I 4512 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1959 CCCGGG C^CCGGG XmaI 1959 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 2066 CGCG CG^CG 4410 CGCG AcyI 1325 GACGCC G[GA]^CG[CT]C 2027 GACGCC AhdI 686 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1019 GACAGCTGGTC ApaI 830 GGGCCC GGGCC^C 2059 GGGCCC AseI 4941 ATTAAT AT^TAAT 5982 ATTAAT AsnI 4941 ATTAAT AT^TAAT 5982 ATTAAT Asp700I 5149 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5509 GAAGCAATTC AspEI 686 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1019 GACAGCTGGTC AviII 720 TGCGCA TGC^GCA 6819 TGCGCA BclI 531 TGATCA T^GATCA 2163 TGATCA BsaBI 4736 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 8026 GATTTGAATC BsaHI 1325 GACGCC G[GA]^CG[CT]C 2027 GACGCC BsaI 730 GAGACC GGTCTC(1/5) 6134 GAGACC BsmBI 6328 CGTCTC CGTCTC(1/5) 6374 CGTCTC BstEII 2823 GGTTACC G^GT[ACGT]ACC 5494 GGTAACC BstPI 2823 GGTTACC G^GT[ACGT]ACC 5494 GGTAACC BstUI 2066 CGCG CG^CG 4410 CGCG DraIII 1427 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG 3564 CACCATGTG Eam1105I 686 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1019 GACAGCTGGTC EcoO65I 2823 GGTTACC G^GT[ACGT]ACC 5494 GGTAACC EcoRV 620 GATATC GAT^ATC 2657 GATATC FbaI 531 TGATCA T^GATCA 2163 TGATCA FspI 720 TGCGCA TGC^GCA 6819 TGCGCA Hin1I 1325 GACGCC G[GA]^CG[CT]C 2027 GACGCC MamI 4736 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 8026 GATTTGAATC MvnI 2066 CGCG CG^CG 4410 CGCG PshBI 4941 ATTAAT AT^TAAT 5982 ATTAAT ScaI 4848 AGTACT AGT^ACT 5045 AGTACT SphI 3916 GCATGC GCATG^C 4361 GCATGC StuI 713 AGGCCT AGG^CCT 3936 AGGCCT XmnI 5149 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5509 GAAGCAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BlnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII