##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI Asp718I BbeI BbrPI BcgI
BglI BsaAI BsiEI BsiWI BspDI
BssHII BstBI ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I FseI
HaeII KasI KpnI KspI MluI
NarI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 8118 TCCGGA T^CCGGA
AflII 5379 CTTAAG C^TTAAG
AspI 4512 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 6543 GGATAC GTATCC(6/5)
BfrI 5379 CTTAAG C^TTAAG
BlpI 5702 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5702 GCTGAGC GC^T[ACGT]AGC
BsaWI 8118 TCCGGA [AT]^CCGG[AT]
BseAI 8118 TCCGGA T^CCGGA
BspEI 8118 TCCGGA T^CCGGA
BsrFI 822 GCCGGC [GA]^CCGG[CT]
BssSI 1917 CACGAG CACGAG(-5/-1)
Bst1107I 3788 GTATAC GTA^TAC
BstZ17I 3788 GTATAC GTA^TAC
CelII 5702 GCTGAGC GC^T[ACGT]AGC
Cfr10I 822 GCCGGC [GA]^CCGG[CT]
EcoRI 3527 GAATTC G^AATTC
EcoT22I 120 ATGCAT ATGCA^T
FauI 2064 CCCGC CCCGC(4/6)
HindIII 7499 AAGCTT A^AGCTT
HpaI 3271 GTTAAC GTT^AAC
MfeI 3298 CAATTG C^AATTG
MroI 8118 TCCGGA T^CCGGA
MunI 3298 CAATTG C^AATTG
NaeI 822 GCCGGC GCC^GGC
NdeI 1095 CATATG CA^TATG
NgoMIV 822 GCCGGC G^CCGGC
NsiI 120 ATGCAT ATGCA^T
PflFI 4512 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1223 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3328 GAGCTC GAGCT^C
SapI 1738 GAAGAGC GCTCTTC(1/4)
SmaI 1959 CCCGGG CCC^GGG
Tth111I 4512 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1959 CCCGGG C^CCGGG
XmaI 1959 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 2066 CGCG CG^CG
4410 CGCG
AcyI 1325 GACGCC G[GA]^CG[CT]C
2027 GACGCC
AhdI 686 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1019 GACAGCTGGTC
ApaI 830 GGGCCC GGGCC^C
2059 GGGCCC
AseI 4941 ATTAAT AT^TAAT
5982 ATTAAT
AsnI 4941 ATTAAT AT^TAAT
5982 ATTAAT
Asp700I 5149 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5509 GAAGCAATTC
AspEI 686 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1019 GACAGCTGGTC
AviII 720 TGCGCA TGC^GCA
6819 TGCGCA
BclI 531 TGATCA T^GATCA
2163 TGATCA
BsaBI 4736 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8026 GATTTGAATC
BsaHI 1325 GACGCC G[GA]^CG[CT]C
2027 GACGCC
BsaI 730 GAGACC GGTCTC(1/5)
6134 GAGACC
BsmBI 6328 CGTCTC CGTCTC(1/5)
6374 CGTCTC
BstEII 2823 GGTTACC G^GT[ACGT]ACC
5494 GGTAACC
BstPI 2823 GGTTACC G^GT[ACGT]ACC
5494 GGTAACC
BstUI 2066 CGCG CG^CG
4410 CGCG
DraIII 1427 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3564 CACCATGTG
Eam1105I 686 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1019 GACAGCTGGTC
EcoO65I 2823 GGTTACC G^GT[ACGT]ACC
5494 GGTAACC
EcoRV 620 GATATC GAT^ATC
2657 GATATC
FbaI 531 TGATCA T^GATCA
2163 TGATCA
FspI 720 TGCGCA TGC^GCA
6819 TGCGCA
Hin1I 1325 GACGCC G[GA]^CG[CT]C
2027 GACGCC
MamI 4736 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8026 GATTTGAATC
MvnI 2066 CGCG CG^CG
4410 CGCG
PshBI 4941 ATTAAT AT^TAAT
5982 ATTAAT
ScaI 4848 AGTACT AGT^ACT
5045 AGTACT
SphI 3916 GCATGC GCATG^C
4361 GCATGC
StuI 713 AGGCCT AGG^CCT
3936 AGGCCT
XmnI 5149 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5509 GAAGCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BlnI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII