##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I Aor51HI ApaI AscI AseI
AsnI Asp718I AviII BbeI BsiWI
Bsp1407I BspDI BsrBI BsrGI BstBI
ClaI EagI EclXI Eco47III Eco52I
FseI FspI KasI KpnI MfeI
MluI MunI NarI NheI NotI
NspV PacI PflMI PmeI PshBI
PvuI SalI SbfI SfiI SfoI
SfuI SnaBI SpeI Sse8387I SspBI
SwaI Van91I
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 2803 GACGTC GACGT^C
AflII 2761 CTTAAG C^TTAAG
AocI 4054 CCTGAGG CC^T[ACGT]AGG
Asp700I 6971 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4534 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6798 CCTAGG C^CTAGG
BbvCI 599 GCTGAGG CCTCAGC(-5/-2)
BclI 6987 TGATCA T^GATCA
BfrI 2761 CTTAAG C^TTAAG
BlnI 6798 CCTAGG C^CTAGG
BsiEI 2346 CGACCG CG[GA][CT]^CG
BsmBI 837 GAGACG CGTCTC(1/5)
BspLU11I 2531 ACATGT A^CATGT
BssHII 584 GCGCGC G^CGCGC
Bst1107I 3022 GTATAC GTA^TAC
BstEII 4728 GGTGACC G^GT[ACGT]ACC
BstPI 4728 GGTGACC G^GT[ACGT]ACC
BstZ17I 3022 GTATAC GTA^TAC
Bsu36I 4054 CCTGAGG CC^T[ACGT]AGG
DraIII 2632 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2734 GACCAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4054 CCTGAGG CC^T[ACGT]AGG
EcoNI 7719 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4728 GGTGACC G^GT[ACGT]ACC
FbaI 6987 TGATCA T^GATCA
HpaI 4210 GTTAAC GTT^AAC
NcoI 1064 CCATGG C^CATGG
PflFI 4534 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 6299 GAGCTC GAGCT^C
ScaI 2405 AGTACT AGT^ACT
SgrAI 6426 CACCGGTG C[GA]^CCGG[CT]G
SspI 3798 AATATT AAT^ATT
Tth111I 4534 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 6971 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccI 3022 GTATAC GT^[AC][GT]AC
7638 GTCTAC
AccIII 885 TCCGGA T^CCGGA
3062 TCCGGA
AclI 2188 AACGTT AA^CGTT
2210 AACGTT
AhdI 839 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6046 GACTGCTTGTC
Alw44I 1468 GTGCAC G^TGCAC
3406 GTGCAC
AlwNI 385 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1203 CAGGTTCTG
ApaLI 1468 GTGCAC G^TGCAC
3406 GTGCAC
AspEI 839 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6046 GACTGCTTGTC
BanI 522 GGTGCC G^G[CT][GA]CC
4873 GGTGCC
BcgI 2067 CGAGTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
338 GCACGGGCCTCG
BseAI 885 TCCGGA T^CCGGA
3062 TCCGGA
BspEI 885 TCCGGA T^CCGGA
3062 TCCGGA
BspHI 2009 TCATGA T^CATGA
2717 TCATGA
BsrDI 764 CATTGC GCAATG(2/0)
7094 CATTGC
CpoI 436 CGGTCCG CG^G[AT]CCG
845 CGGTCCG
DraI 3747 TTTAAA TTT^AAA
5774 TTTAAA
Eam1105I 839 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6046 GACTGCTTGTC
EcoRI 4684 GAATTC G^AATTC
5327 GAATTC
EcoRV 4933 GATATC GAT^ATC
6437 GATATC
EcoT22I 1489 ATGCAT ATGCA^T
7463 ATGCAT
HaeII 3383 AGCGCC [GA]GCGC^[CT]
3551 AGCGCC
HincII 1669 GTCAAC GT[CT]^[GA]AC
4210 GTTAAC
HindII 1669 GTCAAC GT[CT]^[GA]AC
4210 GTTAAC
HindIII 5819 AAGCTT A^AGCTT
6958 AAGCTT
KspI 512 CCGCGG CCGC^GG
730 CCGCGG
MroI 885 TCCGGA T^CCGGA
3062 TCCGGA
NaeI 612 GCCGGC GCC^GGC
5419 GCCGGC
NdeI 5317 CATATG CA^TATG
7607 CATATG
NgoMIV 612 GCCGGC G^CCGGC
5419 GCCGGC
NruI 528 TCGCGA TCG^CGA
3210 TCGCGA
NsiI 1489 ATGCAT ATGCA^T
7463 ATGCAT
PaeR7I 346 CTCGAG C^TCGAG
2843 CTCGAG
Psp1406I 2188 AACGTT AA^CGTT
2210 AACGTT
RcaI 2009 TCATGA T^CATGA
2717 TCATGA
RsrII 436 CGGTCCG CG^G[AT]CCG
845 CGGTCCG
SacII 512 CCGCGG CCGC^GG
730 CCGCGG
SexAI 1201 ACCAGGT A^CC[AT]GGT
1475 ACCTGGT
SmaI 284 CCCGGG CCC^GGG
628 CCCGGG
SphI 2648 GCATGC GCATG^C
7461 GCATGC
StuI 5456 AGGCCT AGG^CCT
5556 AGGCCT
XbaI 12 TCTAGA T^CTAGA
5215 TCTAGA
XcmI 3260 CCATGTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7492 CCACCGTCTTGGTGG
XhoI 346 CTCGAG C^TCGAG
2843 CTCGAG
XmaCI 284 CCCGGG C^CCGGG
628 CCCGGG
XmaI 284 CCCGGG C^CCGGG
628 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AgeI AluI AlwI ApoI
AspHI AvaI AvaII BalI BamHI
BanII BbrPI BbsI BbvI BciVI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PinAI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII