##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       ApoI        
AseI        AsnI        Asp718I     BamHI       BciVI       
BclI        BfrI        BsaBI       BsiWI       BspDI       
BsrDI       Bst1107I    BstZ17I     ClaI        CpoI        
EcoRI       EcoRV       FbaI        HpaI        KpnI        
MamI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        PacI        PmeI        
PshBI       Psp1406I    PvuI        RsrII       SalI        
SbfI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        2044  GACGTC               GACGT^C
AccI         4232  GTCTAC               GT^[AC][GT]AC
AgeI         1974  ACCGGT               A^CCGGT
AhdI          269  GACTGAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4307  GTGCAC               G^TGCAC
ApaLI        4307  GTGCAC               G^TGCAC
AscI         1059  GGCGCGCC             GG^CGCGCC
AspEI         269  GACTGAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3731  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        5403  CCTAGG               C^CTAGG
BglII        1357  AGATCT               A^GATCT
BlnI         5403  CCTAGG               C^CTAGG
BlpI         4939  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     4939  GCTGAGC              GC^T[ACGT]AGC
BsmBI        5653  CGTCTC               CGTCTC(1/5)
Bsp1407I     4032  TGTACA               T^GTACA
BsrGI        4032  TGTACA               T^GTACA
BstAPI       3361  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        3621  TTCGAA               TT^CGAA
BstEII       3829  GGTCACC              G^GT[ACGT]ACC
BstPI        3829  GGTCACC              G^GT[ACGT]ACC
CelII        4939  GCTGAGC              GC^T[ACGT]AGC
DraI         5397  TTTAAA               TTT^AAA
DrdI         1318  GACTCAGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      269  GACTGAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3829  GGTCACC              G^GT[ACGT]ACC
EcoT22I      5940  ATGCAT               ATGCA^T
HindIII      3661  AAGCTT               A^AGCTT
NsiI         5940  ATGCAT               ATGCA^T
NspV         3621  TTCGAA               TT^CGAA
PflFI        3731  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1974  ACCGGT               A^CCGGT
SfiI          364  GGCCGTGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         3621  TTCGAA               TT^CGAA
SspBI        4032  TGTACA               T^GTACA
TfiI         1412  GATTC                G^A[AT]TC
Tsp509I      5479  AATT                 ^AATT
Tth111I      3731  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII       1396  TCCGGA               T^CCGGA
             3972  TCCGGA              
AflIII       4065  ACATGT               A^C[GA][CT]GT
             6011  ACATGT              
AocI          165  CCTGAGG              CC^T[ACGT]AGG
             5812  CCTCAGG             
Asp700I      1930  GAAGTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4270  GAAGGTGTTC          
BbrPI        2526  CACGTG               CAC^GTG
             5922  CACGTG              
BseAI        1396  TCCGGA               T^CCGGA
             3972  TCCGGA              
BsmI         3913  GAATGC               GAATGC(1/-1)
             5942  GCATTC              
BspEI        1396  TCCGGA               T^CCGGA
             3972  TCCGGA              
BspLU11I     4065  ACATGT               A^CATGT
             6011  ACATGT              
Bsu36I        165  CCTGAGG              CC^T[ACGT]AGG
             5812  CCTCAGG             
Eco81I        165  CCTGAGG              CC^T[ACGT]AGG
             5812  CCTCAGG             
FseI          561  GGCCGGCC             GGCCGG^CC
             3323  GGCCGGCC            
HincII       4484  GTCAAC               GT[CT]^[GA]AC
             5448  GTCAAC              
HindII       4484  GTCAAC               GT[CT]^[GA]AC
             5448  GTCAAC              
KspI          515  CCGCGG               CCGC^GG
              567  CCGCGG              
MroI         1396  TCCGGA               T^CCGGA
             3972  TCCGGA              
MseI          721  TTAA                 T^TAA
             5398  TTAA                
NspI         4065  ACATGT               [GA]CATG^[CT]
             6011  ACATGT              
PmaCI        2526  CACGTG               CAC^GTG
             5922  CACGTG              
PmlI         2526  CACGTG               CAC^GTG
             5922  CACGTG              
PshAI         449  GACCCCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              695  GACTCCAGTC          
SacII         515  CCGCGG               CCGC^GG
              567  CCGCGG              
ScaI         3882  AGTACT               AGT^ACT
             4566  AGTACT              
SexAI        2377  ACCAGGT              A^CC[AT]GGT
             3732  ACCTGGT             
Tru9I         721  TTAA                 T^TAA
             5398  TTAA                
XbaI         1797  TCTAGA               T^CTAGA
             5515  TCTAGA              
XmnI         1930  GAAGTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4270  GAAGGTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  145 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApaI        AspHI       
AvaI        AvaII       AviII       BalI        BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcgI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspHI       BspMI       BsrBI       
BsrFI       BsrI        BssHII      BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EagI        EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       PaeR7I      
PflMI       PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        StuI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XhoI        XhoII       XmaCI       XmaI