##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI Asp718I AspEI
BalI BciVI BfrI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI HpaI KpnI MluI
MluNI MroI MscI NdeI NheI
NotI NruI NsiI PacI PinAI
PmeI Psp1406I PvuI RsrII SbfI
SfiI SgrAI SmaI SnaBI Sse8387I
SspI SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 4919 GACGTC GACGT^C
ApaI 5522 GGGCCC GGGCC^C
AviII 981 TGCGCA TGC^GCA
AvrII 4493 CCTAGG C^CTAGG
BamHI 3874 GGATCC G^GATCC
BbeI 3441 GGCGCC GGCGC^C
BbrPI 1811 CACGTG CAC^GTG
BcgI 3052 GCAGGGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5224 TGATCA T^GATCA
BlnI 4493 CCTAGG C^CTAGG
BlpI 2641 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2641 GCTCAGC GC^T[ACGT]AGC
BsaBI 1607 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 1061 ACATGT A^CATGT
BsrFI 4289 GCCGGC [GA]^CCGG[CT]
BssSI 4588 CACGAG CACGAG(-5/-1)
BstBI 1638 TTCGAA TT^CGAA
CelII 2641 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4289 GCCGGC [GA]^CCGG[CT]
DrdI 511 GACAAGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 5224 TGATCA T^GATCA
FspI 981 TGCGCA TGC^GCA
HindIII 745 AAGCTT A^AGCTT
KasI 3441 GGCGCC G^GCGCC
KspI 3182 CCGCGG CCGC^GG
MamI 1607 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4289 GCCGGC GCC^GGC
NarI 3441 GGCGCC GG^CGCC
NgoMIV 4289 GCCGGC G^CCGGC
NspV 1638 TTCGAA TT^CGAA
PmaCI 1811 CACGTG CAC^GTG
PmlI 1811 CACGTG CAC^GTG
PshAI 1206 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3182 CCGCGG CCGC^GG
SalI 1756 GTCGAC G^TCGAC
ScaI 1468 AGTACT AGT^ACT
SfoI 3441 GGCGCC GGC^GCC
SfuI 1638 TTCGAA TT^CGAA
SpeI 1706 ACTAGT A^CTAGT
SphI 3210 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 3183 CGCG CG^CG
3357 CGCG
AflIII 1061 ACATGT A^C[GA][CT]GT
1534 ACACGT
AseI 2450 ATTAAT AT^TAAT
3862 ATTAAT
AsnI 2450 ATTAAT AT^TAAT
3862 ATTAAT
AspI 1043 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2324 GACAGTGTC
BsaWI 201 TCCGGT [AT]^CCGG[AT]
4239 ACCGGA
Bsp1407I 1968 TGTACA T^GTACA
2103 TGTACA
BspMI 5394 ACCTGC ACCTGC(4/8)
5663 ACCTGC
BsrGI 1968 TGTACA T^GTACA
2103 TGTACA
BstUI 3183 CGCG CG^CG
3357 CGCG
BstXI 2230 CCAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4380 CCAGCCCAGTGG
DraI 26 TTTAAA TTT^AAA
5708 TTTAAA
EcoNI 4133 CCTCGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5395 CCTGCGAGAGG
EcoRV 614 GATATC GAT^ATC
2210 GATATC
HincII 1756 GTCGAC GT[CT]^[GA]AC
2987 GTCAAC
HindII 1756 GTCGAC GT[CT]^[GA]AC
2987 GTCAAC
MvnI 3183 CGCG CG^CG
3357 CGCG
PaeR7I 3773 CTCGAG C^TCGAG
3928 CTCGAG
PflFI 1043 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2324 GACAGTGTC
PflMI 2658 CCATTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4228 CCACGGCCTGG
PshBI 2450 ATTAAT AT^TAAT
3862 ATTAAT
SapI 4421 GAAGAGC GCTCTTC(1/4)
581 GCTCTTC
SexAI 2351 ACCAGGT A^CC[AT]GGT
5127 ACCTGGT
SspBI 1968 TGTACA T^GTACA
2103 TGTACA
Tth111I 1043 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2324 GACAGTGTC
Van91I 2658 CCATTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4228 CCACGGCCTGG
XhoI 3773 CTCGAG C^TCGAG
3928 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI